Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb

Abstract

Background Peucedanum japonicum Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed to develop an in vitro plant regeneration system of P. japonicum via somatic embryogenesis and to analyse chlorogenic acid and rutin contents in a few commercially available plant products of P. japonicum in Japan and Taiwan markets, and tissue culture plants derived from somatic embryos.ResultsInduction of somatic embryogenesis could be achieved when root derived calli after three subcultures were transferred from Murashige Skoog’s salts and vitamins (MS basal) medium with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.1–5 mg/L) to a medium with abscisic acid (ABA) (0.5–4 mg/L), or exposed to eight different light spectra provided by light-emitting diode (LED) sources. Concentrations of ABA and LED light spectra had an influence on number of somatic embryos induced and proliferation of callus. Development of secondary somatic embryos and conversion of embryos to plantlets was achieved on a medium with ABA, or their exposure to red or blue lights in a special incubation chamber. Four months old tissue culture plants derived from somatic embryos showed significantly higher levels of chlorogenic acid (10.5 mg/g dw) compared to commercial product sold in Japanese market (0.55 mg/g dw). However, rutin was absent in tissue culture plants in contrast to commercial sample (0.33 mg/g dw).ConclusionIn this report, we describe in vitro plant regeneration system in P. japonicum via somatic embryogenesis and production of chlorogenic acid in tissue culture plants. The present study has application in further tissue culture propagation of elite plant material with high chlorogenic acid content, and identification of high yielding plants with the LC–MS method.

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

“…The response of plant cells to stress and their reorientation to developmental programs results in the expression of protein kinases, transcription factors, and structural genes that contribute to the adaptation [ 45 ]. Similar to the present study, the beneficial effects of LED lights on the contents of bioactive compounds in Peucedanum japonicum Thunb have been reported in our laboratory [ 22 ].…”

“…LED lids CW-5000K and WW-2700K represented cool (C) and warm (W) white (W) light, while 5000K and 2700K represented color temperature, respectively. As reported in a previous study from our laboratory [ 22 ], six of these LED lids emitted single or combinations of four different light spectra with wavelengths such as blue (450 nm), green (525 nm), red (660 nm), and far-red (730 nm). The symbols in each LED lid code and the spectral distribution (quantum ratio) in eight LED lids were as follows: blue (B), green (G), red (R), infrared (IR), CW-5000K (28:43:29:0), WW-2700K (8:46:46:0), 8R1B (16:0:84:0), 7R1G1B (17:9:74:0), 3R3B3IR (57:0:43:37), 9R (0:0:100:0), 9B (100:0:0:0), and 9IR (0:0:0:100).…”

“…Recently, Pedmale and co-workers reported that the quality of light affects plant growth and development by the regulation of different mechanisms, including the selective activation of light receptors, such as phytochromes by red and far-red light, cryptochromes and phototropins by blue light, and UV-B receptors by ultraviolet light [ 35 ]. In a previous study in our laboratory, LED lights affected the development of somatic embryos and callus proliferation (fresh weight) in Peucedanum japonicum Thunb [ 22 ]. Several studies have demonstrated that the quality, duration, and intensity of red, infrared, blue, and ultraviolet light can have a profound influence on plants by activating or deactivating physiological reactions and controlling their growth and development [ 36 , 37 , 38 ].…”

LED illumination and plant growth regulators’ effects on growth and phenolic acids accumulation in Moluccella laevis L. in vitro cultures

“…The highest contents of chlorogenic acid (15.5-18.32 mg/100 g DW) and caffeic acid (12.10-14.12 mg/100 g DW) were in extracts from shoots cultured under both illumination conditions (Table 5). In contrast, Chen et al (2016) boosted content of chlorogenic acid in Peucedanum japonicum calli by adding infrared wavelengths to the light spectrum. Conversely, in another Lamiaceae plant, Scutellaria lateriflora, the blue light induced significant increase in accumulation of phenolic metabolites (Kawka et al 2017).…”

Protective Effects of Peucedanum japonicum Extract against Osteoarthritis in an Animal Model Using a Combined Systems Approach for Compound-Target Prediction

“…The above criteria were used to select the final set of compounds ( n = 12; Table S1 ) as candidates for subsequent analysis. These compounds are the main components/active functional ingredients of PJ [ 10 , 11 , 13 , 14 , 27 ].…”