Inhibition of thrombin-induced signaling by resveratrol and quercetin: effects on adenosine nucleotide metabolism in endothelial cells and platelet–neutrophil interactions

Kaneider, Nicole C.; Mosheimer, Birgit A.; Reinisch, Norbert; Patsch, Josef R.; Wiedermann, Christian J.

doi:10.1016/j.thromres.2004.06.020

Cited by 55 publications

(41 citation statements)

References 38 publications

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“…Schmatz et al also observed increased platelet ATPDase activity in streptozotocin-diabetic rats in vitro, after treatment with resveratrol, gallic and caffeic acid, but unlike Kaneider et al, they noticed that quercetin and rutin may diminish ATPDase activity [41,42]. In line with our studies, Ashraf et al note that methanol extract obtained from Cymbopogan jawarancusa, Asparagus officinalis, Tribulus terrestris, Rubia cordizolla, Echimops echimatus and Portulaca oleracea also exerted an inhibitory action on ATPDase obtained from chicken liver [43].…”

Section: Discussionmentioning

confidence: 95%

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CD39/NTPDase-1 expression and activity in human umbilical vein endothelial cells are differentially regulated by leaf extracts from Rubus caesius and Rubus idaeus

Dudzińska

Luzak

Boncler

et al. 2014

Cellular and Molecular Biology Letters

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Many experimental studies have demonstrated the favorable biological activities of plants belonging to the genus Rubus, but little is known of the role of Rubus leaf extracts in the modulation of the surface membrane expression and activity of endothelial apyrase. The aim of this study was to assess the influence of 1–15 μg/ml Rubus extracts on CD39 expression and enzymatic activity, and on the activation (ICAM-1 expression) and viability of human umbilical vein endothelial cells (HUVEC). The polyphenolic contents and antioxidative capacities of extracts from dewberry (R. caesius L.) and raspberry (R. idaeus L.) leaves were also investigated. The techniques applied were flow cytometry (endothelial surface membrane expression of ICAM-1 and CD39), malachite green assay (CD39 activity), HPLC-DAD (quantitative analysis of polyphenolic extract), ABTS, DPPH and FRAP spectrometric assays (antioxidant capacity), and the MTT test (cell viability). Significantly increased CD39 expressions and significantly decreased ATPDase activities were found in the cells treated with 15 μg/ml of either extract compared to the results for the controls. Neither of the extracts affected cell proliferation, but both significantly augmented endothelial cell ICAM-1 expression. The overall antioxidant capacities of the examined extracts remained relatively high and corresponded well to the determined total polyphenol contents. Overall, the results indicate that under in vitro conditions dewberry and raspberry leaf extracts have unfavorable impact on endothelial cells.

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Section: Discussionmentioning

confidence: 95%

“…The influence of polyphenolic compounds isolated from various plants on the expression and enzymatic activity of CD39 was described earlier. Kaneider et al report that thrombin was found to downregulate human endothelial apyrase activity, which was then restored by incubation with resveratrol and quercetin [41].…”

Section: Discussionmentioning

confidence: 99%

CD39/NTPDase-1 expression and activity in human umbilical vein endothelial cells are differentially regulated by leaf extracts from Rubus caesius and Rubus idaeus

Dudzińska

Luzak

Boncler

et al. 2014

Cellular and Molecular Biology Letters

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“…Anti-inflammatory activity of resveratrol and some of its proposed mechanisms of action were attributed mostly in inhibition of COX activity (Das & Das, 2007;Kundu et al, 2006). Resveratrol also interferes with the pro-inflammatory signalling of thrombin resulting in the inhibition of adenosine nucleotide secretion from activated platelets and decreased neutrophil functions via inhibition of PAP and P2-receptor signalling through MAPK and cJun and JNK (Kaneider et al, 2004). However there is amply evidence about the modulatory effect of resveratrol on MAPK pathways, the data are so cell type and experimental system specific that it is hard to conclude and draw a coherent picture.…”

Section: Signaling Pathwaysmentioning

confidence: 99%

Anti-Inflammatory Role of Natural Polyphenols and Their Degradation Products

Veres¹

2012

Severe Sepsis and Septic Shock - Understanding a Serious Killer

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“…68 Moreover, several phenolics such as resveratrol, quercetin and methylgallic acid (a major metabolite of gallic acid) and gallates (gallic acid esters) inhibit TNF-α and/or thrombin and/or IL-1 activation of endothelial cells. [69][70][71][72] In turn, this effect could result to reduce PAF levels since TNF-α, IL-1, and thrombin induce endothelial PAF production. 73 Flavonoid, quercetin luteolin, and apigenin prevent endothelial expression of inducible adhesion molecules and endothelial uptake of ox-LDL.…”

Section: Ldl Oxidationmentioning

confidence: 99%

Lipid minor constituents in wines. A biochemical approach in the French paradox

Fragopoulou¹,

Demopoulos²,

Antonopoulou

2009

IJWR

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Abstract:The "French paradox" is the observation that the French suffer a relatively low incidence of coronary heart disease, despite having a diet relatively rich in saturated fats. Several theories have been proposed in order to explain this phenomenon and several debates arose. One of them attributed this phenomenon to the regular and moderate consumption of wine in France. More specifi c, it is thought that the existence of bioactive compounds in wine could have an effect on the cardiovascular system, preventing or delaying atherosclerosis. The mechanisms mediating these benefi cial effects include: low-density lipoprotein oxidation; endothelium function; smooth muscle cells proliferation; platelet aggregation and angiogenesis. Several mediators participate in these pathophysiological mechanisms, among them are plateletactivating factor (PAF) and oxidized phospholipids that play a crucial and essential role in the initiation and the progression of atherogenesis. In this review, apart from the already known and well characterized biological effects of wine bioactive compounds, the co-existence of compounds that could modulate the production and the actions of PAF is highlighted. The existence of bioactive compounds in wine that could reduce PAF production and inhibit its actions may offer a new insight into the well known French paradox and expand the already reported mechanisms by including the inhibition of PAF actions.

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Inhibition of thrombin-induced signaling by resveratrol and quercetin: effects on adenosine nucleotide metabolism in endothelial cells and platelet–neutrophil interactions

Cited by 55 publications

References 38 publications

CD39/NTPDase-1 expression and activity in human umbilical vein endothelial cells are differentially regulated by leaf extracts from Rubus caesius and Rubus idaeus

CD39/NTPDase-1 expression and activity in human umbilical vein endothelial cells are differentially regulated by leaf extracts from Rubus caesius and Rubus idaeus

Anti-Inflammatory Role of Natural Polyphenols and Their Degradation Products

Lipid minor constituents in wines. A biochemical approach in the French paradox

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