Inhibitory Effect of Chaenomeles sinensis Fruit on Amyloid β Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice

Abstract

: The present study investigated an ethanol extract of Chaenomeles sinensis fruit (CSF) for possible neuroprotective effects on neurotoxicity induced by amyloid β protein (Aβ) (25-35) in cultured rat cortical neurons and also for antidementia activity in mice.

Inhibitory effect of an ethanol extract mixture of Aralia elata leaf, Chaenomeles sinensis fruit and Glycyrrhizae radix on Aβ (25–35)-induced memory impairment

“…Schwabach, Germany) to yield an ethanol extract (ACG, 55 g), which was then stored at room temperature until required. Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) was purchased from Bachem (Bubendorf, Switzerland). Acetylthiocholine iodide and 5,5'-dithiobis-2nitrobenzoic acid (DTNB) were purchased from Sigma Chemical (St. Louis, MO, USA).…”

“…Induction of memory impairment in mice and administration of ACG Memory impairment was induced by i.c.v. injection of 15 nmol of the aggregated form of Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35), as previously described [2]. ACG was administered orally 30 min before Aβ (25-35) injection on experimental day 1 (ED1) and then further administered daily for 7 days (to ED8).…”

“…In previous reports, Chaenomeles sinensis fruit was demonstrated to show neuroprotective effects against Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)-induced neurotoxicity in in vivo and in vitro [25]. Glycyrrhizae radix prevented Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)-induced neurotoxicity in cultured neurons [26]. Glycyrrhizae radix have been proved for its memory-enhancing effect and its anticholinesterase activity [23,27].…”

“…The present study was conducted to confirm the inhibitory effect of an ethanol extract mixture of Aralia elata, Chaenomeles sinensis fruit, and Glycyrrhizae radix, which was named as ACG, on memory impairment induced by a single intracerebroventricular (i.c.v.) injection of Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) in mouse model of AD-type amnesia [29].…”

Inhibitory Effect of an Ethanol Extract Mixture of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix on Amyloid β Protein (25-35)-Induced Neurotoxicity

“…There has been much effort to develop beneficial agents from medicinal plants with free radical scavenging and anti-inflammatory properties to achieve neuroprotection in our laboratory (Jung et al, 2012;Kim et al, 2011). In our previous studies, aerial parts, leaves and stems, of Vitis amurensis and Aralia cordata were demonstrated to show neuroprotective effect against Aβ (25-35)-induced neurotoxicity in in vivo and in vitro (Cho et al, 2009;Jeong et al, 2010).…”