Inhibitory effect of naringenin via IL‐13 level regulation on thymic stromal lymphopoietin‐induced inflammatory reactions

Abstract

Naringenin (NG) has various beneficial properties, such as anti-cancer and anti-inflammatory effects. Thymic stromal lymphopoietin (TSLP) induces mast cell proliferation and inflammatory reactions. The aim of this study was to investigate the regulatory effect of NG on TSLP-induced mast cell proliferation and inflammatory reactions using human mast cell line (HMC-1) cells. HMC-1 cells were pre-treated with NG and then treated with TSLP. HMC-1 cells proliferation was determined by quantifying bromodeoxyuridine incorporation. Levels of anti-apoptotic and pro-apoptotic factors were analyzed by western blot analysis. The productions and mRNA expressions of interleukin (IL)-13 and tumour necrosis factor-α (TNF-α) were analyzed by ELISA and quantitative real-time PCR. We found that NG significantly attenuated HMC-1 cells proliferation and Ki-67 mRNA expression promoted by TSLP. NG significantly suppressed mRNA expression of TSLP receptor and IL-7 receptor α in TSLP-treated HMC-1 cells. NG significantly down-regulated levels of phosphorylated-signal transducer and activation of transcription 6 and murine double-minute 2 in TSLP-treated HMC-1 cells, up-regulated levels of cleaved poly ADP-ribose polymerase and p53 in TSLP-treated HMC-1 cells. Furthermore, NG significantly decreased the productions and mRNA expressions of IL-13 and TNF-α in TSLP-treated HMC-1 cells. These results suggest NG has an inhibitory effect on mast cell-mediated allergic inflammatory reactions.

Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells

“…Cytokine assay. HMc-1 cells (3x10 5 ) were pretreated with UA (0.002-0.2 µg/ml) for 1 h prior to stimulation with 0.05 µM PMA plus 1 µM calcium ionophore (PMAcI), and then incubated for 7 h. TSLP and TNF-α levels were assessed in the culture supernatants using ELISA, as described previously (24).…”

Effects of Dietary Components on Mast Cells: Possible Use as Nutraceuticals for Allergies?

“…MC-mediated inflammatory responses were suppressed by naringenin by downregulating TSLP-induced increase in IL-13 and TNF-α levels by almost 40% in MC. Therefore, naringenin may have an antiproliferative effect by regulating the levels of anti-apoptotic and proapoptotic factors in MC [60]. In an OVA-induced allergic rhinitis model, oral treatment with naringenin improved nasal symptoms and decreased serum total IgE and Th2 cytokines IL-4 and IL-5 compared with the OVA treated group [86].…”

Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells

“…Real-time PCR was performed by using a Power SYBR Green PCR master mix. ABI StepOne real-time PCR system (Applied Biosystems, Foster City, CA, USA) was used as described previously [28].…”