“…High-pressure methods are used to investigate protein denaturation, unfolding, conformational changes, enzyme kinetics, etc., but they also have valuable application in studying quaternary structure and equilibria of oligomeric proteins. Many oligomeric proteins have been investigated by high-pressure methods, including those of low number of subunits, mainly dimers (Paladini and Weber, 1981;Silva et al, 1986;Ruan and Weber, 1988;Erijman et al, 1993;Kornblatt et al, 2004;Marchal et al, 2012;Ingr et al, 2015) and tetramers (Jaenicke and Koberstein, 1971;Royer et al, 1986;Ruan and Weber, 1989;Pin et al, 1990;Devillebonne and Else, 1991; Ruan and Weber, 1993;Girard et al, 2010), hexamers (Foguel and Weber, 1995), higher oligomers and viral capsids (Silva and Weber, 1988;Silva et al, 1989Silva et al, , 1992Da Poian et al, 1993;Silva et al, 1996;Weber et al, 1996) or prion oligomers (Torrent et al, 2015), protein aggregates with less organized structure like casein micelles (Gebhardt et al, 2005(Gebhardt et al, , 2006(Gebhardt et al, , 2011 and even polymeric structures, e.g. TMV-virus (Bonafe et al, 1998) or microtubules and microfilaments (Messier and Seguin, 1978;Kobori et al, 1996;Nishiyama et al, 2010).…”