Inhibition of the acute-phase response in a human hepatoma cell line

Raynes, John G.; Bevan, Sarah

doi:10.1007/bf01991140

Cited by 8 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous reports have demonstrated that HepG2 and HuH7 cells are responsive to cytokines and capable of eliciting an acute phase response, such as that which occurs in vivo. 27 Indeed, cDNA microarray analysis of RNA samples obtained in this study also confirmed induction of several acute phase proteins in treated cells (Tables 2 and 3).…”

Section: Discussionsupporting

confidence: 71%

Cytokines Alter the Expression and Activity of the Multidrug Resistance Transporters in Human Hepatoma Cell Lines; Analysis Using RT‐PCR and cDNA Microarrays

Lee

Piquette‐Miller

2003

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 71%

Cytokines Alter the Expression and Activity of the Multidrug Resistance Transporters in Human Hepatoma Cell Lines; Analysis Using RT‐PCR and cDNA Microarrays

Lee

Piquette‐Miller

2003

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

“…This well-differentiated hepatoma cell line responds to a variety of pro-inflammatory cytokines and has been used for hepatitis, cytokine and post-transcriptional studies. [16][17][18][19] First, GFP reporter constructs fused to 3'UTRs containing natural GREs of the genes TGIF2, SMC5, CDK6, JUN and TNFRS1B were tested and showed enhanced mRNA decay (Fig. 4A).…”

Section: Discussionmentioning

confidence: 99%

“…It is hepatoma cell line that has been previously used for hepatitis, cytokine and post-transcriptional studies. [16][17][18][19] Transfection was performed with GFP reporter constructs with the indicated 3'UTRs using Lipofectamine 2000 reagent (Invitrogen). For RNA analysis experiments, transfections were ©2 0 1 1 L a n d e s B i o s c i e n c e .…”

mentioning

confidence: 99%

Global assessment of GU-rich regulatory content and function in the human transcriptome

et al. 2011

View full text Add to dashboard Cite

The hepatocyte growth factor regulates the synthesis of acute-phase proteins in human hepatocytes: Divergent effect on interleukin-6-stimulated genes

et al. 1996

View full text Add to dashboard Cite

patic-specific genes and antagonizes, in part, the action Our study addressed the role of the human hepatocyte of IL-6. (HEPATOLOGY 1996;23:1345-1352.) growth factor (HGF), a potent mitogen for mature rat and human hepatocytes, in the regulation of specific hepatic genes. The experimental evidence obtained in pri-The hepatocyte growth factor (HGF), a heterodimeric mary cultured human hepatocytes indicates that HGF molecule of 69 and 34 kd first isolated from rat plateregulates the synthesis of plasma proteins in a dose-re-lets, 1,2 was soon recognized to be a potent mitogen for sponse fashion. It stimulates the synthesis of the negarat hepatocytes. 3 The homologous human HGF was tive acute-phase proteins albumin, transferrin, and fisubsequently cloned and expressed as recombinant bronectin, decreases that of a 1 -antichymotrypsin (ACT) and haptoglobin, and stimulates that of a 2 -macroglobu-protein. 4 The HGF is active in primary cultured adult lin (AMG), which in man is insensitive to inflammatory rat hepatocytes 5 and, as shown recently, is also a potent mediators. HGF had no effect on C-reactive protein proliferating factor for adult human hepatocytes in cul-(CRP) synthesis. These effects differ from those elicited ture. 6 by interleukin-6 (IL-6). The effects of HGF on fibrinogenHuman HGF is thought to trigger in vivo liver regenand a 1 -antitrypsin were, however, similar to those in-eration. 7,8 It markedly increases in plasma following duced by IL-6. The effects of HGF were also observed at insults that stimulate liver regeneration (fulminant hethe messenger RNA (mRNA) level. Time-course induc-patic failure, toxic hepatitis, partial hepatectomy) [9][10][11] tion experiments showed that the effects of HGF on proand in the course of cirrhosis. 12 HGF production is detein synthesis were delayed by about 48 to 72 hours, in tected in nonparenchymal liver cells (Kupffer and sinucontrast with the 12-hour lag found after IL-6 stimulation. Although the presence of glucocorticoids was not soidal endothelial cells, 7,13,14 in fibroblasts, 15 and in absolutely necessary for HGF to affect plasma protein other distal organs such as the lung and kidney. 7,16,17 synthesis, it moderately extended the effects. In pulse-Until recently, HGF was considered essentially a mitochase experiments, it was found that the action of HGF gen for hepatocytes. Recent experimental evidence inwas not due to an alteration of the rate of secretion of the dicates, however, that HGF can act as a pleiotropic proteins. The effects of HGF on the synthesis of albumin, factor in different cells. 18,19 Thus, it could be also contransferrin, fibronectin, a 1 -antichymotrypsin, and hap-ceivable that HGF, in addition to acting as a potent toglobin could be counteracted by the simultaneous mitogen for human hepatocytes, might also influence of HGF on human hepatocytes may not simply be limitedIn this study, we investigated the effects of HGF on to its mitogenic activity, but that it also regulates he-the synthesis of plasma proteins by human hepatocytes...

show abstract

Inhibition of the acute-phase response in a human hepatoma cell line

Cited by 8 publications

References 6 publications

Cytokines Alter the Expression and Activity of the Multidrug Resistance Transporters in Human Hepatoma Cell Lines; Analysis Using RT‐PCR and cDNA Microarrays

Cytokines Alter the Expression and Activity of the Multidrug Resistance Transporters in Human Hepatoma Cell Lines; Analysis Using RT‐PCR and cDNA Microarrays

Global assessment of GU-rich regulatory content and function in the human transcriptome

The hepatocyte growth factor regulates the synthesis of acute-phase proteins in human hepatocytes: Divergent effect on interleukin-6-stimulated genes

Contact Info

Product

Resources

About