“Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”

Martorano-Fernandes, Loyse; Cavalcanti, Yuri Wanderley; Almeida, Leopoldina de Fátima Dantas de

doi:10.1186/s12906-020-02893-9

Cited by 21 publications

(15 citation statements)

References 37 publications

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“…In general, the antimicrobial results displayed by EGP and ERP agree with data previously reported in the literature describing the potent antimicrobial activity of BRP and moderate to the weak antimicrobial potential of green propolis [23,27] . Moreover, these data agree with the literature emphasis that propolis extracts are more effective against Gram‐positive bacteria [8,9,11,28] .…”

Section: Resultssupporting

confidence: 90%

Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

Silva

Braun

et al. 2021

Chemistry & Biodiversity

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Brazilian green and red propolis stand out as commercial products for different medical applications. In this article, we report the antimicrobial activities of the hydroalcoholic extracts of green (EGP) and red (ERP) propolis, as well as guttiferone E plus xanthochymol (8) and oblongifolin B (9) from red propolis, against multidrug‐resistant bacteria (MDRB). We undertook the minimal inhibitory (MIC) and bactericidal (MBC) concentrations, inhibition of biofilm formation (MICB50), catalase, coagulase, DNase, lipase, and hemolysin assays, along with molecular docking simulations. ERP was more effective by displaying MIC and MBC values <100 μg mL−1. Compounds 8 and 9 displayed the lowest MIC values (0.98 to 31.25 μg mL−1) against all tested Gram‐positive MDRB. They also inhibited the biofilm formation of S. aureus (ATCC 43300 and clinical isolate) and S. epidermidis (ATCC 14990 and clinical isolate), with MICB50 values between 1.56 and 6.25 μg mL−1. The molecular docking results indicated that 8 and 9 might interact with the catalase's amino acids. Compounds 8 and 9 have great antimicrobial potential.

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Section: Resultssupporting

confidence: 90%

Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

Silva

Braun

et al. 2021

Chemistry & Biodiversity

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“…Propolis extracts also effectively eradicated biofilms formed by other dangerous bacteria, such as P. aeruginosa [ 30 , 31 , 32 ] and pathogenic fungi such as Candida spp. [ 33 , 34 , 35 ]. Our analysis of the literature has shown that another important problem that remains unresolved is the selection of appropriate methods for studying biofilm formation and assessing the effectiveness of antimicrobial agents in biofilm eradication.…”

Section: Discussionmentioning

confidence: 99%

Effect of Ethanol Extracts of Propolis (EEPs) against Staphylococcal Biofilm—Microscopic Studies

et al. 2020

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Staphylococci growing in the form of biofilm exhibit high resistance to a plethora of antibiotics. The aim of the study was to assess the influence of ethanolic extract of propolis (EEPs) on S. epidermidis ATCC 35984 biofilm using fluorescent microscopy. Propidium iodide (PI) and SYTO 9 were used for differentiation of live and dead cells, and calcofluor white was used to stain the extracellular matrix, the self-produced extracellular polymeric substances (EPS). The outcomes of the research confirm the promising potential of EEPs for eradication of staphylococcal biofilm. However, its activity cannot be classified as fully satisfactory, either in terms of the effectiveness of elimination of bacterial cells or disturbing the EPS structure. A two or even four times higher concentration of EEPs compared to MIC (Minimum Inhibitory Concentration) against planktonic cells (128 µg/mL) was necessary for effective (estimated for 90%) elimination of living cells from the biofilm structure. Unfortunately, even at that concentration of EEPs, the extracellular matrix was only partially disturbed and effectively protected the residual population of living cells of S. epidermidis ATCC 35984. In our opinion, a combination of EEPs with agents disrupting components of EPS, e.g., proteases, lysines, or enzymes degrading extracellular DNA or PIA (polysaccharide intercellular adhesin).

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“…Initially, titanium discs were immersed in 500 µL of artificial saliva composed of 1% carboxymethyl (w/v); 0.0084% sodium chloride (w/v); 0.12% potassium chloride (w/v); 0.0342% potassium phosphate (w/v); 0.0146% calcium chloride (w/v), and 0.052% magnesium chloride (w/v) [ 18 , 19 ]; following by incubation at 37 °C for 60 min [ 20 ]. Subsequently, the specimens were randomly and individually allocated into 24-well plates.…”

Section: Methodsmentioning

confidence: 99%

“…For co-culture biofilms, both C. albicans and S. salivarius were added to the inoculum. The plates were incubated at 37 °C under a micro-aerobic atmosphere using an anaerobic jar with a candle, which reduced the presence of oxygen, similar to a peri-implantitis-like microenvironment [ 20 ]. The culture medium was changed daily until the end of the experimental period (72 h).…”

Section: Methodsmentioning

confidence: 99%

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Interkingdom interaction between C. albicans and S. salivarius on titanium surfaces

Martorano-Fernandes¹,

Rodrigues²,

Borges³

et al. 2020

BMC Oral Health

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Background In oral candidiasis models, Candida albicans and Streptococcus salivarius sp. biofilms have an antagonistic relationship. Due to this, S. salivarius have been used experimentally as probiotic. However, the interaction between these microorganisms in the peri-implantitis-like microenvironment remains unknown. This study aimed to evaluate the interaction between C. albicans and S. salivarius biofilms developed on titanium surfaces, under reduced oxygen levels. Methods Titanium specimens were pre-conditioned with artificial saliva (1 h, 37 °C). Single-species biofilms of C. albicans (ATCC 90028) and co-culture biofilms of C. albicans and S. salivarius (ATCC 7073) was developed for 24 and 72 h on titanium specimens. Subsequently, the effect of these intervals of biofilm formation and the interactions among the cells were evaluated. Biofilms from cultures were collected and analyzed for cell viability (CFU/mL), biofilm biomass, and total protein dosage. Data were analyzed using Mann–Whitney test (α = 5%). In addition, co-culture biofilms were analyzed using fluorescence microscopy. Results C. albicans growth did not change due to the presence of S. salivarius. Besides, co-culture biofilms showed a significant difference in the number of viable cells between 24 and 72 h of biofilm development (p < 0.05). The highest biofilm biomass and protein dosage were observed in co-cultures at 72 h of biofilm development. Fluorescence microscopy showed that co-cultures biofilms at 24 h have limited number of pseudo-hyphal and hyphae cells of C. albicans. At 72 h, these types of cells have increased. S. salivarius in both stages of development was present in some clusters surrounded by C. albicans. Conclusions Co-cultivation of C. albicans with S. salivarius in biofilms developed on titanium surfaces, under lower oxygen levels, did not affect fungus growth. In addition, S. salivarius did not hind C. albicans virulence. These findings suggest that the use of S. salivarius as a probiotic would be ineffective in peri-implant disease treatment.

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“Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”

Cited by 21 publications

References 37 publications

Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

Effect of Ethanol Extracts of Propolis (EEPs) against Staphylococcal Biofilm—Microscopic Studies

Interkingdom interaction between C. albicans and S. salivarius on titanium surfaces

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