Inhibition of Vascular Permeability Increase in Mice

Inagaki, Nobuya; Miura, Tetsuji; Nagai, Hiroichi; Ono, Yutaka; Koda, Akihide

doi:10.1159/000234681

Cited by 22 publications

(3 citation statements)

References 13 publications

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“…Preparation for Anti-OVA Serum The procedure employed was a slight modification of the method of Inagaki et al 15) Ovalbumin was suspended in 20 mg/ml Al(OH) 3 solution (final concentration of OVA, 10 mg/ml), and 0.2 ml of this suspension (OVA, 2 mg) was intraperitoneally injected into 4-week-old female Balb/c mice (Japan SLC, Hamamatsu, Japan) 5 times at 2 week intervals. Ten days after the 5th injection of OVA, whole blood was collected to obtain anti-OVA serum.…”

Section: Methodsmentioning

confidence: 99%

Effect of Oral Treatment of Perilla frutescens and Its Constituents on Type-I Allergy in Mice.

Makino

Furuta

Fujii

et al. 2001

Biological & Pharmaceutical Bulletin

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Perilla frutescens Britton (perilla, Labiatae) is a medicinal herb prescribed in Saiboku-to [Japanese letters: see text], which is a Kampo formula effective for allergic diseases such as bronchial asthma. The present study was conducted to evaluate the anti-allergic effect of orally administered perilla decoction and to identify the active constituents using mice ear-passive cutaneous anaphylaxis (PCA)-reaction, which is one of the animal models for type I allergy. Perilla decoction significantly suppressed PCA-reaction, and the inhibition % at the dose of 500 mg/kg was 43%. The perilla decoction contains 5.3% of luteolin 7-O-[beta-glucuronosyl(2-->1)beta-glucuronide], 1.6% of apigenin 7-O-[beta-glucuronosyl(2-->1)beta-glucuronide], 0.49% of scutellarin, and 2.5% of rosmarinic acid (weight of compound/dried weight of perilla decoction %), respectively. When these constituents were orally administered to mice at the dose equivalent to 500 mg/kg of perilla decoction, rosmarinic acid and apigenin 7-O-[beta-glucuronosyl(2-->1)beta-glucuronide] significantly suppressed PCA-reaction, and their inhibition % was 41% (p<0.01) and 32% (p<0.05), respectively. Since the inhibition % or perilla decoction and rosmarinic acid were nearly equal, the anti-allergic effect of perilla decoction depends primarily on rosmarinic acid. The standard Saiboku-to decoction contained 0.013% of rosmarinic acid, which was too low to exhibit anti-allergic activity in a daily dose of Saiboku-to in adults, suggesting that perilla would be prescribed in Saiboku-to to exhibit other pharmacological effects than its anti-allergic activity, such as a sedative.

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Section: Methodsmentioning

confidence: 99%

Effect of Oral Treatment of Perilla frutescens and Its Constituents on Type-I Allergy in Mice.

Makino

Furuta

Fujii

et al. 2001

Biological & Pharmaceutical Bulletin

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“…The vascular effect of prednisolone may also participate in its inhibition of swelling. 17) The epidermal permeability barrier is made up of highly hydrophobic lipids, which form multiple membrane bilayers in the outer cornified cell layers. [2][3][4] Patients with atopic dermatitis often exhibit dry skin that may correlate with a defect in the epidermal barrier structure.…”

Section: Discussionmentioning

confidence: 99%

Effects of Prednisolone on the Cutaneous Reaction and Skin Barrier Function in Mice Treated with a Hapten

Ueda

Sone

Inagaki

et al. 2003

Biological & Pharmaceutical Bulletin

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Glucocorticoids are effective drugs for the treatment of allergic skin diseases. In the present study, we observed the effects of prednisolone on the cutaneous reaction and skin barrier function in mice treated with a hapten, 2,4-dinitrofluorobenzene. Repeated hapten application onto the mouse ear resulted in a potent ear swelling with an elevation of specific serum IgE. The ear swelling appeared following the second application of the hapten and peaked at 24 h after each application. Specific serum IgE was detected first after the fourth hapten application. Topical treatment with prednisolone apparently suppressed the swelling, whereas it failed to affect the serum specific IgE level. The hapten application caused an increase in transepidermal water loss, which was potently inhibited by prednisolone, although the water content was not affected. Amounts of triglyceride and cholesterol in the ear skin increased after repeated hapten applications, whereas the relative amount of free fatty acid and ceramide diminished. Prednisolone exhibited an inhibitory effect on the changes in lipid content. Thus prednisolone apparently inhibits the alteration of skin barrier function caused by hapten application as well as the cutaneous reaction.

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“…Corticosteroids are potent inhibitors of inflammatory oedema and this probably represents a major component of their therapeutic activity in inflammatory diseases. Corticosteroids, indeed, inhibit skin reactions, caused by vascular permeability increasing factors, including IgE antibody-mediated homologous passive cutaneous anaphylaxis (Inagaki et al, 1988). In rat tracheobronchial mucosa, corticosteroids failed to reduce local oedema induced by cigarette smoke and by instillation of hydrochloric acid and gastric juice Martling & Lundberg, 1988).…”

Section: Discussionmentioning

confidence: 99%

Increase in vascular permeability produced in rat airways by PAF: potentiation by adrenalectomy

Boschetto

Musajo

Tognetto

et al. 1992

British J Pharmacology

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and tNational Heart and Lung Institute, London 1 The effect of bilateral adrenalectomy on the sensitivity of blood vessels in rat airways to mediators that increase vascular permeability was examined. 2 An increase in vascular permeability was induced by intravenous platelet activating factor (PAF, 50, 100, 500, 1000 ng kg-1) and measured by quantifying the extravasation of Evans blue dye. 3 PAF consistently increased the amount of Evans blue extravasation in the larynx, trachea, main bronchi and intrapulmonary airways in sham-operated rats. 4 The magnitude of this extravasation was significantly greater in the larynx (P < 0.05), trachea (P < 0.05) and main bronchi (P < 0.05) of the adrenalectomized rats than it was in these tissues of the sham-operated rats. 5 When adrenalectomized rats were given subcutaneous dexamethasone (0.2mgkg-1 4h before PAF) the amount of plasma extravasation produced by PAF was decreased to the level of the sham-operated rats.6 We conclude that adrenalectomy potentiates the increase in airway vascular permeability induced by PAF in rats and that this effect may be due to the depletion of endogenous corticosteroids.

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Inhibition of Vascular Permeability Increase in Mice

Cited by 22 publications

References 13 publications

Effect of Oral Treatment of Perilla frutescens and Its Constituents on Type-I Allergy in Mice.

Effect of Oral Treatment of Perilla frutescens and Its Constituents on Type-I Allergy in Mice.

Effects of Prednisolone on the Cutaneous Reaction and Skin Barrier Function in Mice Treated with a Hapten

Increase in vascular permeability produced in rat airways by PAF: potentiation by adrenalectomy

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