We have established a reliable method for culturing transitional cell tumors of the human urinary bladder. Out of seventy-four set up in culture, forty-eight were successfully maintained and their behavior studied using metaphase arrest and 125IUdR uptake.
Electron microscopy studies of the ultrastructure of bladder carcinoma treated by intravesical instillation of ethoglucid and mitomycin are described. Ethoglucid acts mainly by disrupting cells' intercellular bonds. Mitomycin acts on the cell nucleus. It is suggested that these differences in action form a basis for sequential therapy.
The proliferative responses of human prostatic carcinoma have been evaluated in organ culture using [125I]-iododeoxyuridine ([125I]UdR) to monitor DNA synthesis. The morphological preservation was not influenced by the addition of fetal calf serum or insulin (5 mu gm/ml), transferrin (10 mu gm/ml), and thyrotropin releasing hormone (10(-9) M) to the active medium. Testosterone (4 X 10(-7) M) stimulated [125I]UdR uptake, whereas diethylstilboestrol (4 X 10(-6) M) had no direct effect on uptake. Both estramustine phosphate (4 X 10(-6) M) and oestradiol-17 beta (4 X 10(-6) M) inhibited uptake in a similar manner. Thus while explants of human prostatic carcinoma derived from transurethrally resected specimens can be well maintained in organ culture for a few days, proliferative responses are small and difficult to measure for individual patients.
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