In vitro and in vivo antioxidant therapeutic evaluation of Dodonaea viscosa Jacq.

Khan, Siraj; Rehman, Mujeeb Ur; Khan, Muhammad Zafar; Muhammad, Khan; Haq, İhsan Ul; Khan, M. Ijaz

doi:10.1101/2022.04.17.488588

Cited by 9 publications

(10 citation statements)

References 29 publications

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“…Many biological entities produce catalase to counteract oxidative stress by neutralizing reactive oxygen species, which are often prevalent in conditions like aging, cataracts, and cancer. [ 62,78 ] Consequently, assessing catalase levels serves as another method to gauge oxidative stress. The catalase assay outcomes are depicted in Figure 10E.…”

Section: Resultsmentioning

confidence: 99%

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3D‐QSAR pharmacophore modeling, virtual screening, molecular docking, MD simulations, in vitro and in vivo studies to identify potential anti‐hyperplasia drugs

Khan,

Akbar

et al. 2024

Biotechnology Journal

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Psoriasis is a common immune‐mediated skin condition characterized by aberrant keratinocytes and cell proliferation. The purpose of this study was to explore the FDA‐approved drugs by 3D‐QSAR pharmacophore model and evaluate their efficiency by in‐silico, in vitro, and in vivo psoriasis animal model. A 3D‐QSAR pharmacophore model was developed by utilizing HypoGen algorithm using the structural features of 48 diaryl derivatives with diverse molecular patterns. The model was validated by a test set of 27 compounds, by cost analysis method, and Fischer's randomization test. The correlation coefficient of the best model (Hypo2) was 0.9601 for the training set while it was 0.805 for the test set. The selected model was taken as a 3D query for the virtual screening of over 3000 FDA‐approved drugs. Compounds mapped with the pharmacophore model were further screened through molecular docking. The hits that showed the best docking results were screened through in silico skin toxicity approach. Top five hits were selected for the MD simulation studies. Based on MD simulations results, the best two hit molecules, that is, ebastine (Ebs) and mebeverine (Mbv) were selected for in vitro and in vivo antioxidant studies performed in mice. TNF‐α and COX pro‐inflammatory mediators, biochemical assays, histopathological analyses, and immunohistochemistry observations confirmed the anti‐inflammatory response of the selected drugs. Based on these findings, it appeared that Ebs can effectively treat psoriasis‐like skin lesions and down‐regulate inflammatory responses which was consistent with docking predictions and could potentially be employed for further research on inflammation‐related skin illnesses such as psoriasis.

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Section: Resultsmentioning

confidence: 99%

“…Coverslips were mounted after applying mountain media and the slides were air‐dried at room temperature. [ 65 ] The ImageJ software [ 66 ] was utilized in order to quantitatively analyze the expression of various markers.…”

Section: Methodsmentioning

confidence: 99%

3D‐QSAR pharmacophore modeling, virtual screening, molecular docking, MD simulations, in vitro and in vivo studies to identify potential anti‐hyperplasia drugs

Khan,

Akbar

et al. 2024

Biotechnology Journal

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“…According to the method adopted by Khan et al (2022) [ 78 ], different concentrations of OrP were added to 40 mM hydrogen peroxide solution (0.6 mL). After 10 min, the absorbance of the hydrogen peroxide at 230 nm was read against the blank solution containing the phosphate buffer without the hydrogen peroxide.…”

Section: Methodsmentioning

confidence: 99%

Hepatoprotective Effect of Opuntia robusta Fruit Biocomponents in a Rat Model of Thioacetamide-Induced Liver Fibrosis

Pulido-Hornedo

Ventura-Juárez

Guevara‐Lara

et al. 2022

Plants

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Liver fibrosis is a chronic disease associated with oxidative stress that has a great impact on the population mortality. Due to their antioxidant capacity, we evaluated the protective effect of Opuntia robusta fruit (Or) on liver fibrosis. A nutraceutical characterization of Or was performed and a model of fibrosis was induced with thioacetamide (TAA) in Wistar rats. Aminotransferases, reduced glutathione (GSH) and histopathology were evaluated. Or contained 436.5 ± 57 mg of Betacyanins equivalents/L., 793 mg of catechin equivalents (CAE)/100 g for flavonoids, 1118 mg of gallic acid equivalents (GAE)/100 g for total phenols, 141.14 mg/100 g for vitamin C and 429.9 μg/100 g for vitamin E. The antioxidant capacity of Or was: 2.27 mmol of Trolox® equivalents (TE)/L (DPPH), 62.2 ± 5.0 μmol TE/g (ABTS•+), 80.2 ± 11.7 μmol TE/g (FRAP), 247.9 ± 15.6 µmol TE/g (AAPH) and 15.0% of H2O2 elimination. An increase (p < 0.05) of aminotransferases and a decrease (p < 0.05) of hepatic GSH was observed in the TAA group compared to the control and the concomitant groups. Histopathology showed changes in the normal architecture of the liver treated with TAA compared to the concomitant treatments. Or contains bioactive components with antioxidant capacity, which can reduce fibrotic liver damage.

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“…Hence, a binary logistic regression (BLR) method was used for the analysis of farmers' perceptions of climate change and the suggested coping strategies among smallholder irrigators. Several publications such as Alemayehu and Bewket (2017), Khan et al (2020), and Ali et al (2021) have used the BLR to analyse coping strategies in farming systems. This analysis helps to understand smallholder irrigators' perception of climate change and the benefits to adapt to climate change in the schemes.…”

Section: Data Analysismentioning

confidence: 99%

Perception of climate change and coping strategies among smallholder irrigators in Zimbabwe

Mwadzingeni¹,

Mugandani²,

Mafongoya³

2023

Front. Sustain. Food Syst.

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IntroductionAcross sub-Sahara Africa, governments and international aid agencies are making huge investments in smallholder irrigation schemes to enhance food security, climate resilience and economic transformation in rain-fed farming systems. Unfortunately, a majority of the smallholder irrigation schemes have performed dismally on these fronts. Climate change is a major exacerbating factor to existing challenges resulting in the poor performance of the schemes. Hence, it is crucial to understand smallholder irrigators' perceptions of climate change and current adaptation strategies to co-design appropriate and acceptable adaptation strategies to address water stress in the schemes. This area had received less significant research attention. This study aims to determine the perception of climate change and coping strategies in smallholder schemes.MethodsA mixed-method research strategy was used to collect data from 317 irrigation scheme farmers in three schemes (Exchange, Insukamini, and Ruchanyu) in Midlands Province. A binary logistic regression (BLR) method was used for data analysis.ResultsThe results suggest that scheme farmers have noticed changes in temperature and rainfall patterns. Results obtained from the model show that climate change perception was mainly influenced by age, gender, location, irrigation experience, and plot size. Farmers perceived that climatic change has resulted in decreased irrigation water availability, thus leading to poor yields. This study also shows that the main adaptation strategies to water stress include improving soil moisture conservation, construction of small-scale reservoirs, water charging and trade, setting clear water use priorities, and adoption of climate-resilient and short-season crop varieties. Perception of high temperatures, long dry periods, late rainfall, increase in the frequency of drought, shortening of cold season, and shortening of rain season influence adaptation strategies adopted by scheme farmers.ConclusionThis study offered useful data for policymakers and irrigation developers to develop appropriate policies and programs to improve the sustainability of schemes given current and projected water stress in Zimbabwe and sub-Sahara Africa in general.

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In vitro and in vivo antioxidant therapeutic evaluation of Dodonaea viscosa Jacq.

Cited by 9 publications

References 29 publications

3D‐QSAR pharmacophore modeling, virtual screening, molecular docking, MD simulations, in vitro and in vivo studies to identify potential anti‐hyperplasia drugs

3D‐QSAR pharmacophore modeling, virtual screening, molecular docking, MD simulations, in vitro and in vivo studies to identify potential anti‐hyperplasia drugs

Hepatoprotective Effect of Opuntia robusta Fruit Biocomponents in a Rat Model of Thioacetamide-Induced Liver Fibrosis

Perception of climate change and coping strategies among smallholder irrigators in Zimbabwe

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