Inhibitory effect of intravenous lysine infusion on urea cycle metabolism

Kato, Tomoaki; Sano, M.; Mizutani, Naoki

doi:10.1007/bf00647285

Cited by 28 publications

(8 citation statements)

References 18 publications

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“…Lysine may affect catabolism, as shown by the severe increase in BUN, CRN, and ammonia from Day 1. It was reported that lysine induced an inhibitory effect on the urea cycle in normal volunteer subjects (Kato et al, 1987). Lysine is known to be a powerful competitive antagonist to arginine (O'Dell and Savage, 1966;Kato et al, 1987), to inhibit liver arginase activity (Cittadini et al, 1964), and to increase the liver arginine concentration (Statter and Russell, 1978).…”

Section: Discussionmentioning

confidence: 99%

Effects of Lysine-Induced Acute Renal Failure in Dogs

et al. 2006

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-This study investigates the effects of lysine-induced acute renal failure. Female dogs received a lysine hydrochloride (lysine) of 4500 mg/kg/day (3.75 ml/kg/hr) for 3 consecutive days. The dogs were observed for clinical signs. Body weights were recorded, food consumption and water consumption calculated, and urinalysis and blood biochemistry were performed daily. Plasma samples for amino acid determinations were obtained from all dogs, which were necropsied on Day 3. Histopathological examinations were done on all test animals. Compound-related findings include the following. Blood biochemistry results showed increases in ammonia, blood urea nitrogen, blood urea nitrogen/creatinine ratio, and creatinine. Urinary changes consisted of increases in urine volume, total protein, albumin, γ-glutamyl transpeptidase, and N-acetyl-β-D-glucosaminidase. In addition, macroscopic findings consisted of pale, congested capsule; microscopic findings consisted of hypertrophy of proximal convoluted tubule (mainly S1 segment), and degeneration/desquamation of urinary tubule (mainly S3 segment with hyaline casts) in the kidney. From these findings, it can be concluded that lysine is nephrotoxic in dogs. Nephrotoxicity of lysine may relate to direct tubular toxicity and to tubular obstruction.

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Section: Discussionmentioning

confidence: 99%

Effects of Lysine-Induced Acute Renal Failure in Dogs

et al. 2006

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“…In addition, lysine is a potent inhibitor of arginase and hence blocks arginase-mediated urea synthesis from arginine (Egan et al, 1995). Intravenous infusion of lysine resulted in a significant increase in plasma levels of arginine (Kato et al, 1987), suggesting increased availability of arginine for NO synthesis. In general, lysine signaling might play a number of important physiological functions.…”

Section: Introductionmentioning

confidence: 99%

Lysine and arginine reduce the effects of cerebral ischemic insults and inhibit glutamate-induced neuronal activity in rats

Kondoh¹

2010

Front. Integr. Neurosci.

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Intravenous administration of arginine was shown to be protective against cerebral ischemic insults via nitric oxide production and possibly via additional mechanisms. The present study aimed at evaluating the neuroprotective effects of oral administration of lysine (a basic amino acid), arginine, and their combination on ischemic insults (cerebral edema and infarction) and hemispheric brain swelling induced by transient middle cerebral artery occlusion/reperfusion in rats. Magnetic resonance imaging and 2,3,5-triphenyltetrazolium chloride staining were performed 2 days after ischemia induction. In control animals, the major edematous areas were observed in the cerebral cortex and striatum. The volumes associated with cortical edema were significantly reduced by lysine (2.0 g/kg), arginine (0.6 g/kg), or their combined administration (0.6 g/kg each). Protective effects of these amino acids on infarction were comparable to the inhibitory effects on edema formation. Interestingly, these amino acids, even at low dose (0.6 g/kg), were effective to reduce hemispheric brain swelling. Additionally, the effects of in vivo microiontophoretic (juxtaneuronal) applications of these amino acids on glutamate-evoked neuronal activity in the ventromedial hypothalamus were investigated in awake rats. Glutamate-induced neuronal activity was robustly inhibited by microiontophoretic applications of lysine or arginine onto neuronal membranes. Taken together, our results demonstrate the neuroprotective effects of oral ingestion of lysine and arginine against ischemic insults (cerebral edema and infarction), especially in the cerebral cortex, and suggest that suppression of glutamate-induced neuronal activity might be the primary mechanism associated with these neuroprotective effects.

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“…In an other study [38] , 0.5 mmol/kg of L-lysine was injected to children between 6 to 14 years of age; and then, increases in plasma ornithine and arginine levels and urine ammonia concentration were detected. This case was explained with the inhibition of the enzyme arginase.…”

Section: Discussionmentioning

confidence: 99%

L-Kanavanin ve L-Lizin’in Koyun Dalak Doku Arginaz Aktivitesi Üzerine İnhibisyon Etkisi

Kandemir¹,

Kaymaz²,

Özdemir³

2014

Kafkas Univ Vet Fak Derg

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Arginase (L-arginine amidinohydrolase; (E.C.3.5.3.1) is the last enzyme of urea cycle and it catalyzes the hydrolysis of L-arginine to urea and L-ornithine in livers of animals. It helps to the excretion process of urea from the body, which is the most soluble and non-toxic form of nitrogen. Along with its essential role in urea synthesis, arginase is also found to have important roles in ornithine production for polyamine, proline and glutamate synthesis and in immune system activation. Mostly found in liver tissue, arginase enzyme is also found in non-ureolytic tissues like heart, rumen, skeletal muscle, kidney, intestine, brain, spleen, thyroid gland, salivary gland, erythrocyte, fibroblast, macrophage, mammary gland in lactation, testicle. With this study, it was aimed to assess the inhibitory effects of arginase in sheep spleen tissue on L-canavanine and L-lysine. Consequently, it was observed that L-lysine as a L-amino acid and guanidine compound L-canavanine inhibits enzyme activity non-competitively. Keywords: Arginase, L-lysine, L-canavanine, Sheep spleen tissue L-Kanavanin ve L-Lizin'in Koyun Dalak Doku Arginaz Aktivitesi Üzerine İnhibisyon EtkisiÖzet Arginaz (L-arginin amidino hidrolaz; (E.C.3.5.3.1) üre döngüsünün son enzimi olup hayvanların karaciğerlerindeki L-arginini, üreye L-ornitine hidrolizini katalizeleyen bir enzimdir. Azotun en iyi çözünebilir ve nontoksik formu olan üre oluşumuna katkıda bulunarak vücuttan atılmasına yardımcı olur. Arginaz üre sentezindeki vazgeçilmez rolünün yanında; poliamin, prolin ve glutamat sentezi için ornitin üretimini sağlayıp, ayrıca immün sistem aktivasyonunda ve tümör biyolojisinde de rol oynadığı saptanmıştır. Arginaz enzimi en çok karaciğer dokusunda bulunmakla beraber; kalp, rumen, iskelet kası, böbrek, bağırsak, beyin, dalak, tiroit bezi, tükürük bezi, eritrosit, fibrobast, makrofaj, laktasyondaki meme bezi, testis gibi nonüreolitik dokularda da bulunmaktadır. Bu çalışma ile koyun dalak doku arginazı üzerine L-kanavanin ve L-lizinin inhibisyon etkileri incelenmesi amaçlanmıştır. Sonuç olarak, bir L-amino asit olan L-lizin ve guanidin bileşiklerinden L-kanavaninin enzimi non-kompetetif olarak inhibe ettiği görüldü.

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Inhibitory effect of intravenous lysine infusion on urea cycle metabolism

Cited by 28 publications

References 18 publications

Effects of Lysine-Induced Acute Renal Failure in Dogs

Effects of Lysine-Induced Acute Renal Failure in Dogs

Lysine and arginine reduce the effects of cerebral ischemic insults and inhibit glutamate-induced neuronal activity in rats

L-Kanavanin ve L-Lizin’in Koyun Dalak Doku Arginaz Aktivitesi Üzerine İnhibisyon Etkisi

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