Dual Anti-oxidative Effects of Fraxetin Isolated from Fraxinus rhinchophylla

Thương, Phương Thiện; Pokharel, Yuba Raj; Lee, Moo Yeol; Kim, Sang Kyum; Bae, KiHwan; Su, Nguyen Duy; Oh, Won Keun; Kang, Keon Wook

doi:10.1248/bpb.32.1527

Cited by 58 publications

(43 citation statements)

References 45 publications

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“…However, we found that nuclear translocation of p65, the active subunit of NF-kB, was not altered by 30 mM trans-resveratrol (30 mM is the most active concentration for the induction of HO-1 by trans-resveratrol in rat VSMCs). Because transfection efficiency in rat VSMC is extremely low, NF-kB-dependent reporter activity was performed using MEF cells [28]. NF-kB reporter activity was not significantly changed in MEF cells treated with 3-30 mM trans-resveratrol (Fig.…”

Section: Nrf2/are Activation Is Required For Ho-1 Induction By Trans-mentioning

confidence: 99%

Inhibition of neointimal formation by trans‐resveratrol: Role of phosphatidyl inositol 3‐kinase‐dependent Nrf2 activation in heme oxygenase‐1 induction

Kim

Lim

Lee

et al. 2010

Molecular Nutrition Food Res

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Neointima, defined as abnormal growth of the intimal layer of blood vessels, is believed to be a critical event in the development of vascular occlusive disease. Although resveratrol's inhibitory effects on proliferation and migration of vascular smooth muscle cells has been reported, its activity on neointimal formation is still unclear. Oral administration of trans-resveratrol significantly suppressed intimal hyperplasia in a wire-injured femoral artery mouse model. In cultured vascular smooth muscle cells, trans-resveratrol inhibited platelet-derived growth factor-stimulated DNA synthesis and cell proliferation with down-regulation of cyclin D and pRB. Moreover, platelet-derived growth factor-induced production of reactive oxygen species was inhibited by trans-resveratrol and the compound induced heme oxygenase-1 (HO-1). The anti-proliferative activity of trans-resveratrol was reversed by an HO-1 inhibitor, ZnPPIX. Subcellular fractionation and reporter gene analyses revealed that trans-resveratrol increased the level of nuclear Nrf2 and antioxidant response element reporter activity, and that these were essential for the induction of HO-1. Trans-resveratrol also enhanced the activities of phosphatidyl inositol 3-kinase and extracellular signal regulated kinase, and phosphatidyl inositol 3-kinase was required for Nrf2/antioxidant response element-dependent HO-1 induction. These data have significant implications for the elucidation of the pharmacological mechanism by which trans-resveratrol prevents vascular occlusive diseases.

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Section: Nrf2/are Activation Is Required For Ho-1 Induction By Trans-mentioning

confidence: 99%

Inhibition of neointimal formation by trans‐resveratrol: Role of phosphatidyl inositol 3‐kinase‐dependent Nrf2 activation in heme oxygenase‐1 induction

Kim

Lim

Lee

et al. 2010

Molecular Nutrition Food Res

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“…The compound fraxetin was also identified in a related species Jatropha podagrica 19 . Fraxetin was also detected in Fraxinus rhinchophylla, Cortex fraxini and Lawsonia inermis and are proven as antioxidants [20][21][22] . Fraxetin is an antihyperglecemic compound, which prevents rotenone-induced apoptosis by induction of endogenous glutathione in human neuroblastoma cells 23 .…”

Section: Fig3: Antioxidant Activity Of Different Solvent Extracts Ofmentioning

confidence: 98%

Untitled

2016

IJPSR

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Since, the free radicals induced oxidative stress is posing threat present researchers are focusing on natural sources that could serve as antioxidants to combat the oxidative damage mediated diseases such as diabetes, atherosclerosis and cancer. In this context, present study was carried out to investigate the botanical characters, chemical composition and in vitro antioxidant potential of Jatropha gossypifolia L. so as to provide scientific evidences for the medicinal properties claimed on this plant. Microscopic studies were conducted on both entire leaf and leaf powder, the elemental composition of leaf powder was investigated. Extracts of J. gossypifolia leaf was prepared with hexane, chloroform, ethyl acetate, ethanol and water and screened for the detection of various phytochemicals and the major phytochemical compounds present were identified through HPTLC and GC-MS analysis. In vitro antioxidant activity of solvent extracts was evaluated through reducing power and DPPH radical scavenging assays. Phytochemical screening revealed the presence of phenols, flavones, glycosides, steroids, alkaloids and quinines in the ethanolic extract. Quantification of phytochemicals showed 3.35 mg/kg of alkaloids, 3.60 mg/kg flavonoids, 0.22 mg/kg tannins and 0.12 mg/kg glycosides. Elemental composition revealed the existence of high level of calcium (3.89%) and iron (85 ppm), which have clinical importance. Presence of alkaloids, quercetin, and fraxetin were confirmed with HPTLC analysis. Among the different solvent extracts, ethyl acetate exhibited higher antioxidant activity. Presence of phytochemicals such as p-Cymene, Fumaric acid, 12-Octadecanoic acid, 1-Heptadecanol, and Yashabushiketol were confirmed in this plant through GC-MS analysis. Botanical and chemical standards were determined for J. gossypifolia leaf through the present investigation and the results of in vitro antioxidant assays gives the scientific evidence for the health benefits and medicinal effects claimed on J. gossypifolia.

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“…Aesculin, a 6, 7-dihydroxycoumarin-6-O-D-β-glucopyranoside, is a coumarin glucoside naturally found in stem barks of Fraxinus rhynchophyllycosidea and widely used for the treatment of diarrhea, leukorrhea, and inflammatory diseases such as arthritis and gout [14,18,19]. The anti-inflammatory activity of aesculin is attributed to activating nuclear factor-E2-related factor 2 (Nrf2) as well as inhibiting NF-κB [5,15].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Glycosyl Aesculin Synthesis by Thermotoga neapolitana β-Glucosidase Using Response-surface Methodology

Park¹,

Park²,

Cha³

2017

Journal of Life Science

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Glycosyl aesculin, a potent anti-inflammatory agent, was synthesized by transglycosylation reaction, catalyzed by Thermotoga neapolitana β-glucosidase, with aesculin as an acceptor. The key reaction parameters were optimized using response-surface methodology (RSM) and 2 μg of the enzyme. As shown by a statistical analysis, a second-order polynomial model fitted well to the data (p<0.05). The response surface curve for the interaction between aesculin and other parameters revealed that the aesculin concentration and reaction time were the primary factors that affected the yield of glycosyl aesculin. Among the tested factors, the optimum values for glycosyl aesculin production were as follows: aesculin concentration of 9.5 g/l, temperature of 84℃, reaction time of 81 min, and pH of 8.2. Under these conditions, 61.7% of glycosyl aesculin was obtained, with a predicted yield of 5.86 g/l.The maximum amount of glycosyl aesculin was 6.02 g/l. Good agreement between the predicted and experimental results confirmed the validity of the RSM. The optimization of reaction conditions by RSM resulted in a 1.6-fold increase in the production of glycosyl aesculin as compared to the yield before optimization. These results indicate that RSM can be effectively used for process optimization in the synthesis of a variety of biologically active glycosides using bacterial glycosidases.

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Dual Anti-oxidative Effects of Fraxetin Isolated from Fraxinus rhinchophylla

Cited by 58 publications

References 45 publications

Inhibition of neointimal formation by trans‐resveratrol: Role of phosphatidyl inositol 3‐kinase‐dependent Nrf2 activation in heme oxygenase‐1 induction

Inhibition of neointimal formation by trans‐resveratrol: Role of phosphatidyl inositol 3‐kinase‐dependent Nrf2 activation in heme oxygenase‐1 induction

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Optimization of Glycosyl Aesculin Synthesis by Thermotoga neapolitana β-Glucosidase Using Response-surface Methodology

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