Inhibitory effect of herbal medicines and their trapping abilities against methylglyoxal-derived advanced glycation end-products

Sompong, Weerachat

doi:10.1186/s12906-015-0897-8

Cited by 29 publications

(21 citation statements)

References 48 publications

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“…The total phenolic content (TPC) of extracts was previously reported by our experiments [15] that have been used for the calculation of Pearson’s correlation coefficients in the present study. The Pearson’s correlation coefficients between the variables are presented in Table 5.…”

Section: Resultsmentioning

confidence: 99%

“…The plant authentication was performed according to our previous report [15]. The extraction of herbal medicines was done according to a previous report [15]. Briefly, the plants (20 g) were boiled in distilled water (800 mL) for 3 h at 95 °C before filtered through Whatman No.…”

Section: Methodsmentioning

confidence: 99%

“…As described in Table 1, herbal medicines have been commonly used for the treatment of various diseases in the Ayurvedic system of Thai traditional medicine. Previous reports have shown the methylglyoxal-trapping abilities of herbal medicines and their inhibitory activities on the formation of protein glycation [14, 15]. Nevertheless, studies addressing the inhibition of these herbal medicines on the key enzymes and steps of carbohydrate and lipid digestions remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Sompong

Muangngam

Kongpatpharnich

et al. 2016

BMC Complement Altern Med

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BackgroundObesity and overweight are consistently associated with metabolic disorders including hyperglycemia and hyperlipidemia. Herbal medicines have been currently suggested as an alternative source of potentially useful antihyperglycemic, antihyperlipidemic, antioxidant activities. The objective of this study was to assess the in vitro inhibitory effects of eleven herbal medicines on carbohydrate and lipid digestive enzymes and the key steps of lipid digestion including the inhibition of micelle formation and the ability to bind bile acid. In addition, antioxidant activity of herbal medicines was also investigated.Methodsα-Glucosidase, pancreatic α-amylase, pancreatic lipase, and pancreatic cholesterol esterase inhibitory activities of aqueous extract of herbal medicines were measured using the enzymatic colorimetric assay. The formation of cholesterol micelles was determined using the cholesterol assay kit. The bile acid binding was measured using the colorimetric assay. Antioxidant activities were assessed by using four methods including Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity ORAC), superoxide radical scavenging activity (SRSA), and hydroxyl radical scavenging activity (HRSA).ResultsThe extracts (1 mg/mL) markedly inhibited intestinal maltase (5.16 − 44.33 %), sucrase (1.25–45.86 %), pancreatic α-amylase (1.75–12.53 %), pancreatic lipase (21.42–85.93 %), and pancreatic cholesterol esterase (2.92–53.35 %). The results showed that all extracts exhibited the inhibitory activity against pancreatic lipase with the IC50 values ranging from 0.015 to 4.259 mg/mL. In addition, the incorporation of cholesterol into micelles was inhibited by the extracts (6.64–33.74 %). The extracts also bound glycodeoxycholic acid (9.9–15.08 %), taurodeoxycholic acid (12.55–18.18 %), and taurocholic acid (11.91 − 18.4 %). Furthermore, the extracts possessed various antioxidant activities including the TEAC values (0.50 − 4.70 μmol trolox/mg dried extract), the ORAC values (9.14–44.41 μmol trolox/mg dried extract), the SRSA (0.31 − 18.82 mg trolox/mg dried extract), and the HRSA (0.05–2.29 mg trolox/mg dried extract). The findings indicated that Syzygium aromaticum, Phyllanthus amarus, Thunbergia laurifolia were the effective promising antihyperglycemic and antihyperlipidemic agents. Statistical analysis demonstrated strong positive significant correlations between the contents of phenolic compounds and % inhibition of pancreatic lipase (r = 0.885, p < 0.001), % inhibition of pancreatic cholesterol esterase (r = 0.761, p < 0.001), and the TEAC value (r = 0.840, p < 0.001). Furthermore, there was a strongly positive correlation between the TEAC value and % inhibition of pancreatic cholesterol esterase (r = 0.851, p < 0.001) and % inhibition of pancreatic lipase (r = 0.755, p < 0.001).ConclusionsThree herbal medicines including Syzygium aromaticum, Thunbergia laurifolia, and Phyllanthus amarus markedly inhibited the intestinal α-glucosidase, pancreatic α-amylase, pancreatic lipase, and pancreat...

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Sompong

Muangngam

Kongpatpharnich

et al. 2016

BMC Complement Altern Med

Self Cite

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“…Many natural products, including the extracts of herbal plants, mung beans, peanuts, or natural compounds, such as quercetin and myricitrin, have been reported to inhibit the formation of AGEs induced by MGO [21,[32][33][34][35]. Moreover, it has been reported that natural products block the MGO-induced AGE formation through the trapping of MGO [21,32]. This MGO trapping mechanism by flavonoids from natural products involves the trapping of the reactive dicarbonyl MGO species at the two unsubstituted positions 6 and 8 in the A-ring, the major active sites of flavonoids.…”

Section: Discussionmentioning

confidence: 99%

Psoralea corylifoliaL. Seed Extract Attenuates Methylglyoxal-Induced Insulin Resistance by Inhibition of Advanced Glycation End Product Formation

Truong

Seo

Jun

2019

Oxidative Medicine and Cellular Longevity

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Accumulation of advanced glycation end products (AGEs) in the body has been implicated in the pathogenesis of metabolic conditions, such as diabetes mellitus. Methylglyoxal (MGO), a major precursor of AGEs, has been reported to induce insulin resistance in both in vitro and in vivo studies. Psoralea corylifolia seeds (PCS) have been used as a traditional medicine for several diseases, but their potential application in treating insulin resistance has not yet been evaluated. This study is aimed at investigating whether PCS extract could attenuate insulin resistance induced by MGO. Male C57BL/6N mice (6 weeks old) were administered 1% MGO in their drinking water for 18 weeks, and the PCS extract (200 or 500 mg/kg) was orally administered daily from the first day of the MGO administration. We observed that both 200 and 500 mg/kg PCS extract treatment significantly improved glucose tolerance and insulin sensitivity and markedly restored p-Akt and p-IRS1/2 expression in the livers of the MGO-administered mice. Additionally, the PCS extract significantly increased the phosphorylation of Akt and IRS-1/2 and glucose uptake in MGO-treated HepG2 cells. Further studies showed that the PCS extract inhibited MGO-induced AGE formation in the HepG2 cells and in the sera of MGO-administered mice. PCS extract also increased the expression of glyoxalase 1 (GLO1) in the liver tissue of MGO-administered mice. The PCS extract significantly decreased the phosphorylation of ERK, p38, and NF-κB and suppressed the mRNA expression of proinflammatory molecules including TNF-α and IL-1β and iNOS in MGO-administered mice. Additionally, we demonstrated that the PCS extract attenuated oxidative stress, as evidenced by the reduced ROS production in the MGO-treated cells and the enhanced expression of antioxidant enzymes in the liver of MGO-administered mice. Thus, PCS extract ameliorated the MGO-induced insulin resistance in HepG2 cells and in mice by reducing oxidative stress via the inhibition of AGE formation. These findings suggest the potential of PCS extract as a candidate for the prevention and treatment of insulin resistance.

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“…Some of these exhibited antidiabetic activity in previous studies. They have also been reported to possess other pharmacological effects, such as antimicrobial, 24,25) antiviral, 26) anticancer, 27) anti-inflammatory, 28,29) antipyretic, 29) analgesic, 29) antioxidant, 30) antihemolytic, 31) immunomodulatory, 32) antiallergic, 33) neuroprotective, 34,35) antiglycation, 36) and antiobesity 37) activities (Table 2).…”

Section: Naphthoquinones Containing Plants In Thailandmentioning

confidence: 99%

Antidiabetic Naphthoquinones and Their Plant Resources in Thailand

Shah

Keach²,

Panichayupakaranant

2018

Chem. Pharm. Bull.

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Diabetes mellitus is the seventh leading cause of death globally. Ninety percent of the diabetic population suffers from type-2 diabetes, which still needs an effective, safe and economical oral hypoglycemic therapy. Plants are rich sources of various therapeutic molecules. More than 400 medicinal plants of interesting phytochemical diversity have been reported for their antidiabetic potential. Naphthoquinones are a group of phytochemicals, which have a wide range of pharmacological potential, including antidiabetic activity. Naphthoquinones exert their antidiabetic effects through various mechanisms such as the inhibition of α-glucosidase and protein tyrosine phosphatase 1B, increased glucose uptake in myocytes and adipocytes via glucose transporter type 4 (GLUT4) and GLUT2 translocations, enhanced peroxisome proliferator-activated receptor gamma (PPARγ) ligand activity, and by normalizing carbohydrate metabolizing enzymes in the liver. Moreover, naphthoquinone inhibits adipogenesis by both upstream and downstream regulation to control obesity, which is one of the important risk factors for diabetes. Naturally occurring naphthoquinones, as well as their plant sources, are therefore of interest for exploring their antidiabetic potential. The present review aims to overview the antidiabetic potential of naphthoquinones and their plant resources in Thailand.

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Inhibitory effect of herbal medicines and their trapping abilities against methylglyoxal-derived advanced glycation end-products

Cited by 29 publications

References 48 publications

The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Psoralea corylifoliaL. Seed Extract Attenuates Methylglyoxal-Induced Insulin Resistance by Inhibition of Advanced Glycation End Product Formation

Antidiabetic Naphthoquinones and Their Plant Resources in Thailand

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