Inhibitory Effect of Mitomycin C on Proliferation of Primary Cultured Fibroblasts from Human Airway Granulation Tissues

Chen, Nan; Zhang, Jie; Xu, Min; Wang, Yuling; Pei, Ying

doi:10.1159/000346648

Cited by 16 publications

(11 citation statements)

References 21 publications

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“…4E). Mitomycin C has been shown to inhibit proliferation in a number of cell types in vitro while the cells remain viable (Chen et al, 2013;Nieto et al, 2007). We found no cardiomyocyte proliferation marked by pH3-positive cardiomyocytes treatment with mitomycin C 6 h after cryoinjury (n=3 hCOs, 1893 cells counted, Fig.…”

Section: Hcos Regenerate and Recover Cardiac Function Following Cryoimentioning

confidence: 58%

Development of a human cardiac organoid injury model reveals innate regenerative potential

et al. 2017

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The adult human heart possesses a limited regenerative potential following an ischemic event, and undergoes a number of pathological changes in response to injury. While cardiac regeneration has been documented in zebrafish and neonatal mouse hearts, it is currently unknown whether the immature human heart is capable of undergoing complete regeneration. Combined progress in pluripotent stem cell differentiation and tissue engineering has facilitated the development of human cardiac organoids (hCO), which resemble fetal heart tissue and can be used to address this important knowledge gap. This study aimed to characterise the regenerative capacity of immature human heart tissue in response to injury. Following cryoinjury with a dry ice probe, hCO exhibited an endogenous regenerative response with full functional recovery by two weeks following acute injury. Cardiac functional recovery occurred in the absence of pathological fibrosis or cardiomyocyte hypertrophy. Consistent with regenerative organisms and neonatal human hearts, there was a high basal level of cardiomyocyte proliferation, which may be responsible for the regenerative capacity of the hCO. This study suggests that immature human heart tissue has an intrinsic capacity to regenerate.

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Section: Hcos Regenerate and Recover Cardiac Function Following Cryoimentioning

confidence: 58%

Development of a human cardiac organoid injury model reveals innate regenerative potential

et al. 2017

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“…To date, few drugs have been found to effectively regulate wound healing. These include dexamethasone and mitomycin C [8]. Therefore, many clinicians have begun to focus on natural herb extracts for their curative effects and low cytotoxicity [37].…”

Section: Discussionmentioning

confidence: 99%

“…Airway stenosis patients have dyspnea and a lower quality of life [1,2,3,4]. Current treatments include surgery, drugs, and interventional bronchoscopic techniques [1,2,3,5,6,7,8,9,10]. The latter approach represents the main form of relief [3,5,6,7,10,11,12].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of β-Elemene on Human Airway Granulation Tissue in vivo and in vitro

Li¹,

Zeng

Yang

2016

Respiration

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Background: Recurrent airway granulation hyperplasia and scar formation make airway stenosis a clinical challenge. Therefore, a new approach for the treatment of airway stenosis is necessary. Objective: To explore the inhibitory effect of β-elemene on the proliferation of fibroblasts and airway granulation. Methods: In vivo: (1) study of the effect of local β-elemene injection by bronchoscopy. (2) During bronchoscopy, granulation tissues both before and after treatment were obtained. HE staining was performed and the result compared. In vitro: (1) human airway primary fibroblasts were purified and characterized. (2) Fibroblasts were treated with β-elemene and normal saline (NS) and then examined by optical and electron microscopy. (3) Fibroblasts treated with β-elemene or NS were assessed for viability by tetrazolium salt assay. (4) Apoptotic rates were determined by flow cytometry. Results: In vivo: (1) after local injection of β- elemene, airway granulation tissue was reduced. (2) Granulation tissue was found to have less edema, and fibroblasts turned into mature fiber cells. In vitro: (1) human airway primary fibroblasts were successfully purified and cultured. (2) Compared with the control group, fibroblasts of the experimental group became clumped, the plasma granules were increased, and some fibroblasts lost their nucleus and organelles. (3) Compared with the control group, reduction of cell viability was detected with increased concentrations of β-elemene. (4) With increased concentrations of β-elemene, apoptotic rates of the fibroblasts were raised compared with the control group. Conclusions: β-Elemene may induce apoptosis and necrosis of airway primary fibroblasts and inhibit the proliferation of fibroblasts and airway granulation. The results provide a new approach for the treatment of airway stenosis.

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“…Chen et al [16] collected granulation tissue from routine bronchoscopies and cultured primary fibroblasts to investigate this issue. The majority of human studies use a topical dose of 0.1-0.4 mg/ml with the majority of animal studies using 2-10 mg/ml.…”

Section: Discussionmentioning

confidence: 99%

The use of mitomycin C in pediatric airway surgery

Gangar

Bent

2014

Current Opinion in Otolaryngology & Head and Neck Surgery

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Inhibitory Effect of Mitomycin C on Proliferation of Primary Cultured Fibroblasts from Human Airway Granulation Tissues

Cited by 16 publications

References 21 publications

Development of a human cardiac organoid injury model reveals innate regenerative potential

Development of a human cardiac organoid injury model reveals innate regenerative potential

Inhibitory Effect of β-Elemene on Human Airway Granulation Tissue in vivo and in vitro

The use of mitomycin C in pediatric airway surgery

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