Inhibitory combinations of nisin, sodium chloride, and pH on Listeria monocytogenes ATCC 15313 in broth by an experimental design approach

Bouttefroy, A.; Mansour, Marianne; Linder, Michel; Millière, Jean-Bernard

doi:10.1016/s0168-1605(99)00171-3

Cited by 45 publications

(35 citation statements)

References 16 publications

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“…The salt inhibition was also reported in other known CS␣␤-type antimicrobial peptides such as insect defensins, plant defensins, and drosomycin (see introduction). The antimicrobial activities of cationic lantibiotics (e.g., nisin) are also inhibited by salts (2). The inhibition by salts seems to be a hallmark of antimicrobial peptides as a class.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial Properties of Recombinant ASABF, an Antimicrobial Peptide Isolated from the Nematode Ascaris suum

Zhang

Yoshida

Aizawa

et al. 2000

Antimicrob Agents Chemother

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ASABF is a CS␣␤-type antimicrobial peptide that contains four intramolecular disulfide bridges (Y. Kato and S. Komatsu, J. Biol. Chem. 271: [30493][30494][30495][30496][30497][30498] 1996). In the present study, a recombinant ASABF was produced by using a yeast expression system, and its antimicrobial activity was characterized in detail. The recombinant ASABF was active against all gram-positive bacteria tested (7 of 7; minimum bactericidal concentration [MBC], 0.03 to 1 g/ml) except Leuconostoc mesenteroides, some gram-negative bacteria (8 of 14; MBC, >0.5 g/ml), and some yeasts (3 of 9; MBC >3 g/ml). Slight hemolytic activity (4.2% at 100 g/ml) against human erythrocytes was observed only under low-ionic-strength conditions. Less than 1 min of contact was enough to kill Staphylococcus aureus ATCC 6538P. The bactericidal activity against S. aureus was inhibited by salts.CS␣␤-type antimicrobial peptides contain a single ␣ helix and a pair of antiparallel ␤ sheets stabilized by three or four intramolecular disulfide bridges (9). Insect defensins that contain three intramolecular disulfide bridges (7, 12), drosomycin (isolated from the fruit fly [Drosophila melanogaster]) (16), and plant defensin (3), which contains four intramolecular disulfide bridges, have been experimentally demonstrated to be members of a CS␣␤-type antimicrobial peptide by three-dimensional structural analyses. Some antimicrobial peptides that contain the consensus sequence of insect defensins (6) have been isolated from other arthropods and mollusks (4, 6, 10, 13), suggesting that they should also be CS␣␤-type antimicrobial peptides. Although these peptides form similar three-dimensional structures, the CS␣␤-type antimicrobial peptides exhibit diversified antimicrobial spectra. For instance, insect defensins are active against gram-positive bacteria but not against gram-negative bacteria or eukaryotic microbes (9), despite some exceptions (16). In contrast, drosomycin is active against fungi but not against bacteria (14). Plant defensins are categorized into four groups with different antimicrobial spectra, i.e., group I (active against gram-positive bacteria and fungi), group II (active against fungi but inactive against bacteria), group III (active against gram-positive and gram-negative bacteria but inactive against fungi), and group IV (active against gram-positive and gram-negative bacteria and fungi) (11). In addition, the antimicrobial activities of some CS␣␤-type antimicrobial peptides are inhibited by salts (5, 17), but those of others are not (15). However, the structure-activity relationship that can explain such diversified antimicrobial characteristics has not been well elucidated. Characterization of novel CS␣␤-type antimicrobial peptides has been providing novel antimicrobial substances with unique properties (15).ASABF is a novel CS␣␤-type antimicrobial peptide that contains four intramolecular disulfide bridges isolated from the nematode Ascaris suum (14). In this study, a recombinant ASABF was produced with a yeast express...

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Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial Properties of Recombinant ASABF, an Antimicrobial Peptide Isolated from the Nematode Ascaris suum

Zhang

Yoshida

Aizawa

et al. 2000

Antimicrob Agents Chemother

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“…6A). The bactericidal effect of nisin against Listeria monocytogenes is the highest at low (near 0%) or high NaCl concentration (near 6%), while between 2 and 4% NaCl, the activity of nisin is low (Bouttefroy et al, 2000). The bactericidal activity of heat-treated Lz at 80 ˚C for 20 min at pH 6.0, termed HL80/6, against both E. coli and S. aureus is abolished by 0.1% and 1% NaCl (Ibrahim et al, 1996).…”

Section: Resultsmentioning

confidence: 98%

Antimicrobial Activity of Duck Egg Lysozyme Against Salmonella enteritidis

Naknukool¹,

Hayakawa²,

Uno³

et al. 2009

Global Issues in Food Science and Technology

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The objective of the study was to investigate antimicrobial activity of native and reduced forms of duck lysozyme (dLz) against Salmonella enteritidis. Moreover, antimicrobial activity of dLz was compared with chicken lysozyme (cLz). The purified dLz was reduced with dithiothreitol. Free SH groups of reduced dLz were trapped with Combination of lactoferrin (0.1 mg/ml) and reduced dLz (0.1 mg/ml) showed a synergistic effect. In all conditions, dLz showed higher activity than cLz in both native and reduced forms. The result of the study indicated that reduced dLz trends to be a more efficient antimicrobial agent against S. enteritidis. To achieve the highest sufficiency, the essential roles of food components and incubation temperature should be concerned in reduced dLz application.

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“…The results of the present study about susceptibility of the bacterium to reduction of pH and increasing of incubation temperature and NaCl concentration are agreement with other studies. 5,25,[34][35][36] Based on our results, the pHs 5 and 6 significantly decreased the number of L. monocytogenes in all concentrations. Boziaris et al reported the most reduction growth of L. monocytogenes was occurred at pH 4.81.…”

Section: Antimicrobial Effect Of Essential Oil and Nisin In Differentmentioning

confidence: 93%

“…34 As well as, Bouttefroy et al and Rohani et al found that 2.5g/100 ml and 4.5g/100 ml NaCl concentrations and increasing incubation temperatures (20°C and 30°C) were enhanced the anti-listerial activities of nisin and various essential oils. 5,35 In the present study, essential oil and nisin alone at concentrations shown to be non-inhibitory (two concentrations lower than the MIC), were combined with each other (four combination) as described in Tables 2, 3 and 4. Previous study of authors indicated that M. spicata essential oil or nisin alone had a slight effect of the growth of L .monocytogenes in the laboratory medium.…”

Section: Antimicrobial Effect Of Essential Oil and Nisin In Differentmentioning

confidence: 99%

The Combined Effect of Mentha spicata Essential Oil and Nisin Against Listeria monocytogenes

Moosavy¹,

Shahbazi²,

Shavisi³

2015

Pharm Sci

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Inhibitory combinations of nisin, sodium chloride, and pH on Listeria monocytogenes ATCC 15313 in broth by an experimental design approach

Cited by 45 publications

References 16 publications

In Vitro Antimicrobial Properties of Recombinant ASABF, an Antimicrobial Peptide Isolated from the Nematode Ascaris suum

In Vitro Antimicrobial Properties of Recombinant ASABF, an Antimicrobial Peptide Isolated from the Nematode Ascaris suum

Antimicrobial Activity of Duck Egg Lysozyme Against Salmonella enteritidis

The Combined Effect of Mentha spicata Essential Oil and Nisin Against Listeria monocytogenes

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