Inhibition of purified bovine liver glutathione reductase with some metal ions

Tandogan, Berivan; Ulusu, Nuriye Nuray

doi:10.3109/14756360903016512

Cited by 16 publications

(11 citation statements)

References 46 publications

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“…Glutathione reductases and glutathione peroxidases utilize the reduction potential of glutathione to form oxidized glutathione while methionine sulfoxide reductases, and peroxiredoxins couple to thioredoxin oxidation. The final reduction of oxidized glutathione or thioredoxin is catalyzed by the oxidation of NADPH by glutathione reductase or thioredoxin reductase [236,243], respectively [244,245]. …”

Section: Antioxidant Transgenic Mice That Do Not Affect Lifespan But mentioning

confidence: 99%

Mouse Models of Oxidative Stress Indicate a Role for Modulating Healthy Aging

Hamilton¹,

Walsh

Remmen³

2012

J Clin Exp Pathol

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Aging is a complex process that affects every major system at the molecular, cellular and organ levels. Although the exact cause of aging is unknown, there is significant evidence that oxidative stress plays a major role in the aging process. The basis of the oxidative stress hypothesis is that aging occurs as a result of an imbalance between oxidants and antioxidants, which leads to the accrual of damaged proteins, lipids and DNA macromolecules with age. Age-dependent increases in protein oxidation and aggregates, lipofuscin, and DNA mutations contribute to age-related pathologies. Many transgenic/knockout mouse models over expressing or deficient in key antioxidant enzymes have been generated to examine the effect of oxidative stress on aging and age-related diseases. Based on currently reported lifespan studies using mice with altered antioxidant defense, there is little evidence that oxidative stress plays a role in determining lifespan. However, mice deficient in antioxidant enzymes are often more susceptible to age-related disease while mice overexpressing antioxidant enzymes often have an increase in the amount of time spent without disease, i.e., healthspan. Thus, by understanding the mechanisms that affect healthy aging, we may discover potential therapeutic targets to extend human healthspan.

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Section: Antioxidant Transgenic Mice That Do Not Affect Lifespan But mentioning

confidence: 99%

Mouse Models of Oxidative Stress Indicate a Role for Modulating Healthy Aging

Hamilton¹,

Walsh

Remmen³

2012

J Clin Exp Pathol

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“…In normal conditions GSSG is regenerated to GSH by the action of the flavo protein glutathione reductase, which uses the reduced nicotinamide adenine dinucleotide phosphate (NADPH) as a donor of electrons. Tandogan and Ulusu [48], reported that Cd inhibits GR activity. GR inhibition is noncompetitive with respect to both, GSSG and NADPH, and significantly enhances Cd-induced mortality and hepatotoxicity.…”

Section: Sulfhydryl Group Inactivation and Oxidative Stressmentioning

confidence: 99%

Liver and Cadmium Toxicity

2013

J Drug Metab Toxicol

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“…binding to -SH residues in proteins). Relatively to the lower activity of GRed it might be in part owed to inhibition due to the toxic action of metals (Lalaouni et al 2007;Tandogan and Ulusu 2010). This lower activity suggests that fewer glutathione (GSH) is being obtained through GRed cycling of oxidised glutathione (GSSG), since GSH concentration in the cell is associated both to the efficiency of GRed activity and GSH de novo synthesis (Meister and Anderson 1983).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant response and metal accumulation in tissues of Iberian green frogs (Pelophylax perezi) inhabiting a deactivated uranium mine

et al. 2011

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Human mining activities tend often to generate greatly impacted areas which remain contaminated for long periods of time, giving rise to extreme habitats. Mining sites are usually characterized for the production of metal rich effluents with very low pH. In this work we analyzed physical and chemical parameters of water from a deactivated uranium mine pond (M) and a reference site (REF) as well as their metal content. Furthermore, we determined and compared metal accumulation in liver, kidney, bones, muscle and skin of Pelophylax perezi from REF with P. perezi from M. We also determined the enzymatic activities of glutathione-S-transferases (GSTs), catalase (CAT), glutathione reductase (Gred), and glutathione peroxidase (GPx; both selenium-dependent and selenium-independent) in liver, kidney, lung and heart. Additionally, lipoperoxidation (LPO) was also assessed in the same tissues via thiobarbituric acid reactive substances (TBARS) assay and lactate dehydrogenase (LDH) activity was determined in muscle. Our results revealed that the majority of metals were in higher concentrations in tissues of organisms from M. This trend was especially evident for U whose content reached a difference of 1350 fold between REF and M organisms. None of the organs tested for antioxidant defenses revealed LPO, nonetheless, with exception for liver, all organs from the M frogs presented increased total GPx activity and selenium-dependent GPx. However, this response was significant only for the lung, probably as a consequence of the significant inhibition of CAT upstream and to cope with the subsequent increase in H(2)O(2). Lungs were the organs displaying greater responsiveness of the anti-oxidant stress system in frogs from the uranium mine area.

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Inhibition of purified bovine liver glutathione reductase with some metal ions

Cited by 16 publications

References 46 publications

Mouse Models of Oxidative Stress Indicate a Role for Modulating Healthy Aging

Mouse Models of Oxidative Stress Indicate a Role for Modulating Healthy Aging

Liver and Cadmium Toxicity

Antioxidant response and metal accumulation in tissues of Iberian green frogs (Pelophylax perezi) inhabiting a deactivated uranium mine

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