Inhibitory effect of Astragalus Membranaceus on osteoporosis in SAMP6 mice by regulating vitaminD/FGF23/Klotho signaling pathway

Abstract

Spontaneous senile osteoporosis severely threatens the health of the senior population which has emerged as a severe issue for society. A SAMP6 mouse model was utilized to estimate the impact of intragastrically administered Astragalus Membranaceus (AR) on spontaneous senile osteoporosis. Bone mineral density (BMD) and bone microstructure were measured using Micro-CT; contents of calcium and phosphorus were determined with the colorimetric method; and gene and protein expressions of fibroblast growth factor 23 (FGF23), Klotho, Vitamin D receptor (VDR), CYP27B1 and CYP24A1 were detected using qPCR, Western blot and ELISA assays, respectively. The findings indicated that AR could improve the femoral BMD and bone microstructure, elevate the contents of calcium and phosphorus, and increase the expression of Klotho, VDR, and CYP27B1 whereas decreasing the expression of FGF23 and CYP24A1 in SAMP6 mice in a dose independent manner. The present study has demonstrated that AR can promote osteogenesis and alleviate osteoporosis. It is also expected to provide a new insight for the treatment of spontaneous senile osteoporosis and to serve as a research basis for AR application.

Application of Huangqi Guizhi Wuwu Decoction in Orthopaedics and Traumatology

“…5 6 7 Researches have found that certain components in Huangqi (Radix Astragali) can promote the formation of osteoblasts, delay osteoporosis, and may be beneficial for senile osteoporosis. 8 It has also confirmed that astragaloside IV in Huangqi (Radix Astragali) can promote the differentiation of bone marrow–derived mesenchymal stem cells which may provide new ideas for the treatment of osteoporosis. 9 At present, studies have also verified that Huangqi (Radix Astragali) has the effects of antioxidant, anti-inflammatory, analgesic, anticancer, thrombosis preventing, hypoglycemic, lipid regulating, immune regulating, and antiaging.…”

Preparation and characterization of scutellarin loaded on ultradeformable nano-liposomes scutellarin EDTMP (S-UNL-E) and in vitro study of its osteogenesis

“…Substrates I and II were added to the sample and mixed well, and a termination solution was added and mixed well. The sample was detected by enzyme labeling at 450 nm wavelength [ 24 ].…”

Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy

“…Bge. 14 The main components of AM are saponins, polysaccharides, and flavonoids, which have a variety of biological functions such as immune regulation, antitumor, antivirus, antiaging, and hypoglycemic. 15 In the treatment of SLE, AM could increase the curative effect, reduce the side effects of glucocorticoid and reduce the infection through multiple ways of immunomodulation.…”

“…Hsiao and AM (Fisch.) Bge 14 . The main components of AM are saponins, polysaccharides, and flavonoids, which have a variety of biological functions such as immune regulation, antitumor, antivirus, antiaging, and hypoglycemic 15 .…”