Inhibition of the Growth of Premalignant and Malignant Human Oral Cell Lines by Extracts and Components of Black Raspberries

Han, Chunhua; Ding, Hu; Casto, Bruce C.; Stoner, Gary D.; D’Ambrosio, Steven M.

doi:10.1207/s15327914nc5102_11

Cited by 92 publications

(80 citation statements)

References 38 publications

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“…Only the alcohol fraction showed a dose-dependent decrease in ability of benzo[a]pyrene (B [a]P)-to induce transformation of Syrian hamster embryo (SHE) cells [42]. Similarly, Han, et al [43] found that the dichloromethane and alcohol fractions, and two known components; namely, ß-sitosterol and ferulic acid, selectively inhibited the growth of premalignant and malignant human oral cavity cell lines but not normal oral cells. They further found reduced levels of cyclin A and cell division cycle gene 2 (cdc2) in the premalignant cells, and reduced levels of cyclin B1, D1 and cdc2 in the malignant cells.…”

Section: In Vitro Studies With Berry Extractsmentioning

confidence: 98%

Cancer prevention with freeze-dried berries and berry components

Stoner

Wang

Zikri

et al. 2007

Seminars in Cancer Biology

150

119

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Our laboratory is developing a food-based approach to the prevention of esophageal and colon cancer utilizing freeze-dried berries and berry extracts. Dietary freeze-dried berries were shown to inhibit chemically-induced cancer of the rodent esophagus by 30-60% and of the colon by up-to 80%. The berries are effective at both the initiation and promotion/progression stages of tumor development. Berries inhibit tumor initiation events by influencing carcinogen metabolism, resulting in reduced levels of carcinogen-induced DNA damage. They inhibit promotion/progression events by reducing the growth rate of premalignant cells, promoting apoptosis, reducing parameters of tissue inflammation and inhibiting angiogenesis. On a molecular level, berries modulate the expression of genes involved with proliferation, apoptosis, inflammation and angiogenesis. We have recently initiated clinical trials; results from a toxicity study indicated that freeze-dried black raspberries are well tolerated in humans when administered orally for 7 days at a dose of 45 grams per day. Several Phase IIa clinical trials are underway in patients at high risk for esophagus and colon cancer; i.e., Barrett's esophagus, esophageal dysplasia and colonic polyps, to determine if berries will modulate various histological and molecular biomarkers of development of these diseases.

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Section: In Vitro Studies With Berry Extractsmentioning

confidence: 98%

Cancer prevention with freeze-dried berries and berry components

Stoner

Wang

Zikri

et al. 2007

Seminars in Cancer Biology

150

119

View full text Add to dashboard Cite

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“…In a number of comparative studies with 'normal' cell lines some researchers have suggested that berry components may be selectively cytotoxic against cancer cells. Black raspberry extracts exerted a distinct differential effect on pre-malignant and malignant oral epithelial cells as compared to normal human oral epithelial cells at doses of 100-200 μg/ml (20). Cocoa extracts had a differential effect on prostate cancer cell lines DU145 and 22Rv1 compared to a normal prostate epithelial cell line (21).…”

Section: Introductionmentioning

confidence: 95%

“…Liesveld et al (48) found that quercetin showed no differential cytotoxic effects against normal CD34 + haematopoietic progenitor cells or acute myeloid leukemic cells (20). Sub-fractions of black raspberry extracts (20), probably enriched in anthocyanins, were selectively cytotoxic against pre-malignant and malignant oral cell lines but not against normal oral cell lines. However, another more hydrophobic sub-fraction, probably enriched in ellagitannins, was equally cytotoxic to normal, premalignant and malignant cells.…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

“…However, another more hydrophobic sub-fraction, probably enriched in ellagitannins, was equally cytotoxic to normal, premalignant and malignant cells. In addition, ellagic acid was more cytotoxic against normal than the pre-malignant and malignant oral cell lines (20). Red wine polyphenols were equally cytotoxic against normal human mammary epithelial cells and the tumourigenic breast epithelial cell line MCF-7 (49) although sub-fractions of unknown composition showed some selectivity towards the tumourigenic cell line (22,49).…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

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Strawberry polyphenols are equally cytotoxic to tumourigenic and normal human breast and prostate cell lines

Weaver

Briscoe²,

Hou³

et al. 2009

Int J Oncol

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Abstract. The cytotoxic effects of strawberry polyphenols were investigated on normal cells and tumour cells derived from the same patient. A human prostate epithelial cell line (P21) and two tumour cell lines (P21 tumour cell line 1 and 2) derived from the same patient, and a normal human breast epithelial cell line (B42) and a tumour line derived from it (B42 clone 16) were used. A polyphenol-rich extract derived from strawberry or anthocyanin or tannin-rich sub-fractions were applied to the cell lines in doses varying from 50 to 1.5 μg/ml. The strawberry extract was cytotoxic with doses of ~5 μg/ml causing a 50% reduction in cell survival in both the normal and the tumour lines. The extracts were also cytotoxic to peripheral blood human lymphocytes stimulated with phytohaemagglutinin but higher levels (>20 μg/ml for 50% reduction in cell survival) were required. After fractionation of the strawberry sample, the cytotoxicity was retained in the tannin-rich fraction and this fraction was considerably more toxic to all cells (normal or tumour cell lines or lymphocytes) than the anthocyanin-rich fraction. Established prostate (LNCaP and PC-3) and breast (MCF-7) tumour cell lines were more resistant to the strawberry extract with concentrations of 50 μg/ml required for 50% reduction in cell survival, which is similar to levels in previous studies on the antiproliferative effects of berry extracts. Although these concentrations are much greater than possible physiological levels, they are comparable to those reported in other studies. From these findings, we conclude that there is little evidence to assume that polyphenols from strawberry have a differential cytotoxic effect on tumour cells relative to comparable normal cells from the same tissue derived from the same patient.

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“…, MEYERS et al, 2003, KATSUBE et al, 2003, OLSSON et al, 2004, HAN et al, 2005, SCHIMDT et al, 2005. Caracterização das diferentes cultivares de morango") no extrato total de morango os principais compostos fenólicos são as antocianinas e os derivados de ácido elágico.…”

Section: Tabela 18 Identificação Dos Derivados De áCido Elágico E Elunclassified

Compostos bioativos de cultivares brasileiras de morango (Fragaria x ananassa Duch.): caracterização e estudo da biodisponibilidade dos derivados de ácido elágico

Pinto¹

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AGRADECIMENTOSPrimeiramente a Deus pelo presente da vida e por tantas oportunidades recebidas nessa presente existência. À força superior que sempre me ajudou nos momentos em que era chamada aos caminhos do aprendizado. A vida realmente é uma escola onde somos chamados a fazer provas do que aprendemos.Enormes agradecimentos à minha família que sempre me incentivou e me apoiou nas minhas decisões. Minha mãe, essa pessoa maravilhosa, alicerce e exemplo a ser seguido de determinação e coragem. Ao meu irmão que me descontraiu em muitos momentos em que necessitava relaxar. Ao meu pai (in memoriam) que me ensinou a retidão de caráter e a seguir os meus sonhos mesmo que em muitos momentos houvessem quedas. Aos meus tios Vitor e Lucia e primos Luiz Augusto e Carlos Augusto que tanto me auxiliaram e incentivaram nessa jornada. Gertrudes, amiga inseparável e conselheira enormes agradecimentos por ter me apoiado em muitos momentos difíceis e me fazendo acreditar que eu podia um pouco mais.Ao meu noivo Shamil Sadigov, por ter aparecido na minha vida para me apoiar em muitos momentos difíceis e pelo amor incondicional. Acredito que nada nesta vida seja fruto do acaso, e ter encontrado você foi o maior presente que poderia receber da vida. Confiança, respeito e amizade foram algumas das coisas que recebi e aprendi com você. "Я Тебя люблю"Agradeço imensamente a Prof a Dra. Maria Inés Genovese que acreditou no meu potencial e me ensinou tudo que sei. Também agradeço pela orientação, pelo seu exemplo, incentivo, dedicação, confiança, apoio e amizade. Agradeço ao Prof. Dr. Kalidas Shetty pelo período de bolsa sanduíche naUniversity of Massachusetts, Amherst, Estados Unidos. Nesse período cresci muito não só como pesquisadora mas também como pessoa, através de edificantes discussões a respeito do nosso papel nesse mundo. Nunca esquecerei do apoio que recebi quando cheguei em um país com cultura e língua totalmente diferentes. "Be good" "Be happy"(heheheheehh). Também agradeço aos meus colegas de laboratório no Department of were extraction with 80% acetone, and hydrolysis using 2N TFA at 120 C for 60 minutes. The precipitation method using ytterbium acetate was the most efficient in purifying the ellagitannins. After administration of purified ellagitannins to rats by gavage, no compound was detected in plasma or tissue samples analyzed. (PASCAL, 1996). Em alguns estudos epidemiológicos observou-se que o risco de doenças coronarianas foi reduzido devido à alta ingestão de flavonóides como as flavonas apigenina e luteolina e os flavonóis campferol, miricetina e quercetina (HERTOG et al., 1992; proliferativos, e inibição da formação de lesões pré-neoplásicas. Diversos estudos também já relataram as propriedades antioxidantes in vitro doselagitaninos e do ácido elágico (GIL et al., 2000;ANDERSON et al., 2001; MULLEN et al., 2002;SRINIVASAN et al., 2002 (MANACH et al., 2004).De forma geral, os compostos fenólicos se apresentam na forma de glicosídeos, ésteres ou polímeros e só podem ser absorvidos após hidrólise por enzimas intes...

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Inhibition of the Growth of Premalignant and Malignant Human Oral Cell Lines by Extracts and Components of Black Raspberries

Cited by 92 publications

References 38 publications

Cancer prevention with freeze-dried berries and berry components

Cancer prevention with freeze-dried berries and berry components

Strawberry polyphenols are equally cytotoxic to tumourigenic and normal human breast and prostate cell lines

Compostos bioativos de cultivares brasileiras de morango (Fragaria x ananassa Duch.): caracterização e estudo da biodisponibilidade dos derivados de ácido elágico

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