Inhibitory Effect of Coumarins from Weigela subsessilis on Low Density Lipoprotein Oxidation

Thương, Phương Thiện; Na, MinKyun; Su, Nguyen Duy; Seong, Rack Seon; Lee, Young Mi; Sok, Dai‐Eun; Bae, KiHwan

doi:10.1248/bpb.28.1095

Cited by 23 publications

(17 citation statements)

References 17 publications

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“…Blood was collected into EDTA tubes, and human LDL was prepared from plasma by sequential flotation ultracentrifugation as previously described. 12,20) For Cu 2ϩ -mediated oxidation experiments, LDL was dialyzed for 20 h at 4°C against EDTA-free, phosphate-buffered saline (PBS, pH 7.4) to remove EDTA. For azo-initiated oxidation experiments, LDL was dialyzed overnight against the same PBS containing 1 mM EDTA.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Despite numerous studies on the inhibitory activities of natural antioxidants against LDL oxidation, reports on the effects of coumarinoids are still scarce. 12) Lee et al have shown that esculetin inhibits LDL oxidation and Apo B fragmentation. 13) In this study, we compared the protective effects of esculetin and fraxetin on LDL oxidation induced by a metal ion (Cu 2ϩ ).…”

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confidence: 99%

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Dual Anti-oxidative Effects of Fraxetin Isolated from Fraxinus rhinchophylla

Thương

Pokharel

Lee

et al. 2009

Biological & Pharmaceutical Bulletin

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Atherosclerosis is main cause of arteriosclerosis. The pivotal role of low-density lipoprotein (LDL) oxidation in atherogenesis suggests antioxidants may help prevent cardiovascular disease. Fraxinus rhynchophylla DENCE (Oleaceae) is a traditional medicinal plant from East Asia. During the course of characterizing potential drug candidates from natural products, we isolated two major coumarins, esculetin and fraxetin and found that fraxetin has dual-antioxidative functions. Low concentrations (1-5 m mM) of fraxetin potently inhibited LDL oxidation induced by metal and free radicals. Moreover, treatment of vascular smooth muscle cells (VSMCs) with higher concentrations (above 30 m mM) of fraxetin significantly increased the protein level of heme oxygenase-1 (HO-1), a key enzyme that inhibits vascular proliferation and atherosclerosis. Subcellular fractionation and reporter gene analysis using an antioxidant response element (ARE) construct revealed that fraxetin increased the level of nuclear factor (NF)-E2-related factor 2 (Nrf2) and reporter activity, and these were associated with the induction of antioxidant enzymes, such as HO-1 and glutathione S-transferase-a a. In conclusion, fraxetin has direct protective properties against LDL oxidation at lower concentrations, and higher concentrations of fraxetin induce antioxidant enzymes via Nrf2/ARE activation. These effects suggest potential anti-atherosclerosis effects of Fraxinus rhynchophylla D.

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Section: Plant Materialsmentioning

confidence: 99%

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confidence: 99%

Dual Anti-oxidative Effects of Fraxetin Isolated from Fraxinus rhinchophylla

Thương

Pokharel

Lee

et al. 2009

Biological & Pharmaceutical Bulletin

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“…16) With regard to the chemical constituents, flavonoids and coumarins have been isolated from the leaf and flower of this edible plant. [17][18][19] Our phytochemical study of the MeOH extract of the leaf and stem of this plant resulted in the isolation of a new and seven known ursane-type triterpenoids. Accordingly, although the anti-complement activities of oleanene-type, [9][10][11][12][13] dammarane-type, 14) and lanostane-type 15) have been well documented, little is known whether ursane-type triterpenoids are potent anti-complement compounds.…”

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Anti-complementary Activity of Ursane-Type Triterpenoids from Weigela subsessilis

Thương

Min

Jin

et al. 2006

Biological & Pharmaceutical Bulletin

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A new ursane-type triterpenoid, weigelic acid (1), and seven known compounds, ursolic acid (2), ilekudinol A (3), corosolic acid (4), ilekudinol B (5), esculentic acid (6), pomolic acid (7), and asiatic acid (8) were isolated from the leaf and stem of Weigela subsessilis. The structure of the new triterpenoid was established as 1b b,2a a,3a a,23-tetrahydroxyurs-12-en-28-oic acid on the basis of spectroscopic analyses. In addition, the isolated compounds were evaluated for their anti-complement activity against the classical pathway of the complement system. Of these, compounds 1-2 and 4-8 exhibited anti-complement activity with IC 50 values of 152, 90, 130, 51, 56, 4, and 163 m mM, respectively, whereas 3 was inactive. This shows that a carboxylic group of ursane-type triterpenoids seems to play an important role in inhibiting the hemolytic activity of human serum against erythrocytes.

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“…Scopoletin was only detected in S. excelsus at 3.41 mg/g extract, whereas quercetin was not detected in both plants. Scopolin and its aglycone scopoletin are coumarins that were previously isolated from the flowers of S. excelsus and W. subsessilis [10,11]. These coumarins exhibit anti-inflammatory effects, and inhibit different enzymes, including nitric oxide synthase, prostaglandin synthase, monoamine synthase, and acetylcholinesterase [23,24].…”

Section: Resultsmentioning

confidence: 99%

“…Recent studies have shown that extracts from these plants are rich in bioactive compounds. Particularly, scopoletin and scopolin are coumarins isolated from the flowers of both these plants with various biological activities [10,11]. However, bioactive properties of S. excelsus and W. subsessilis are not fully explored.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects ofSynurus excelsusandWeigela subsessilison aldose reductase and HPLC-UV analysis of scopolin, scopoletin, and quercetin

Quilantang

Lee

Ryu

et al. 2018

JABC

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The inhibition of aldose reductase (AR) has been shown to prevent the progression of the many complications associated with diabetic hyperglycemia. Several compounds purified from various plant sources have exhibited potent inhibition against AR. In this study, the inhibitory effects of the methanol extracts of the flowers of Synurus excelsus and Weigela subsessilis on AR were determined in vitro. Scopolin and scopoletin are coumarins isolated from the flowers of S. excelsus and W. subsessilis; and quercetin is a known AR inhibitor present in many flowers. To determine and quantify their presence in both plants, HPLC-UV analysis of all three compounds was performed. S. excelsus and W. subsessilis showed potent inhibition against AR having IC 50 values of 0.17 and 0.14 μg/mL , respectively. The concentration of scopolin in S. excelsus and W. subsessilis were 34.71 and 174.14 mg/g extract, respectively. Scopoletin was detected in S. excelsus at 3.41 mg/g extract, whereas quercetin was not detected in both plants. This study shows that S. excelsus and W. subsessilis exhibited promising AR inhibitory effects and are both sources of coumarins.

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Inhibitory Effect of Coumarins from Weigela subsessilis on Low Density Lipoprotein Oxidation

Cited by 23 publications

References 17 publications

Dual Anti-oxidative Effects of Fraxetin Isolated from Fraxinus rhinchophylla

Dual Anti-oxidative Effects of Fraxetin Isolated from Fraxinus rhinchophylla

Anti-complementary Activity of Ursane-Type Triterpenoids from Weigela subsessilis

Inhibitory effects ofSynurus excelsusandWeigela subsessilison aldose reductase and HPLC-UV analysis of scopolin, scopoletin, and quercetin

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