Inhibition of Zea mays coniferyl aldehyde dehydrogenase by daidzin: A potential approach for the investigation of lignocellulose recalcitrance

Ferro, Ana Paula; Júnior, Rogério Flores; Finger-Teixeira, Aline; Parizotto, Ângela Valderrama; Bevilaqua, Jennifer Munik; Oliveira, Dyoni Matias de; Molinari, Hugo Bruno Correa; Marchiosi, Rogério; Santos, Wanderley Dantas dos; Seixas, Flávio Augusto Vicente; Ferrarese-Filho, Osvaldo

doi:10.1016/j.procbio.2019.11.024

Cited by 13 publications

(6 citation statements)

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“…1.2.1.68) and peroxidase (e.c. 1.11.1.7) enzymes participate in different stages of lignin biosynthesis ( Calderan-Rodrigues et al, 2019 ; Ferro et al, 2020 ). As a consequence of oxidative effects, these results may suggest that rust-resistance also relies on strengthening the cell wall, while rust-susceptibility is characterized by a defect in temporal strategy that lacks an efficient response against A. psidii -specific infection.…”

Section: Discussionmentioning

confidence: 99%

Network Analysis Combining Proteomics and Metabolomics Reveals New Insights Into Early Responses of Eucalyptus grandis During Rust Infection

et al. 2021

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Eucalyptus rust is caused by the biotrophic fungus, Austropuccinia psidii, which affects commercial plantations of Eucalyptus, a major raw material for the pulp and paper industry in Brazil. In this manuscript we aimed to uncover the molecular mechanisms involved in rust resistance and susceptibility in Eucalyptus grandis. Epifluorescence microscopy was used to follow the fungus development inside the leaves of two contrasting half-sibling genotypes (rust-resistance and rust-susceptible), and also determine the comparative time-course of changes in metabolites and proteins in plants inoculated with rust. Within 24 h of complete fungal invasion, the analysis of 709 metabolomic features showed the suppression of many metabolites 6 h after inoculation (hai) in the rust-resistant genotype, with responses being induced after 12 hai. In contrast, the rust-susceptible genotype displayed more induced metabolites from 0 to 18 hai time-points, but a strong suppression occurred at 24 hai. Multivariate analyses of genotypes and time points were used to select 16 differential metabolites mostly classified as phenylpropanoid-related compounds. Applying the Weighted Gene Co-Expression Network Analysis (WGCNA), rust-resistant and rust-susceptible genotypes had, respectively, 871 and 852 proteins grouped into 5 and 6 modules, of which 5 and 4 of them were significantly correlated to the selected metabolites. Functional analyses revealed roles for photosynthesis and oxidative-dependent responses leading to temporal activity of metabolites and related enzymes after 12 hai in rust-resistance; while the initial over-accumulation of those molecules and suppression of supporting mechanisms at 12 hai caused a lack of progressive metabolite-enzyme responses after 12 hai in rust-susceptible genotype. This study provides some insights on how E. grandis plants are functionally modulated to integrate secondary metabolites and related enzymes from phenylpropanoid pathway and lead to temporal divergences of resistance and susceptibility responses to rust.

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Section: Discussionmentioning

confidence: 99%

Network Analysis Combining Proteomics and Metabolomics Reveals New Insights Into Early Responses of Eucalyptus grandis During Rust Infection

et al. 2021

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“…Genetic and biochemical studies suggest that FA can be biosynthesized via two different metabolic pathways (de Oliveira et al, 2015). In maize, two cytosolic aldehyde dehydrogenases, REF2C and REF2D, catalyse the oxidation of coniferaldehyde to FA (Ferro et al, 2020; Končitíková et al, 2015; Nair et al, 2004). Alternatively, FA might also be produced from caffeic acid via 3‐ O ‐methylation by COMT (Fornalé et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Hydroxycinnamaldehyde dehydrogenase (HCALDH) activity was carried out as described by Nair, Bastress, Ruegger, Denault, and Chapple (2004) and Ferro et al (2020). The extraction buffer for HCALDH activity contained 50 mM Hepes‐HCl (pH 8.0), 5 mM DTT, 1 mM EDTA and 10% glycerol ( vol/vol ).…”

Section: Methodsmentioning

confidence: 99%

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Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Oliveira

Mota

Salatta

et al. 2020

Plant Cell & Environment

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Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored. Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants. Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems. The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots. Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants. Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots. Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis. The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3-and 4-O-feruloyl

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“…Sibout et al (2002) reported that ferulate-5-hydroxylase has a role in S-lignin deposition and its overexpression enhanced the S-lignin level in Arabidopsis thaliana [56]. Coniferyl aldehyde dehydrogenase, was involved in the biosynthesis of ferulic acid in Zea mays, an essential structural component in the cell wall and increase its strength and rigidity [57]. Two other genes, cinnamoyl-coA reductase and peroxidase were also found to be downregulated in both the blanched and the control samples.…”

Section: Discussionmentioning

confidence: 97%

Influence of Blanching on the Gene Expression Profile of Phenylpropanoid, Flavonoid and Vitamin Biosynthesis, and Their Accumulation in Oenanthe javanica

Kumar

Huang

et al. 2022

Antioxidants

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Field blanching is a process used in agriculture to obtain sweet, delicious, and tender stems of water dropwort by obstructing sunlight. The nutritional and transcriptomic profiling of blanched water dropwort has been investigated in our previous studies. However, the effect of blanching on the production of secondary metabolites and different vitamins in water dropwort has not been investigated at the transcriptomic level. This study explored the transcriptomic variations in the phenylpropanoid biosynthesis, flavonoid biosynthesis, and different vitamin biosynthesis pathways under different blanching periods in the water dropwort stems (pre-blanching, mid-blanching, post-blanching, and control). The results show that polyphenol and flavonoid contents decreased; however, the contents of vitamins (A, B1, B2, and C) and antioxidant activity increased significantly after blanching. Furthermore, the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of blanched water dropwort showed the downregulation of many important genes involved in phenylpropanoid and flavonoid biosynthesis pathways, and the downregulation of these genes might be the reason for the reduction in polyphenol and flavonoid contents. We also examined and highlighted the genes involved in the higher vitamin content, antioxidant activity, pale color, tenderness, and sweetness of the blanched stem of water dropwort. In conclusion, the present study explored the role of phenylpropanoid and vitamin biosynthesis, and it will provide a basis for future investigation and application in the blanch cultivation of water dropwort.

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Inhibition of Zea mays coniferyl aldehyde dehydrogenase by daidzin: A potential approach for the investigation of lignocellulose recalcitrance

Cited by 13 publications

References 55 publications

Network Analysis Combining Proteomics and Metabolomics Reveals New Insights Into Early Responses of Eucalyptus grandis During Rust Infection

Network Analysis Combining Proteomics and Metabolomics Reveals New Insights Into Early Responses of Eucalyptus grandis During Rust Infection

Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Influence of Blanching on the Gene Expression Profile of Phenylpropanoid, Flavonoid and Vitamin Biosynthesis, and Their Accumulation in Oenanthe javanica

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