Inhibition of the synthesis of eicosanoid-like substances in a human oral cancer cell line by interferon-γ and eicosapentaenoic Acid

Meng, Ching-Liang; Yang, Cheng‐Yu; Shen, Kuo‐Liang; Wong, P Y; Lee, H.K.

doi:10.1016/s0003-9969(98)00078-8

Cited by 10 publications

(7 citation statements)

References 37 publications

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“…The OEC-M1 cells, derived from a human oral epidermoid carcinoma, were established and provided by Dr. Ching-Liang Meng [ 32 , 33 ]. OEC-M1 cells were maintained in RPMI1640 (Gibco) supplemented with 10% fetal bovine serum, 100 U/mL of penicillin, and 100 μg/mL of streptomycin at 37°C in an atmosphere comprising 95% air and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Autophagy-Regulated ROS from Xanthine Oxidase Acts as an Early Effector for Triggering Late Mitochondria-Dependent Apoptosis in Cathepsin S-Targeted Tumor Cells

et al. 2015

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Cathepsin S (CTSS), which is highly expressed in various malignant tumor cells, has been proposed to promote tumor progression, migration, and invasion. CTSS inhibition not only blocks tumor cell invasion and endothelial tube formation but also induces cellular cytotoxicity. In our previous studies, we have observed that CTSS inhibition induces autophagy, which is responsible for up-regulating xanthine oxidase for early ROS generation and consequent cell death. However, whether the autophagy-regulated early ROS triggers apoptosis remains unclear. We conducted a long-term follow-up study to investigate the relationship between early autophagy and late mitochondria-dependent apoptosis. We demonstrated that early ROS generation is critical for mitochondria damage and the activation of intrinsic apoptotic pathway. Attenuating the early ROS level diminished later mitochondrial damage and downstream apoptotic signaling. Collectively, mitochondria-dependent apoptosis is regulated by autophagy-regulated early ROS, which serves as an early effector that triggers mitochondrial signaling for late apoptosis. The data emphasize the essential role of autophagy-regulated early ROS in triggering late apoptotic signaling.

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Section: Methodsmentioning

confidence: 99%

Autophagy-Regulated ROS from Xanthine Oxidase Acts as an Early Effector for Triggering Late Mitochondria-Dependent Apoptosis in Cathepsin S-Targeted Tumor Cells

et al. 2015

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“…It was cultured in Dulbecco's modified Eagle's minimal essential medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS), 100 units/ml penicillin and 100 mg/ml streptomycin, in 25‐cm 2 flasks. The oral epithelioid carcinoma cell, Meng‐1 (OECM‐1), was a generous gift from Dr Ching‐Liang Meng (National Defense Medical Center, Taiwan) . OECM‐1 cells were cultured in RPMI‐1640 medium (Invitrogen) containing 10% FBS, 100 units/ml penicillin and 100 mg/ml streptomycin.…”

Section: Methodsmentioning

confidence: 99%

Expression of matriptase correlates with tumour progression and clinical prognosis in oral squamous cell carcinoma

et al. 2014

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“…OC3 cells were maintained in DMEM in addition to a twofold volume of keratinocyte-SFM mixed medium supplemented with 24 mM of NaHCO 3 , 25 mM of HEPES, 100 ppm of penicillin, 100 ppm of streptomycin, and 10% v/v heat-inactivated fetal bovine serum; pH7.4. OEC-M1 cells were maintained in Roswell Park Memorial Institute 1640 medium supplemented with 24 mM of NaHCO 3 , 25 mM of HEPES, 10,000 U of penicillin, 10,000 U of streptomycin, and 10% heat-inactivated fetal bovine serum; and pH 7.421 FaDu cells were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 0.1% penicillin/streptomycin. Cells were incubated in a humidified atmosphere containing 95% air and 5% CO2 at 37°C 20,21…”

Section: Methodsmentioning

confidence: 99%

Cordycepin enhances cisplatin apoptotic effect through caspase/MAPK pathways in human head and neck tumor cells

Chen¹,

Wang²,

Pan³

et al. 2013

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PurposeThe present study aims to investigate whether the combination treatment of cordycepin (an extracted pure compound from Cordyceps sinensis) and cisplatin (a platinum-based chemotherapy drug) has better apoptotic effect in head and neck squamous cell carcinoma (HNSCC).MethodsThe apoptotic influences of cordycepin and/or cisplatin treatments to human OC3, OEC-M1, and FaDu HNSCC cells were investigated by morphological observations, viability assay, flow cytometry assay, and Western blotting methods.ResultsData showed that the cell death phenomenon increased as the dosage of cordycepin or cisplatin increased, and it appeared more in cordycepin plus cisplatin cotreatment among three cell lines. Cell survival rates significantly decreased as the dosage of cordycepin or cisplatin increased, and the better apoptotic effects were observed in cotreatment. Cell cycle analysis further demonstrated that percentages of subG1 cells in cordycepin or cisplatin treatments significantly increased, suggesting that cells underwent apoptosis, and cordycepin plus cisplatin induced many more subG1 cells. Furthermore, cordycepin or cisplatin induced caspase-8, caspase-9, caspase-3, and poly adenosine diphosphate-ribose polymerase protein cleavages, and stimulated c-Jun NH2-terminal kinase, extracellular signal-regulated kinase, and p38 protein phosphorylations. Moreover, cordycepin plus cisplatin cotreatment significantly activated those proteins with much better effects among three cell lines.ConclusionCordycepin plus cisplatin have better apoptotic effect by activating caspase activation with possible MAPK pathway involvement in HNSCC cells.

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Inhibition of the synthesis of eicosanoid-like substances in a human oral cancer cell line by interferon-γ and eicosapentaenoic Acid

Cited by 10 publications

References 37 publications

Autophagy-Regulated ROS from Xanthine Oxidase Acts as an Early Effector for Triggering Late Mitochondria-Dependent Apoptosis in Cathepsin S-Targeted Tumor Cells

Autophagy-Regulated ROS from Xanthine Oxidase Acts as an Early Effector for Triggering Late Mitochondria-Dependent Apoptosis in Cathepsin S-Targeted Tumor Cells

Expression of matriptase correlates with tumour progression and clinical prognosis in oral squamous cell carcinoma

Cordycepin enhances cisplatin apoptotic effect through caspase/MAPK pathways in human head and neck tumor cells

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