Inhibitory Effect of Coffee on Hepatoma Proliferation and Invasion in Culture and on Tumor Growth, Metastasis and Abnormal Lipoprotein Profiles in Hepatoma-Bearing Rats

Abstract

Summarywe have already reported that instant coffee powder (ICP) and ICP4oaded rat sera could suppress proliferation and invasion of rat ascites hepatoma cell line of AH109A in vitro. In this report, we examined the mechanisms for suppression of tumor cell prolifer ation and invasion by ICP, and the effect of ICP on in vivo tumor growth, metastasis and abnormal lipoprotein profiles in hepatoma-bearing rats. ICP, when directly added to the cuh ture media, induced cell cycle arrest (elongation of S phase) at a lower concentration (0.3 mg/mL) and apoptosis at a higher concentration (0.6-1.2mg/mL). ICP and ICP-loaded rat sera showed reactive oxygen species (OS)-scavenging property and canceled the enhance ment of invasive activity of hepatoma cells induced by OS in vitro. These results suggest that ICP suppresses the proliferation by inducing cell cycle arrest and apoptosis, and the invasion by scavenging ROS and that ICP could retain these properties after their gas trointestinal absorption. The hepatoma-bearing rats were fed with a 20% casein diet (20C) or 20C supplemented with 0.1% ICP for 14 d. Dietary ICP significantly reduced solid tumor growth and tended to reduce hepatoma metastases to lung and lymphatic nodes, suggesting that ICP could suppress tumor cell proliferation and invasion in vivo. In addition, dietary ICP significantly increased serum high density lipoprotein (HDL)-cholesterol and tended to reduce very low-density and low-density lipoprotein (ULDL+LDL)-cholesterol, resulting in amelioration of abnormal lipoprotein profiles occurred in hepatoma-bearing rats. In conclu sion, ICP has the ability to induce cell cycle arrest and apoptosis in hepatoma cells and to suppress tumor cell invasion by reducing oxidative stresses in vitro, and it could also exhibit these effects in vivo, leading to the inhibition of tumor growth and metastases.

Lipids changes in liver cancer

“…Drinking instant coffee powder results in amelioration of abnormal lipoprotein profiles that occurred in hepatoma-bearing rats, and instant coffee powder has the ability to suppress tumor cell invasion by reducing oxidative stresses in vitro, leading to the inhibition of tumor growth and metastases (Miura et al, 2004). According to the metabolic characteristic of lipoids and lipoprotein (Chu et al, 2001;Kader et al, 1998), high-density lipoprotein has been used as a potential carrier for delivery of a lipophilic antitumoral drug into hepatoma cells (Lou et al, 2005).…”

Anti-Invasive Activity of Niacin and Trigonelline against Cancer Cells

“…Coffee reportedly suppresses hepatoma cell invasion by reducing oxidative stress in vitro. 9) Thus it is unlikely that trigonelline is responsible for the inhibitory action of coffee on hepatoma invasion by way of scavenging ROS. Other components such as chlorogenic acid and caffeic acid 18) might be responsible for the suppressive effect of coffee on invasion through reduction of oxidative stress.…”

“…[5][6][7][8] Coffee is reported to suppress tumor cell invasion by reducing oxidative stresses in vitro and ex vivo. 9) Trigonelline is a major constituent in coffee and niacin-related compound. Niacin is one of the watersoluble vitamins, and reportedly has moderate radicalscavenging activity.…”

Tea, Coffee and Prostate Cancer

“…However, the majority of research has reported a wide range of chemopreventive effects [45,46]. Recent in vitro and in vivo studies in rats have also shown that coffee can suppress proliferation and invasion, possibly by inducing cell cycle arrest, apoptosis and the invasion by scavenging ROS, and can ameliorate abnormal lipoprotein profiles [50].…”