Inhibitory Activities of Surface Associated Bacteria Isolated from the Marine Sponge Pseudoceratina purpurea

Kanagasabhapathy, Manmadhan; Sasaki, Hideaki; Nakajima, Kazuhiko; Nagata, Kumiko; Nagata, Shinichi

doi:10.1264/jsme2.20.178

Cited by 13 publications

(9 citation statements)

References 31 publications

(30 reference statements)

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“…from seagrass Enhalus acoroides inhibited the growth of biofilm-forming bacteria. A study by Kanagasabhapathyt et al (2005) demonstrated the inhibition of growth of fouling bacteria by sponge Pseudoceratina purpure-associated bacterium Virgibacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Characterization of carotenoid pigments from bacterial symbionts of soft-coral Sarcophyton sp. from North Java Sea

Kusmita¹,

Mutiara²,

Nuryadi

et al. 2017

Int Aquat Res

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Marine bacteria produce natural pigments; however, the ability of marine bacterial symbionts to produce natural pigments has been less studied. Marine bacteria associated with soft-coral Sarcophyton sp. collected from Karimunjawa Island were successfully isolated and screened to synthesize the carotenoid pigments. This approach has allowed the use of these symbionts as an environmental friendly source of new natural pigments. Out of 33 bacterial isolates, only 4 bacterial symbionts (CBSCP 2-2, CBSCP 2-3, CBSCP 1-1, and CBSCP 2-4), positively contain carotenoid pigments. Molecular identification based on 16S rDNA method showed that bacterial symbionts CBSCP 2-2, CBSCP 2-3, CBSCP 1-1, and CBSCP 2-4 were closely related to Pseudoalteromonas shioyasakiensis, Pseudoalteromonas rubra, Virgibacillus salaries, and Pseudoalteromonas spongiae. Pigment analysis showed that the pigments have been categorized within the groups of carotenoid pigments. Antioxidant activity of pigment extracts was done by measuring inhibitory concentration (IC 50 ) of pigment extract against 2,2-diphenyl-1-picrylhydrazyl (DPPH) solution. The antioxidant activity measurement of CBSCP 1-1, CBSCP 2-2, CBSCP 2-3, and CBSCP 2-4 extract, and b-carotene was 2015, 5017, 2520, 4213, and 1980 mg l -1 , respectively.

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Section: Discussionmentioning

confidence: 99%

Characterization of carotenoid pigments from bacterial symbionts of soft-coral Sarcophyton sp. from North Java Sea

Kusmita¹,

Mutiara²,

Nuryadi

et al. 2017

Int Aquat Res

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“…Simbiosis yang terjadi antara bakteri dengan spon laut menyebabkan invertebrata laut ini memiliki potensi antibakteri yang lebih besar dibandingkan dengan organisme darat dan laut lainnya [6].…”

Section: Pendahuluanunclassified

Isolasi dan Karakterisasi Senyawa Antibakteri dari Fraksi Etil Asetat Bakteri Bacillus sp.3 (A1) yang Bersimbiosis dengan Spon Laut Haliclona fascigera

2017

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Bacillus sp. 3 is one of symbiotic bacteria of marine sponge Haliclona fascigera obtained from Mandeh Island, Pesisir Selatan, West Sumatra that can produce antibacterial compounds. This study aims to isolate the antibacterial compounds produced by Bacillus sp.3 (A1). The methods that has been used were bacterial cultivation on Nutrient Broth medium using Shaker Incubator at 150 RPM at 37ºC for 48 hours and isolation of antibacterial compound. The isolation method of the compound was carried out by column chromatography method. The testing of antibacterial activity was done by using agar diffusion method. Characterization of isolated compounds were performed using UV and IR spectrophotometer. From this research, two compounds RJ1 and RJ2 were obtained. The compound RJ1 was yellow oily (2.8 mg). The compound RJ1 can inhibit Staphylococcus aureus ATCC 25923 at a 1% of Minimum Inhibition Concentration (KHM). RJ2 compound was yellow oily (3.1 mg). The RJ2 compound can inhibit Escherichia coli ATCC 25922 at a 1% of Minimum Inhibition Concentration (MIC).ABSTRAK: Bacillus sp. 3 (A1) merupakan salah satu isolat bakteri simbion spon laut Haliclona fascigera asal Pulau Mandeh, Pesisir Selatan, Sumatra Barat yang dapat memproduksi senyawa antibakteri. Penelitian ini bertujuan untuk mengisolasi senyawa aktif sebagai antibakteri yang dihasilkan oleh Bacillus sp.3 (A1). Metoda yang digunakan yaitu kultivasi bakteri pada media Nutrient Broth menggunakan Inkubator Shaker dengan kecepatan 150 RPM pada suhu 37ºC selama 48 jam dan isolasi senyawa antibakteri. Metode pemisahan senyawa dilakukan dengan metoda kromatografi kolom. Pengujian aktivitas antibakteri dlakukan dengan metoda difusi agar. Karakterisasi senyawa hasil isolasi dilakukan dengan menggunakan spektrofotometer UV dan spektrometer IR. Dari penelitian ini didapatkan dua senyawa RJ1 dan RJ2. Senyawa RJ1 berupa minyak berwarna kuning (2,8 mg). Senyawa RJ1 dapat menghambat bakteri uji Staphylococcus aureus ATCC 25923 pada Konsentrasi Hambat Minimum 1%. Senyawa RJ2 berupa minyak berwarna kuning (3,1 mg). Senyawa RJ2 dapat menghambat bakteri uji Escherichia coli ATCC 25922 pada konsentrasi hambat minimum 1%. Fakta di atas menjelaskan bahwa perlunya penemuan senyawa antibakteri atau senyawa bioaktif baru yang berasal dari sumber daya alam di Indonesia. Indonesia dikenal sebagai negara bahari dengan luas 75 % berupa lautan, 24 Keywords

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“…The role of sponge-associated microorganisms in the synthesis of compounds of biological interest is a subject of scientific debate, as in some cases these bacteria are reported to produce bioactive metabolites (Imhoff and Stöhr 2003). Antimicrobial activity has been reported for the bacteria associated with marine sponges collected from diverse areas viz., Antarctic region (Mangano et al, 2009), temperate (Chellossi et al, 2004 and tropical coastal waters (Thakur and Anil 2000, Kanagasabapathy et al, 2005, Anand et al, 2006. The present investigation aims at characterizing the cultivable heterotrophic fraction of surface-associated bacteria in a marine sponge, Stylissa sp from Andaman, India and investigating their antimicrobial properties against selected human, animal and plant pathogens.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Antibacterial Activity of Bacteria Associated with Stylissa Sp, a Marine Sponge

Krishnan¹

2014

Adv. Anim. Vet. Sci.

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Among marine invertebrates, sponges are the most prolific phylum, with regard to presence of novel pharmacologically active compounds. Bacteria, living on the surface of marine invertebrates, have been found to produce chemicals that have potential antimicrobial activities. To test this hypothesis, a total of about 30 bacterial isolates associated with the marine sponge, Stylissa sp collected from North Bay, Andaman were successfully screened for antimicrobial activity against selected human, animal and plant pathogens including Aeromonas hydrophila, Bacillus subtilis, Enterococcus durans, Streptococcus lentus, Klebsiella pneumoniae and Rolstonia solanacearum using disc diffusion assay. The bacterial isolates were identified as belonging to the genera Marinomonas, Alteromonas, Marinobacter, Chromohalobacter, Halomonas, Cytophagas and Amylocolate based on their biochemical properties. Of the total bacterial isolates tested, 66.67% were found to have antimicrobial properties ranging from broad spectrum to species specific. One of the isolates, S8 inhibited K. pneumoniae with about 95% and 30% of the efficiency of Erythromycin and Ciprofloxacin, respectively. The findings of the study highlight the importance of sponge surfaceassociated bacteria in producing new natural compounds with antimicrobial activities.All copyrights reserved to Nexus® academic publishers

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Inhibitory Activities of Surface Associated Bacteria Isolated from the Marine Sponge Pseudoceratina purpurea

Cited by 13 publications

References 31 publications

Characterization of carotenoid pigments from bacterial symbionts of soft-coral Sarcophyton sp. from North Java Sea

Characterization of carotenoid pigments from bacterial symbionts of soft-coral Sarcophyton sp. from North Java Sea

Isolasi dan Karakterisasi Senyawa Antibakteri dari Fraksi Etil Asetat Bakteri Bacillus sp.3 (A1) yang Bersimbiosis dengan Spon Laut Haliclona fascigera

Characterization of the Antibacterial Activity of Bacteria Associated with Stylissa Sp, a Marine Sponge

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