Inhibitory effect of Amomum villosum water extracts on α-glucosidase activity

Kim, Ha-Rim; Antonisamy, Paulrayer; Kim, Yeseul; Lee, Guemsan; Ham, Hyang-Do; Kwon, Kang‐Beom

doi:10.1016/j.pmpp.2021.101779

Cited by 9 publications

(4 citation statements)

References 27 publications

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“…Presently, oral synthetic diabetic agents such as acarbose, voglibose, and miglitol are used to treat type-2 DM that regrettably cause side effects, including flatulence, diarrhea, and abdominal disorder (Wang et al 2022). Inhibition of α-glucosidase is one of the approaches to treating T2DM (Kim et al 2022). Accordingly, recent updates on lower blood glucose level treatment showed a trend of consuming medicinal plants due to their ability.…”

Section: Introductionmentioning

confidence: 99%

The potency of selected ethnomedicinal plants from East Kalimantan, Indonesia as antidiabetic agents and free-radical scavengers

et al. 2022

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Abstract. Ramadhan R, Phuwapraisirisan P, Amirta R, Darmawan MFB, Ul-Haq K, Kusuma IW, Suwito H, Abdulgani N, Mukhdlor A, Saparwadi. 2022. The potency of selected ethnomedicinal plants from East Kalimantan, Indonesia as antidiabetic agents and free-radical scavengers. Biodiversitas 23: 2225-2231. Ten ethnomedicinal plant extracts from East Kalimantan flora, traditionally used to treat blood sugar levels and other diabetes-related diseases, were examined in vitro for their antidiabetic and free radical scavenging activities by inhibiting rat alpha-glucosidase and several free radicals such as DPPH, ABTS, and Nitric oxide respectively. Out of the ten plant species investigated for their antidiabetic activity against maltase and sucrase rat alpha-glucosidase, three exhibited the strongest ?-glucosidaseinhibitory activity with maltose as a substrate, namely extracts of Garcinia nervosa, Syzygium caudatilimbum, and Shorea balangeran with IC50 values of 0.046; 0.037; 0.045 mg/mL. Meanwhile, quercetin as a positive control appeared to have a comparable IC50value. Furthermore, among the ten extracts, Syzygium caudatilimbum, Shorea balangeran, and Ixora javanica showed good inhibition against sucrase rat alpha-glucosidase. Moreover, the antioxidant test showed that the ten methanol extracts of plants from East Kalimantan have an antioxidant activity indicated by IC50values. The present study confirms that the ethnopharmacological use of selected plants from East Kalimantan might have potential as an antidiabetic and natural antioxidant.

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Section: Introductionmentioning

confidence: 99%

The potency of selected ethnomedicinal plants from East Kalimantan, Indonesia as antidiabetic agents and free-radical scavengers

et al. 2022

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“…A. villosum water extract was investigated for its α-glucosidase activity at different concentrations of 1, 3 and 5 mg/mL, which proportionally increased the inhibition against rat α-glucosidase with IC50 of 31.99 ± 6.79%, 48.85 ± 4.75% and 62.58 ± 6.69%, respectively. Although A. villosum water extract has lower inhibition on α-glucosidase than the reference acarbose, it showed a considerable drop in blood glucose levels in the sucrose loading test when administered to the rats compared to the control group [ 59 ].…”

Section: Plant Extracts As α-Glucosidase Inhibitor Sourcesmentioning

confidence: 99%

Recent Updates on Phytoconstituent Alpha-Glucosidase Inhibitors: An Approach towards the Treatment of Type Two Diabetes

Kashtoh

Baek

2022

Plants

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Diabetes is a common metabolic disorder marked by unusually high plasma glucose levels, which can lead to serious consequences such as retinopathy, diabetic neuropathy and cardiovascular disease. One of the most efficient ways to reduce postprandial hyperglycemia (PPHG) in diabetes mellitus, especially insulin-independent diabetes mellitus, is to lower the amount of glucose that is absorbed by inhibiting carbohydrate hydrolyzing enzymes in the digestive system, such as α-glucosidase and α-amylase. α-Glucosidase is a crucial enzyme that catalyzes the final stage of carbohydrate digestion. As a result, α-glucosidase inhibitors can slow D-glucose release from complex carbohydrates and delay glucose absorption, resulting in lower postprandial plasma glucose levels and control of PPHG. Many attempts have been made in recent years to uncover efficient α-glucosidase inhibitors from natural sources to build a physiologic functional diet or lead compound for diabetes treatment. Many phytoconstituent α-glucosidase inhibitors have been identified from plants, including alkaloids, flavonoids, anthocyanins, terpenoids, phenolic compounds, glycosides and others. The current review focuses on the most recent updates on different traditional/medicinal plant extracts and isolated compounds’ biological activity that can help in the development of potent therapeutic medications with greater efficacy and safety for the treatment of type 2 diabetes or to avoid PPHG. For this purpose, we provide a summary of the latest scientific literature findings on plant extracts as well as plant-derived bioactive compounds as potential α-glucosidase inhibitors with hypoglycemic effects. Moreover, the review elucidates structural insights of the key drug target, α-glucosidase enzymes, and its interaction with different inhibitors.

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“…The p -nitrophenol glucopyranoside ( p NPG) colorimetry is the most widely employed and traditional method for screening α-Glu inhibitors. To date, the p NPG colorimetric method has been extensively employed for the screening of natural compounds derived from a wide range of traditional Chinese medicine extracts, in terms of their inhibitory activity against α-Glu. , However, this method is susceptible to interference from sample absorption in the vicinity of the measuring wavelength at 405 nm . Therefore, it is crucial to develop a more sensitive and universally applicable strategy for screening α-Glu inhibitors.…”

Section: Introductionmentioning

confidence: 99%

Cerium-Based Metal–Organic Framework Nanocrystals for Colorimetric Screening of α-Glucosidase Inhibitors

Wan,

Chen

2024

ACS Appl. Nano Mater.

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The screening method for α-glucosidase (α-Glu) inhibitors plays a crucial role in the development of antidiabetic drugs. This study introduces cerium-based metal–organic framework (Ce-MOF) nanocrystals for the highly sensitive colorimetric detection of α-Glu inhibitors. The synthesized Ce-MOF nanocrystals exhibited excellent oxidase-like activity, efficiently catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a blue product (oxTMB) with a strong absorption peak at 652 nm, enabling quantitative analysis using spectroscopic techniques. Notably, hydroquinone (HQ) can reduce the blue oxTMB to the colorless TMB. Specifically, α-Glu can hydrolyze α-arbutin to generate HQ, which subsequently reduces the oxidase activity of Ce-MOF. However, the addition of α-Glu inhibitors in the system limited the production of HQ, thereby maintaining the oxidase-like activity stability of Ce-MOF. Leveraging this mechanism, a screening method for α-Glu inhibitors was developed. This method overcomes the limitations of the conventional p-nitrophenyl-α-d-glucopyranoside (p-NPG) assay and effectively avoids potential interference from hydrogen peroxide introduced by peroxidase-like nanomaterials, significantly enhancing the detection sensitivity. The reliability and accuracy of this method were validated, demonstrating the highly sensitive determination of acarbose with an IC50 value of 7.04 μM and a detection limit of 3.2 nM. Moreover, this method has been successfully applied to screen potential α-Glu inhibitors from natural flavonoid compounds.

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Inhibitory effect of Amomum villosum water extracts on α-glucosidase activity

Cited by 9 publications

References 27 publications

The potency of selected ethnomedicinal plants from East Kalimantan, Indonesia as antidiabetic agents and free-radical scavengers

The potency of selected ethnomedicinal plants from East Kalimantan, Indonesia as antidiabetic agents and free-radical scavengers

Recent Updates on Phytoconstituent Alpha-Glucosidase Inhibitors: An Approach towards the Treatment of Type Two Diabetes

Cerium-Based Metal–Organic Framework Nanocrystals for Colorimetric Screening of α-Glucosidase Inhibitors

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