Inhibitory effect of circulating fibrocytes on injury repair in acute lung injury/acute respiratory distress syndrome mice model

Abstract

The study was aimed to explore the functions of circulating fibrocytes (CFs) on injury repair in acute lung injury/acute respiratory distress syndrome (ALI/ARDS) mice model and its clinical value as a biomarker for ALI/ARDS. ALI/ARDS mice model was established by intratracheal instillation of lipopolysaccharide (LPS). Mononuclear cells were isolated from peripheral blood of ALI/ARDS model and flow cytometry was used to measure CFs defined as cells positive for CD45 and collagen-1. Histological changes of lung tissues were evaluated by H&E staining and Masson's trichrome staining. The correlations of CFs counts with damnification of lung tissue and the severity of pulmonary fibrosis were evaluated by Pearson correlation analyses. Western blot was used to detect the protein expression of collagen-1. ELISA was applied to determine cytokine CXCL12 concentration. Clinical relevance between CFs and ALI/ARDS was investigated. The greater number of CFs in the ALI/ARDS group implied higher degree of lung injury and more severe pulmonary fibrosis. The protein expression of collagen-1 and concentration of cytokine CXCL12 in ALI/ARDS group were higher than that in control group. Clinical and prognostic analysis revealed the higher injury degree and death rates in ALI/ARDS group than those in control group, and identified a greater severity and mortality for patients with ARDS than those with ALI. ROC curve analysis indicated the counts of CFs greater than 5.85% can predict death rates with AUC = 0.928. CFs had an inhibitory effect on injury repair in ALI/ARDS mice model. This might be unfavorable as a clinical marker for progression of ALI/ARDS.

Circulating fibrocytes traffic to the lung in murine acute lung injury and predict outcomes in human acute respiratory distress syndrome: a pilot study

“…2a and 3a). Interestingly, elevated percentage of circulating fibrocytes at a single time point has been correlated with increased mortality in patients with ARDS (Tai et al 2018). The present work adds to this literature by showing that subjects with ARDS develop episodic marked elevations in circulating fibrocytes that were predictive of outcomes, similar to patients with fibrotic interstitial lung diseases (Thille et al 2013;Moeller et al 2009).…”

Quantification of circulating tumour cells over time in dogs with appendicular osteosarcoma

“…In order to reproducibly enumerate rare CTC (<0.001%) among mixed leukocytes, and possibly a few endothelial cells, fibrocytes, macrophages and other cells, it was necessary to gather light scatter and fluorescence properties of >10 6 total cells. In humans, wound and fracture repair, and fibroproliferative disorders, have been associated with COL I‐positive cells in circulation, which may also have occurred in dogs in this study 52 . Hence, further technical refinement with initial enrichment for circulating non‐leukocytes, as applied in human oncology, may be rewarding to develop 53 .…”

C-X-C-Chemokine-Receptor-Type-4 Inhibitor AMD3100 Attenuates Pulmonary Inflammation and Fibrosis in Silicotic Mice

“…The infiltrating fibrocytes may promote fibrosis progression and predict a poor prognosis in individuals with acute lung injury, acute respiratory distress syndrome, and IPF. 50 , 51 Li et al showed that fibrocytes co-expressing CD45 and collagen I were increased in the blood and lung of rats exposed to CS. 52 Here, we further confirmed that CFs co-expressing CD45, collagen I, and CXCR4 were increased in the lungs of mice after CS exposure, but that AMD3100 reduced the recruitment of CFs to lung tissue in mice.…”