Antimicrobial Activity of Duck Egg Lysozyme Against Salmonella enteritidis

Naknukool, Supaporn; Hayakawa, Shigeru; Uno, Takahiro; Ogawa, Masahiro

doi:10.1016/b978-0-12-374124-0.00018-1

Cited by 8 publications

(15 citation statements)

References 33 publications

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“…Protein ovotransferrin dari dimurnikan menggunakan modifikasi metode dari penelitian Naknukool et al (2009). Hal yang dimodifikasi adalah penambahan 0,01 mM HCl untuk menurunkan pH membrane dan penggunaan larutan pencuci resin.…”

Section: Pemurnian Ovotransferrin Dari Membran Kerabangunclassified

“…Beberapa peneliti enzim, pada umumnya mendapatkan hasil absorbansi fraksi yang kurang dari 1,00 (Naknukool et al, 2009;Al-Baarri et al, 2010). Hasil absorbansi yang menunjukkan angka kurang dari 1,00 tergolong angka yang sensitif dan dapat mengetahui pola perubahan absorbansi masingmasing fraksi.…”

Section: Kadar Protein Kualitatifunclassified

“…Purifikasi protein yang dilakukan oleh Naknukool et al (2009), menggunakan larutan pendahuluan berupa acetate buffer molaritas rendah (10 mM), yang berguna untuk memisahkan senyawa pengganggu termasuk sisa lemak dan mineral. Namun dalam penelitian ini, tidak digunakan buffer tersebut karena hasil elusi fraksi pertama dinilai sudah jernih dan tidak keruh.…”

Section: Kadar Protein Kualitatifunclassified

“…Puncak nilai absorbansi, fraksi yang tertinggi pada penelitian Naknukool et al (2009), ditunjukkan pada fraksi yang dielusikan dengan 0,5 mM NaCl dengan nilai absorbansi sekitar 2,50 sedangkan pada penelitian ini, absorbansi yang tertinggi dicapai pada fraksi 1 yang merupakan hasil elusi dari 0,1 mM NaCl. Nilai absorbansi sangat bergantung pada zat yang dipurifikasi.…”

Section: Kadar Protein Kualitatifunclassified

“…Semakin tinggi kandungan protein, maka semakin tinggi nilai puncak absorbansi. Naknukool et al (2009) menggunakan putih telur yang mempunyai kandungan protein sebesar 88,63% (Ayadi et al, 2008) sedangkan pada penelitian ini, digunakan membran telur yang hanya mempunyai kandungan protein berkisar 3,5-4,0%, sehingga terjadi perbedaan yang cukup mencolok pada nilai absorbansi titik tertingginya.…”

Section: Kadar Protein Kualitatifunclassified

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Purifikasi Dan Profil Protein Ovotransferrin Dari Eggshell Membrane Telur Ayam Ras Dan Buras

Siregar

Al-Baarri

Hintono

et al. 2016

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Eggs consumption in Indonesia increases every year resulting in egg shell waste. In addition, the functional components including ovotransferrin existing in this waste has not b een yet studied well. This research has b een done to explore ovotransferrin in egg shell membrane from local Indonesia and leghorn hens. The ion exchange chromatography using SP-Sepharose Fast Flow was used to purify the ovotransferrin from egg shell memb rane. The fractions from salt elution were analyzed for protein concentration and protein profile using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result showed that the b est concentration of salt elution to ob tain ovotransferrin was 0.1 mM NaCl since the single b and was detected among other fractions. The ovotransferrin concentration (%) per single egg was also calculated as much as 0.75 ±0.017 and 0.16±0.005 for leghorn dan local Indonesia hen, respectively. This result may provide the b eneficial information to purify ovotransferrin from egg shell memb rane which conceivab ly as a food preservative agent.

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Section: Pemurnian Ovotransferrin Dari Membran Kerabangunclassified

Section: Kadar Protein Kualitatifunclassified

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Purifikasi Dan Profil Protein Ovotransferrin Dari Eggshell Membrane Telur Ayam Ras Dan Buras

Siregar

Al-Baarri

Hintono

et al. 2016

jtip

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Encapsulating an Immunosuppressant Enhances Tolerance Induction by Siglec‐Engaging Tolerogenic Liposomes

et al. 2017

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Unwanted antibody responses significantly impact human health, and current options for treating deleterious antibody responses largely rely on broad immunosuppressants that can compromise overall immunity. A desirable alternative is to induce antigen-specific immune tolerance. We have shown that co-presentation of antigen and ligands of B cell Siglecs (sialic acid-binding immunoglobulin-like lectin) on a liposomal nanoparticle induces antigen-specific tolerance. Although Siglec-engaging tolerance-inducing antigenic liposomes (STALs) induce robust B cell tolerance in naïve mice, the full potential of STALs requires long-term tolerance induction and suppression of an ongoing immune response. We hypothesized that STALs encapsulated with rapamycin (RAPA), an immunomodulator, could improve the efficacy of STALs and potentially enable their use in the context of immunological memory. Here, we show that formulation of STALs with RAPA produces enhanced tolerance induction in naïve mice compared to STALs without RAPA, but has minimal impact on inducing tolerance in previously sensitized mice. These findings indicate that the addition of immunomodulator to STALs could be beneficial in tolerance induction and support future development of STALs for the treatment of allergy and autoimmune diseases.

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A Two-Step, One-Pot Enzymatic Method for Preparation of Duck Egg White Protein Hydrolysates with High Antioxidant Activity

Ren

et al. 2013

Appl Biochem Biotechnol

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Biocatalytic hydrolysis reactions were designed for preparation of bioactive hydrolysate of duck egg white protein (DEWP) employing two enzymes in one pot. Firstly, the fresh DEWP was thermal treated at 95 °C, for 40 min at pH 10, to effectively deactivate enzyme inhibitors thus facilitating the following two-step enzymatic hydrolysis. Compared with single-enzyme processes, the two-step enzymatic procedures showed much higher reaction efficiency. The first enzymatic step (in the presence of Alcalase or hydrolase SEEP) allowed to hydrolyze DEWP with degree of hydrolysis (DH) of 8.8-10% and soluble peptide yield (SEP) of 60.5-70.2% in a short period (4 h). The second enzymatic step (in the presence of Trypsin or Alcalase) gave a further degradation of DEWP with DH and SEP being more than 26.2% and 90.4%, respectively. The final hydrolysates exhibited high antioxidant activity in an evident DH dependent manner. The hydrolysates achieved by sequential addition of the proteinase SEEP and Alcalase at DH value 21% gave the highest antioxidant activity, which was mainly ascribed to the changes in the amino acid compositions that the contents of some key amino acids and total hydrophobic amino acids were significantly improved by the enzymatic hydrolysis.

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Antimicrobial Activity of Duck Egg Lysozyme Against Salmonella enteritidis

Cited by 8 publications

References 33 publications

Purifikasi Dan Profil Protein Ovotransferrin Dari Eggshell Membrane Telur Ayam Ras Dan Buras

Purifikasi Dan Profil Protein Ovotransferrin Dari Eggshell Membrane Telur Ayam Ras Dan Buras

Encapsulating an Immunosuppressant Enhances Tolerance Induction by Siglec‐Engaging Tolerogenic Liposomes

A Two-Step, One-Pot Enzymatic Method for Preparation of Duck Egg White Protein Hydrolysates with High Antioxidant Activity

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