Anti-obesity effects of lipase inhibitor CT-II, an extract from edible herbs, Nomame Herba, on rats fed a high-fat diet

Abstract

OBJECTIVE: To investigate the inhibitory effects of CT-II, extract of Nomame Herba, on lipase activity in vitro and on obesity in rats fed a high-fat diet in vivo. DESIGN: The assay for the inhibitory effect of CT-II on lipase activity was performed by measuring released free fatty acids after the incubation of the medium with CT-II, porcine pancreatic lipase and triolein (experiment 1). In vivo experiments, lean rats or obese rats (570 ± 718 g) were fed a high-fat diet containing 60% fat with or without CT-II for 8 weeks (experiment 2), for 14 days (experiment 3) or for 12 weeks (experiment 4), respectively. MEASUREMENT: The time course of body weight, food intake, organ weight (parametrial fat, liver, heart and kidney) and plasma parameters (triglyceride, total cholesterol, glucose, AST, ALT and insulin), fecal output of total fat and total cholesterol were measured. Hepatic histological examinations were also performed. RESULTS: CT-II inhibited the porcine lipase activity dose-dependently in vitro (experiment 1). Body and liver weight were reduced and hepatic histological examination showed an amelioration of fatty liver in CT II treated animals (experiment 2). CT-II signi®cantly inhibited body weight gain and plasma triglyceride elevation in a dose-dependent manner, without affecting food intake in lean rats fed the high-fat diet. Elevated plasma AST and ALT were also decreased (experiment 3). When obese rats fed the high-fat diet were treated with CT-II for up to 6 months, body weight was initially reduced and thereafter weight gain was signi®cantly suppressed. Total body fat was also signi®cantly reduced and signi®cant reduction of plasma AST and ALT was observed (experiment 4). CONCLUSIONS: These results demonstrated that the lipase inhibitor CT-II is effective in preventing and ameliorating obesity, fatty liver and hypertriglyceridemia in rats fed a high-fat diet.

Anti-obesity Effect of <i>Dioscorea oppositifolia</i> Extract in High-Fat Diet-Induced Obese Mice and Its Chemical Characterization

“…27) Plant materials like Cassia mimosoides (ethanol extract) and Salacia reticulate (water extract), which act as pancreatic lipase inhibitors, have been reported to suppress body weight gain in animal model. 28,29) Our previous data exhibited that the DOB down-regulates porcine pancreatic lipase activity to 57% at a concentration of 10 µg/mL in vitro. 8) In the present study, high-fat diet-induced obese mice treated with DOB (100 mg/kg body weight) elevated fecal lipid, TG, and TC output to 1.3-, 1.7-, and 1.4-fold of control, respectively.…”

Anti-obesity potentials of aqueous and methanol extracts of <i>Vernonia amygdalina</i> Del. Leaves in high-fat diet fed rats

“…The observed weight loss may have been due to loss of adipose/fat tissues as internal organs were not adversely affected. Yamamoto et al (2000) had reported that edible herbs often caused a reduction in body weight via reduction in fat mass. The observed weight loss may have arisen from a variety of pathways.…”

Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction

“…A lipase inhibitor which reduces fat digestion and thus absorption, is one of widely accepted approaches in decreasing calorie intake. [1][2][3] Simple inhibition of lipid digestion and absorption is a prerequisite, but it is not sufficient for a successful long-term control of obesity. 4 Therefore, a coordinated measure to either reduce the food intake efficiency or promote energy expenditure is desirable to achieve a long-term efficacy.…”