Inhibitory activity of chlorogenic acid on enzymes involved in the fatty acid synthesis in animals and bacteria

Abstract

SummaryIt was found that chlorogenic acid inhibited in vitro animal fatty acid synthase (FAS I) and the b-ketoacyl-ACP reductase (FabG) from Escherichia coli in a concentration-dependent manner with respective IC50 of 94.8 and 88.1 mM. The results of LineweaverBurk plots indicated that chlorogenic acid inhibited competitively the binding of NADPH to FAS I, while left those of acetyl-CoA and malonyl-CoA unaffected. Further kinetic studies showed that chlorogenic acid blocked the activity of FAS I mainly by inhibiting the b-ketoacyl reductase domain, which catalyzed the same reaction as that done by FabG in the fatty acid synthesis. The b-ketoacyl reduction reactions accomplished by both FAS I and FabG required nucleotide cofactor, NADPH. Furthermore, the Lineweaver-Burk and Yonetani-Theorell analyses implicated that chlorogenic acid filled competitively in the binding-pocket of NADPH in the b-ketoacyl reductase domain of FAS I. The similar results were also obtained from the inhibition of FabG by chlorogenic acid. As observed in these results, the inhibitions of FAS I and FabG by chlorogenic acid were highly related to the interference of the inhibitor with NADPH, which was possibly due to the similarity between chlorogenic acid and some portion of NADPH, maybe the section consisting of the two ribose groups.

Effect of Peucedanum japonicum Thunb on the Expression of Obesity-Related Genes in Mice on a High-Fat Diet

“…Our preliminary results showed that PJT was rich in polyphenol, which inhibits lipase to reduce lipid absorption, as suggested for tea catechins 20 . Furthermore, several lines of in vitro studies have demonstrated the occurrence of hypolipidemic compounds in PJT leaves 9,10 . It is, therefore, likely that these active components may infl uence the lipid dynamics, in concert, to prevent the mice from developing obesity.…”

Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb

“…Antibacterial activities of hydro-distilled essential oils of whole parts of P. japonicum indicating anti-inflammatory and potential health benefits on human skin have been reported (Yang et al 2009). Furthermore, several in vitro studies have demonstrated the occurrence of hypolipidaemic compounds in leaves of P. japonicum (Hsu and Yen 2007; Li et al 2006). While studying the radical scavenging activity of P. japonicum , (Hisamoto et al 2003) isolated 17 compounds from the leaves, of which several compounds played an important role in the potent antioxidant activity.…”

Anti-Obesity and Hypotriglyceridemic Properties of Coffee Bean Extract in SD Rats

“…The reduction in the serum and hepatic triglyceride levels in CBE-fed rats is thought to be induced by both the suppression of fatty acid synthesis in hepatic cytosol and the acceleration of fatty acid oxidation in hepatic mitochondria. Chlorogenic acid has been shown to inhibit FAS activity (Li et al, 2006), while there are few reports that caffeine affects the activities of fatty acid synthetic enzymes. Therefore, chlorogenic acid in CBE may be responsible for the suppression of fatty acid synthesis in the liver.…”