Inhibition of the antioxidant activity of catalase and superoxide dismutase from<i>Fusarium verticillioides</i>exposed to a<i>Jacquinia macrocarpa</i>antifungal fraction

Valenzuela-Cota, Daniel Fernando; Buitimea-Cantúa, Génesis V.; Plascencia‐Jatomea, Maribel; Cinco-Moroyoqui, Francisco Javier; Martínez-Higuera, Aarón; Rosas‐Burgos, Ema Carina

doi:10.1080/03601234.2019.1622978

Cited by 15 publications

(10 citation statements)

References 44 publications

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“…Evidence for an increased antioxidative stress response of F. pseudograminearum was up-regulation of one catalase, six GST, and four ABC transporter DEGs at both time points. Catalase breaks hydrogen peroxide into oxygen and water [ 51 ], GST can conjugate the reduced form of glutathione to xenobiotics for detoxification [ 52 ], and ABC transporter can transfer substrates across membranes to remove toxins from cells [ 53 ]. Similarly, C. gloeosporioides, Setosphaeria turcica, and B. cinerea DEGs for GSTs and/or ABC transporters were up-regulated with Bacillus stress [ 39 , 42 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Response of Fusarium pseudograminearum to Biocontrol Agent Bacillus velezensis YB-185 by Phenotypic and Transcriptome Analysis

Zhang

Zhu

Lin

et al. 2022

JoF

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The use of biological control agents (BCAs) is a promising alternative control measure for Fusarium crown rot (FCR) of wheat caused by Fusarium pseudograminearum. A bacterial strain, YB-185, was isolated from the soil of wheat plants with FCR and identified as Bacillus velezensis. YB-185 exhibited strong inhibition of F. pseudograminearum mycelial growth and conidial germination in culture. Seed treatment with YB-185 in greenhouse and field resulted in reductions in disease by 66.1% and 57.6%, respectively, along with increased grain yield. Microscopy of infected root tissues confirmed that YB-185 reduced root invasion by F. pseudograminearum. RNA-seq of F. pseudograminearum during co-cultivation with B. velezensis YB-185 revealed 5086 differentially expressed genes (DEGs) compared to the control. Down-regulated DEGs included genes for glucan synthesis, fatty acid synthesis, mechanosensitive ion channels, superoxide dismutase, peroxiredoxin, thioredoxin, and plant-cell-wall-degrading enzymes, whereas up-regulated DEGs included genes for chitin synthesis, ergosterol synthesis, glutathione S-transferase, catalase, and ABC transporters. In addition, fungal cell apoptosis increased significantly, as indicated by TUNEL staining, and the scavenging rate of 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS·+) in the fungus significantly decreased. Thus, F. pseudograminearum may be trying to maintain normal cell functions by increasing cell wall and membrane synthesis, antioxidant and anti-stress responses, detoxification of bacterial antimicrobial compounds, and transportation of damaging compounds from its cells. However, cell death and free radical accumulation still occurred, indicating that the responses were insufficient to prevent cell damage. Bacillus velezensis YB-185 is a promising BCA against FCR that acts by directly damaging F. pseudograminearum, thus reducing its ability to colonize roots and produce symptoms.

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Section: Discussionmentioning

confidence: 99%

Response of Fusarium pseudograminearum to Biocontrol Agent Bacillus velezensis YB-185 by Phenotypic and Transcriptome Analysis

Zhang

Zhu

Lin

et al. 2022

JoF

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“…It decomposes hydrogen peroxide and protects the tissue from highly reactive hydroxyl radicals. CAT is part of the antioxidant enzymes that play an indispensable role in the antioxidant protective capacity of biological systems against ROS by regulating the production of free radicals and their metabolites, so the inhibition of these enzymes can cause ROS accumulation and cellular damage (Valenzuela-Cota et al, 2019). Inhibition of this enzyme may enhance sensitivity to free radical-induced cellular damage.…”

Section: In Vivo Evaluation Reveals High Catalase Activity In Leaves ...mentioning

confidence: 99%

In vitro and in vivo antioxidant therapeutic evaluation of Dodonaea viscosa Jacq.

Khan

Rehman

Khan

et al. 2022

Preprint

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Context: Natural antioxidants are vital to promote health and treat critical diseased conditions in the modern healthcare system. Objective: This work adds to the index of natural medicines by exploring the antioxidant potential of Dodonaea viscosa Jacq. (Plant-DV). Material and methods: The aqueous extract of leaves and seeds from plant-DV in freshly prepared phosphate buffer is evaluated for antioxidant potential. In vitro antioxidant potential of the nascent and oxidatively stressed extracts was analyzed through glutathione (GSH) assay, hydrogen peroxide (H2O2) scavenging effect, glutathione-S-transferase (GST) assay, and catalase (CAT) activity. In vivo therapeutic assessment is performed in Wistar Albino rats using vitamin C as a positive control. The livers and kidneys of individual animals are probed for glutathione, glutathione-S-transferase, and catalase activities. Results: Seeds have GSH contents (59.61 microM) and leaves (32.87 microM) in the fresh aqueous extracts. The hydrogen peroxide scavenging effect of leaves is superior to seeds with 17.25% and 14.18% respectively after 30 min incubation. However, oxidatively stressed extracts with AgI and HgII show declining GSH and GST levels. The plant extracts are non-toxic in rats at 5000 mg/Kg body weight. Liver and kidneys homogenate reveal an increase in GSH, GST, and CAT levels after treatment with 150 mg/kg and 300 mg/kg body weight plant extract compared with normal saline-treated negative and vitamin C treated positive control. Discussion and Conclusion: The crude aqueous extracts of leaves and seeds of plant DV show promising antioxidant potential both in in vitro and in vivo evaluation.

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“…Additionally, a collection of different compounds found in the plant Jacquima macrocara that inhibit the growth and spore germination of Fusarium verticillioides inhibits catalase activity completely at 1.25 mg/mL of the plant extract ( Valenzuela-Cota et al, 2019 ). The repercussions of finding novel antimicrobial compounds that one of its targets is the antioxidant capacity of pathogenic fungi is worth exploring further, not only for human pathogens but also for veterinary purposes and phytopathogenic fungi.…”

Section: Future Researchmentioning

confidence: 99%

Catalases in the pathogenesis ofSporothrix schenckiiresearch

Vargas-Maya

Olmedo-Monfil

Ramírez-Prado

et al. 2022

PeerJ

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Pathogenic fungal infection success depends on the ability to escape the immune response. Most strategies for fungal infection control are focused on the inhibition of virulence factors and increasing the effectiveness of antifungal drugs. Nevertheless, little attention has been focused on their physiological resistance to the host immune system. Hints may be found in pathogenic fungi that also inhabit the soil. In nature, the saprophyte lifestyle of fungi is also associated with predators that can induce oxidative stress upon cell damage. The natural sources of nutrients for fungi are linked to cellulose degradation, which in turn generates reactive oxygen species (ROS). Overall, the antioxidant arsenal needed to thrive both in free-living and pathogenic lifestyles in fungi is fundamental for success. In this review, we present recent findings regarding catalases and oxidative stress in fungi and how these can be in close relationship with pathogenesis. Additionally, special focus is placed on catalases of Sporothrix schenckii as a pathogenic model with a dual lifestyle. It is assumed that catalase expression is activated upon exposure to H2O2, but there are reports where this is not always the case. Additionally, it may be relevant to consider the role of catalases in S. schenckii survival in the saprophytic lifestyle and why their study can assess their involvement in the survival and therefore, in the virulence phenotype of different species of Sporothrix and when each of the three catalases are required. Also, studying antioxidant mechanisms in other isolates of pathogenic and free-living fungi may be linked to the virulence phenotype and be potential therapeutic and diagnostic targets. Thus, the rationale for this review to place focus on fungal catalases and their role in pathogenesis in addition to counteracting the effect of immune system reactive oxygen species. Fungi that thrive in soil and have mammal hosts could shed light on the importance of these enzymes in the two types of lifestyles. We look forward to encouraging more research in a myriad of areas on catalase biology with a focus on basic and applied objectives and placing these enzymes as virulence determinants.

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Inhibition of the antioxidant activity of catalase and superoxide dismutase fromFusarium verticillioidesexposed to aJacquinia macrocarpaantifungal fraction

Cited by 15 publications

References 44 publications

Response of Fusarium pseudograminearum to Biocontrol Agent Bacillus velezensis YB-185 by Phenotypic and Transcriptome Analysis

Response of Fusarium pseudograminearum to Biocontrol Agent Bacillus velezensis YB-185 by Phenotypic and Transcriptome Analysis

In vitro and in vivo antioxidant therapeutic evaluation of Dodonaea viscosa Jacq.

Catalases in the pathogenesis ofSporothrix schenckiiresearch

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