Inhibition of TNF-α protects against hepatic ischemia–reperfusion injury in rats via NF-κB dependent pathway

Abstract

Hepatic ischemia-reperfusion injury (I/R) is a serious health problem associated with liver transplantation, resection surgery, and various types of shock especially hemorrhagic shock. In the present investigation, the effect of inhibition of tumor necrosis factor-alpha (TNF-α) using pentoxifylline or infliximab against hepatic I/R injury induced in rats by 45-min ischemia and 1-h reperfusion was studied. It was observed that both pentoxifylline and infliximab-treated groups showed a significantly lower extent and severity of liver injury. This is attributed to (1) a decrease in oxidative stress markers, (2) reduction of the expression of TNF-α, TNF-α type-1 receptors, and nuclear factor kappa B (NF-κB). Thus TNF-α inhibition may be one of the therapeutic interventions to overcome the deleterious effects of I/R on liver via reduction of oxidative stress and inhibition of inflammatory cascade.

Infliximab Modulates Cisplatin-Induced Hepatotoxicity in Rats

“…33, No. 5,2016 to a decrease in tumor growth. However, the mechanism of action of both drugs on these enzymes is unclear.…”

“…The animals in the Cis group received a single-dose injection of Cis (Cisplatinum Ebewe, 0.5 mg/mL) (7 mg/kg), administered intraperitoneally, and were sacrificed five days later (14). A single dose of Ib (Remicade®; Schering plough, USA) (7 mg/kg) was given to the CIN group rats 72 h prior to Cis injection (5). A single dose of cisplatin (Koçak Pharma; İstanbul, Turkey) (7 mg/kg) was given after 72 h and the animals were killed five days after this injection.…”

“…33, No. 5,2016 Tissue TNF-α TNF-α concentration was evaluated using a commercially existent rat TNF-α enzyme-linked immunosorbent assay (ELISA) kit (eBioscience; Vienna, Austria). The absorbance was evaluated by the ELISA plate reader at a wavelength of 450 nm.…”

Publication trends in Naunyn-Schmiedeberg’s Archives of Pharmacology: focus on pharmacology in Egypt

“…In contrast, the papers coming from Islamic countries typically have not focused on natural products, but rather on chemically defined substances. Moreover, they represent a broad range of topics such as ischemia/reperfusion injury (Hosseinzadeh et al 2012; M. M. El-Mas : H. M. El-Gowelli Mahmoud et al 2012), atrial fibrillation (Khori et al 2012), allergic airway disease (Nader et al 2012), skeletal muscle (Hosseinzadeh et al 2012;Renno et al 2012), urinary bladder smooth muscle (Balkanci et al 2012), or the metabolism of xenobiotics (Pang et al 2012).…”

Efeitos da administração endovenosa de pentoxifilina na isquemia-reperfusão pancreática: estudo experimental em ratos

“…Com base nestes efeitos, alguns autores estudaram a ação da PTX em modelos experimentais de I/R de órgãos abdominais, mostrando um efeito protetor da droga na síndrome da resposta inflamatória sistêmica, evidenciado por menor produção TNF-α e diminuição do estresse oxidativo 48,49,50,51 . De fato, em um estudo, a PTX reduziu a produção de TNF-α, o edema pulmonar e a peroxidação lipídica na I/R intestinal em ratos 51 .…”