MATERIALS AND METHODS Experiments were conducted to study the interaction of ethylene and the cytokinin N6-benzyladenine (BA) partially closed cotyledons. When ethylene production was measured over time, absorbent wadding discs with 10 seedlings each were transferred from Petri dishes to 250-ml wide-mouth Erlenmeyer flasks, 2.5 to 3 h after treatment. Flasks were stoppered and returned to the growth chamber. Ethylene Measurement. At appropriate times after treatment, 2-ml gas samples from the flasks were analyzed for ethylene. All analyses were performed by GLC with a Perkin-Elmer (Norwalk, CT) model 990 gas chromatograph equipped with 122-x 3.2-mm stainless steel columns of 80/100 mesh Poropak N (Supelco, Inc., Bellefonte, PA) and flame ionization detectors. The carrier was prepurified nitrogen at a flow rate of 40 ml/min. The flame was a hydrogen/air flame provided by zero grade air and ultra-high purity hydrogen (Linde, South Plainfield, NJ). Operating temperatures for the injection block, column oven, and manifold were 105, 70, and 105 C, respectively. The sensitivity of this system allowed consistent detection of 20 pl of ethylene in a 2-ml sample. Retention time for ethylene was 44 ± 2 s. Ethylene concentrations were calculated from peak area determinations of known ethylene concentrations by using a 1.0 ,ll/l ethylene in nitrogen standard prepared by Matheson Gas Products (East Rutherford, NJ).Ethylene Flow Experiments. To examine the response of seedlings to applied ethylene, seedlings were exposed to different concentrations of ethylene in a continuous flow system for periods of 48 to 70 h. At the same time, control seedings were exposed to ethylene-free air in a parallel system. Each gas line contained six 250-ml flasks arranged in series. Ethylene concentrations were varied between 10 ± 5 nl/l and 110 ± 20 nl/l (depending upon the experiment) at a flow rate of 0.5 I/min. The air line provided ethylene-free air at the same rate. Gas from inlet and outlet of air and ethylene lines was periodically sampled and analyzed for ethylene throughout the treatment period. Desired ethylene concentrations were produced by continuous flow mixing of appro-