Inhibition of pancreatic and microbial lipases by proteins

Gargouri, Youssef; Julien, Robert M.; Sugihara, Akio; Verger, Robert; Sarda, Louis

doi:10.1016/0005-2760(84)90082-1

Cited by 131 publications

(119 citation statements)

References 17 publications

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“…The lipase activity was titrimetrically measured with a pH-Stat (Metrohm, Herisau, Switzerland) under optimal conditions using olive oil emulsion as a substrate [15]. The activity was expressed as units per mL of enzymatic solution.…”

Section: Lipase Hydrolytic Activitymentioning

confidence: 99%

Enzymatic propyl gallate synthesis in solvent-free system: Optimization by response surface methodology

Bouaziz¹,

Horchani²,

Salem³

et al. 2010

Journal of Molecular Catalysis B: Enzymatic

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a b s t r a c tThe ability of a non-commercial immobilized Staphylococcus xylosus lipase to catalyze the esterification of propanol with gallic acid was investigated and the antioxidant as well as the antimicrobial activities of the ester formed were evaluated. The response surface methodology, based on a three variables Box-Behnken design (reaction temperature, enzyme amount and 1-propanol/gallic acid molar ratio), was used to optimize the experimental conditions of propylgallate synthesis. The maximum conversion yield (90% ±3.5) was obtained by using 400 IU of immobilized lipase and a propanol/gallic acid at a molar ratio of 160 at 52 • C. The obtained ester was characterized by spectroscopic methods, NMR and FTIR. The antioxidant activity of propyl gallate was evaluated and compared to the synthetic classical antioxidants, BHA and ascorbic acid, taken as references. In addition, the antimicrobial activity of the propyl gallate was tested against S. xylosus, Escherchia coli and Staphylococcus aureus using disc diffusion and macrodilution methods. Our results show that the synthesized propyl gallate ester presents a higher antioxidant and antimicrobial power than the parent gallic acid as well as the synthetic classical antioxidants.

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Section: Lipase Hydrolytic Activitymentioning

confidence: 99%

Enzymatic propyl gallate synthesis in solvent-free system: Optimization by response surface methodology

Bouaziz¹,

Horchani²,

Salem³

et al. 2010

Journal of Molecular Catalysis B: Enzymatic

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“…Melittin was a gift from Dr Gould (N.Y. State Institute for Basic Research in Mental Retardation, Staten Island, NY, USA). A proteininhibiting lipase was prepared from Soya meal as described previously [22]. Protein concentration was determined using the colorimetric method of Lowry [24] or spectrophotometrically at 280 nm using the following absorption coefficients (Al%,l cm): BSA, 6.7; P-lactoglobulin A, 9.6; ovalbumin, 7.5% ; myoglobin, 20.5 and melittin, 16.6.…”

Section: Proteinsmentioning

confidence: 99%

Human gastric lipase. The effect of amphiphiles

Gargouri

Piéroni

Lowe³

et al. 1986

Eur J Biochem

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Human gastric lipase (HGL) activity on tributyrin emulsion was detected only in the presence of amphiphiles such as bile salts, proteins (serum albumin, B-lactoglobulin or ovalbumin) or phosphatidylcholine. These findings are contrary to the strong inhibitory effect of amphiphiles observed on pure pancreatic lipase. To reveal HGL activity, amphiphiles should be added prior to HGL. This may prevent irreversible interfacial denaturation. HGL activity was found to be restricted to a triacylglycerol/water surface tension ranging from 8 dyn/cm to 13 dyn/ cm. All amphiphiles, which decrease the interfacial tension below 8 dyn/cm, act as irreversible inhibitors of HGL in the absence and in the presence of bile salts.Our results confirm that HGL is capable of hydrolysing triacylglycerol in the presence of the physiological concentration of bile salts prevailing in the upper small intestine and in the presence of alimentary proteins. These observations could explain the high dietary lipid absorption observed under pancreatic lipase deficiency.

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“…Orlistat (Ro 18-0647), a hydrogenated derivative of lipstatin derived from Streptomyces toxitricini, is a potent inhibitor of gastric, pancreatic and carboxylester lipase, 2 and has proved to be effective for the treatment of human obesity. 3 ± 5 The existence of lipase inhibitors in various foodstuffs has been researched, and the presence of these inhibitors in cereals, in wheat bran and wheat germ 6 and in soybean 7,8 has been reported. Lipase inhibitors have also been sought in natural products such as Chinese medicines and various herbs.…”

Section: Introductionmentioning

confidence: 99%

Anti-obesity effects of lipase inhibitor CT-II, an extract from edible herbs, Nomame Herba, on rats fed a high-fat diet

Yamamoto¹,

Shimura²,

Itoh³

et al. 2000

Int J Obes

108

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OBJECTIVE: To investigate the inhibitory effects of CT-II, extract of Nomame Herba, on lipase activity in vitro and on obesity in rats fed a high-fat diet in vivo. DESIGN: The assay for the inhibitory effect of CT-II on lipase activity was performed by measuring released free fatty acids after the incubation of the medium with CT-II, porcine pancreatic lipase and triolein (experiment 1). In vivo experiments, lean rats or obese rats (570 ± 718 g) were fed a high-fat diet containing 60% fat with or without CT-II for 8 weeks (experiment 2), for 14 days (experiment 3) or for 12 weeks (experiment 4), respectively. MEASUREMENT: The time course of body weight, food intake, organ weight (parametrial fat, liver, heart and kidney) and plasma parameters (triglyceride, total cholesterol, glucose, AST, ALT and insulin), fecal output of total fat and total cholesterol were measured. Hepatic histological examinations were also performed. RESULTS: CT-II inhibited the porcine lipase activity dose-dependently in vitro (experiment 1). Body and liver weight were reduced and hepatic histological examination showed an amelioration of fatty liver in CT II treated animals (experiment 2). CT-II signi®cantly inhibited body weight gain and plasma triglyceride elevation in a dose-dependent manner, without affecting food intake in lean rats fed the high-fat diet. Elevated plasma AST and ALT were also decreased (experiment 3). When obese rats fed the high-fat diet were treated with CT-II for up to 6 months, body weight was initially reduced and thereafter weight gain was signi®cantly suppressed. Total body fat was also signi®cantly reduced and signi®cant reduction of plasma AST and ALT was observed (experiment 4). CONCLUSIONS: These results demonstrated that the lipase inhibitor CT-II is effective in preventing and ameliorating obesity, fatty liver and hypertriglyceridemia in rats fed a high-fat diet.

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Inhibition of pancreatic and microbial lipases by proteins

Cited by 131 publications

References 17 publications

Enzymatic propyl gallate synthesis in solvent-free system: Optimization by response surface methodology

Enzymatic propyl gallate synthesis in solvent-free system: Optimization by response surface methodology

Human gastric lipase. The effect of amphiphiles

Anti-obesity effects of lipase inhibitor CT-II, an extract from edible herbs, Nomame Herba, on rats fed a high-fat diet

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