Inhibition of the sea urchin sperm acrosome reaction by a lignin-derived macromolecule

Pillai, Muralidharan C.; Blethrow, H.; Higashi, Richard M.; Vines, Carol A.; Cherr, Gary N.

doi:10.1016/s0166-445x(96)00821-1

Cited by 13 publications

(4 citation statements)

References 26 publications

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“…Fertilisation occurs in the water column in marine broadcast spawners. External stressors, such as pollutants, could affect sperm function (and subsequent fertilisation success) by, amongst other things, the spontaneous generation of reactive oxygen species (Kazama et al, 2014), inducing sperm DNA damage (Lewis and Galloway, 2009), disrupting sperm swimming ability (Caldwell et al, 2004), and the ability of the sperm to undergo the acrosome reaction (Pillai et al, 1997). Sperm limitation and population dynamics are related in broadcast spawners (Levitan and Petersen, 1995).…”

Section: Discussionmentioning

confidence: 99%

Assessing the impact of diclofenac, ibuprofen and sildenafil citrate (Viagra®) on the fertilisation biology of broadcast spawning marine invertebrates

Zanuri

Bentley

Caldwell

2017

Marine Environmental Research

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Exposure to synthetic chemicals is a key environmental challenge faced by aquatic organisms. The time and dose effects of the pharmaceuticals diclofenac, ibuprofen, and sildenafil citrate on sperm motility and successful fertilisation are studied using the echinoderms, Asterias rubens and Psammechinus miliaris, and the polychaete worm Arenicola marina, all important components of the marine benthos. Motility was reduced for all species when exposed to diclofenac concentrations ≥0.1 μg/L. Exposure to ≥1.0 μg/L of ibuprofen affected only P. miliaris gametes and fertilisation success of A. marina. A. rubens and P. miliaris sperm increased in both percentage motility and swimming velocity when exposed to sildenafil citrate at concentrations ≥18 and ≥ 50 ng/L, respectively. Pre-incubation of sperm with sildenafil citrate significantly increased fertilisation success in A. rubens and P. miliaris but not in A. marina. Pre-incubated A. rubens oocytes fertilised successfully in ibuprofen. According to EU Directive 93/67/EEC, diclofenac is classified as a very toxic substance to gametes of A. rubens, P. miliaris, and A. marina (EC = 100-1000 μg/L) while ibuprofen is classified as very toxic to gametes of P. miliaris but non-toxic to gametes of A. marina (EC > 10,000 μg/L). The present study indicates that diclofenac exposure may have negative impacts on invertebrate reproductive success, whereas ibuprofen potentially may compromise P. miliaris reproduction. This study provides a valuable insight into the mechanisms that allow marine invertebrates to survive and reproduce in contaminated and changing habitats.

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Section: Discussionmentioning

confidence: 99%

Assessing the impact of diclofenac, ibuprofen and sildenafil citrate (Viagra®) on the fertilisation biology of broadcast spawning marine invertebrates

Zanuri

Bentley

Caldwell

2017

Marine Environmental Research

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“…The extent, orientation, and distribution of the sulfation charge along anionic polymers are believed to be determining factors in their relative affinities during complementary docking to ligands (Deangelis and Glabe, 1990;Kim et al, 1997). Our previous studies on an LDM related to LSA have shown that it inhibits the sea urchin sperm acrosome reaction (Pillai et al, 1997), and we have preliminary evidence that LSA competes with the natural sulfated ligand, egg jelly, on the sperm surface (Salinas and Cherr, 2000). Furthermore, we have observed that LSA binds to the head of capacitated macaque sperm (Figure 4, B2 and D2), a location consistent with its biological activity.…”

Section: Discussionmentioning

confidence: 85%

“…For example, an LDM has been shown to dramatically inhibit the growth of fibroblast and sarcoma cells (Sorimachi, 1992), but yet activate murine macrophages and induce proliferation of bone marrow cells (Suzuki et al, 1989a). LDMs have also been shown to inhibit fertilization in echinoderms without showing cytotoxic effects (Higashi et al, 1992;Cherr et al, 1993) through a mechanism that involves inhibition of the sperm acrosome reaction (Pillai et al, 1997). Another polysulfonated compound has recently been shown to have sperm-mediated inhibition of fertilization in nonprimate species and is proposed as a potential human contraceptive (Anderson, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…LDMs have been shown to inhibit fertilization in a number of nonmammalian species without being cytotoxic to gametes (Higashi et al, 1992;Cherr et al, 1993;Pillai et al, 1997). LSA is virtually nontoxic when administered orally (LD 50 Ͼ 40 g/kg) and has been used for many years as an animal feed additive because of its antipepsin activity and the protection it provides against the development of gastric ulcers Alphin, 1968, 1969;Luscombe and Nicholls, 1973).…”

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confidence: 99%

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Lignosulfonic Acid Blocks In Vitro Fertilization of Macaque Oocytes When Sperm Are Treated Either Before or After Capacitation

TOLLNER,

OVERSTREET,

et al. 2002

Journal of Andrology

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Lignin‐derived macromolecules (LDMs) are biologically active compounds that affect a variety of cell‐to‐cell interactions including the inhibition of fertilization and embryo development in a number of nonmammalian species. The effect of lignosulfonic acid (LSA), a highly sulfonated LDM, on cynomolgus macaque sperm‐oocyte interaction was evaluated with a zona pellucida binding assay and by in vitro fertilization (IVF). Sperm were treated with LSA (1.5 mg/mL) either before washing or after capacitation. Capacitation included centrifugation through 80% Percoll followed by 2 consecutive washes with medium, overnight incubation, and activation with dibutyryl cyclic adenosine monophosphate and caffeine. The zona binding assay was performed using immature oocytes that had adhered to the center of glass “binding chambers.” The number of capacitated sperm that attached to the zona over a 3‐minute period was recorded. Sperm attachment was significantly inhibited by LSA as compared to controls whether treatment occurred after capacitation (92.5%; P < .001) or before washing (82.5%; P < .001). When sperm were treated similarly with fucoidin, a sulfated polysaccharide known to inhibit sperm‐oocyte interaction, sperm‐zona binding was significantly inhibited by postcapacitation treatment but not by prewash treatment. Treatment of sperm with LSA consistently blocked fertilization over 4 IVF cycles both before washing and after capacitation. Fertilization rate for controls was 65% ± 17%. No LSA‐treated sperm were observed on the surface of lightly rinsed oocytes after 4 hours of coincubation. Localization of biotinylated LSA showed labeling over the entire sperm surface with the greatest intensity observed over the head and midpiece. LSA treatment had no effect on the percentage of motile sperm or quality of sperm motility. Due to the antifertility properties of this nontoxic molecule, LSA appears to have potential as a vaginal contraceptive.

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Effects-Directed Studies of Pulp and Paper Mill Effluents

Hewitt

2011

The Handbook of Environmental Chemistry

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Inhibition of the sea urchin sperm acrosome reaction by a lignin-derived macromolecule

Cited by 13 publications

References 26 publications

Assessing the impact of diclofenac, ibuprofen and sildenafil citrate (Viagra®) on the fertilisation biology of broadcast spawning marine invertebrates

Assessing the impact of diclofenac, ibuprofen and sildenafil citrate (Viagra®) on the fertilisation biology of broadcast spawning marine invertebrates

Lignosulfonic Acid Blocks In Vitro Fertilization of Macaque Oocytes When Sperm Are Treated Either Before or After Capacitation

Effects-Directed Studies of Pulp and Paper Mill Effluents

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