Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase‑1/NF‑κB signaling cascade in HMC‑1 cells

Abstract

Thymic stromal lymphopoietin (TSLP) plays an important role in allergic disorders, including atopic dermatitis and asthma. Ursolic acid (UA) has various pharmacological properties, such as antioxidant, anti-inflammatory and anticancer. However, the effect of UA on TSLP regulation has not been fully elucidated. The aim of the present study was to analyze how UA regulates the production of TSLP in the human mast cell line HMc-1. Enzyme-linked immunosorbent assay, quantitative polymerase chain reaction analysis, western blotting, caspase-1 assay and fluorescent measurements of intracellular calcium levels were conducted to analyze the regulatory effects of UA. The results revealed that UA inhibited TSLP production and mRNA expression. In addition, UA reduced the activation of nuclear factor-κB and degradation of IκBα. caspase-1 activity was increased by exposure to phorbol myristate acetate plus calcium ionophore, whereas it was reduced by UA. Finally, UA treatment prevented an increase in intracellular calcium levels. These results indicated that UA may be a useful agent for the treatment and/or prevention of atopic and inflammatory diseases, and its effects are likely mediated by TSLP downregulation.

Dexamethasone Attenuates Oncostatin M Production via Suppressing of PI3K/Akt/NF-κB Signaling in Neutrophil-like Differentiated HL-60 Cells

“…The dHL-60 cells (5 × 10 6 ) were pretreated with Dex or PBS for 1 h and stimulated with GM-CSF for 15 min (PI3K) or 30 min (Akt) or 60 min (NF-κB). Western blot analysis was conducted as previously described [ 56 , 57 , 58 ].…”

Conditioned Medium of Human Amniotic Epithelial Cells Alleviates Experimental Allergic Conjunctivitis Mainly by IL-1ra and IL-10

“…Despite previous reports on the relationship between Ca 2+ i/ NF-kB signaling and inflammatory/allergic responses (45)(46)(47), we hypothesized whether the regulatory effect of AECM on MC is mediated by NF-kB signaling. Thus, we performed Western blotting to examine NF-kB signaling and related signaling molecules.…”

Advances in Research on Chemical Constituents and Their Biological Activities of the Genus Actinidia

“…Great attention had been paid on biological activities about ursolic acid, attracting much interest in recent years. Ursolic acid exhibits different pharmacological activities, including anti-cancer, amylolytic enzyme inhibitors, cytotoxicity, downregulating thymic stromal lymphopoietin and others [7][8][9][10][11].…”

“… Part Bioactivity Refs. 1 3 β -Hydroxyurs-12-en-28-oic acid A. eriantha [ 7 ] Roots Anticancer (in vitro) [ 8 ] Inhibiting amylolytic enzyme (in vitro) [ 9 ] Antidepressant and neuroprotective (in vitro) [ 10 ] Downregulating thymic stromal lymphopoietin (in vitro) [ 11 ] 2 3 β ,24-Dihydroxyurs-12-en-28-oic acid A. arguta [ 37 ] A. polygama [ 23 ] Leaves Fruit galls 3 2 α ,3 β -Dihydroxyurs-12-en-28-oic acid A. polygama [ 23 ] A. arguta [ 13 ] Fruit galls Roots Anti-metastation (In vitro) [ 38 ] Anticancer (In vitro) [ 12 ] 4 23-Hydroxyursolic acid A. arguta [ 13 ] Roots Anti-pancreatic lipase (In vitro) [ 13 ] Anticancer(In vitro) [ 14 – 16 ] Anti-inflammatory (in vitro) [ 17 ] 5 2 β ,3 β -Dihydroxyursolic acid A. sp [ 39 ] 6 2 α ,3 α -Dihydroxyurs-12-en-28-oic acid …”