Inhibition of on-chip PCR using PDMS–glass hybrid microfluidic chips

Crabtree, H. John; Lauzon, Jana; Morrissey, Yuen C.; Taylor, Brian J.; Liang, Tina; Johnstone, Robert W.; Stickel, Alexander J.; Manage, Dammika P.; Atrazhev, Alexey; Backhouse, C.; Pilarski, Linda M.

doi:10.1007/s10404-012-0968-9

Cited by 20 publications

(17 citation statements)

References 78 publications

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“…These results represent the first step toward 3D printed microfluidic devices for integrated analyses of nucleic acids and other molecules in which many active and passive components are incorporated in a single device. [35][36][37][38][39][40] …”

Section: Microfluidicsmentioning

confidence: 99%

3D printed microfluidic devices with integrated valves

2015

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We report the successful fabrication and testing of 3D printed microfluidic devices with integrated membrane-based valves. Fabrication is performed with a low-cost commercially available stereolithographic 3D printer. Horizontal microfluidic channels with designed rectangular cross sectional dimensions as small as 350 μm wide and 250 μm tall are printed with 100% yield, as are cylindrical vertical microfluidic channels with 350 μm designed (210 μm actual) diameters. Based on our previous work [Rogers et al., Anal. Chem. 83, 6418 (2011)], we use a custom resin formulation tailored for low non-specific protein adsorption. Valves are fabricated with a membrane consisting of a single build layer. The fluid pressure required to open a closed valve is the same as the control pressure holding the valve closed. 3D printed valves are successfully demonstrated for up to 800 actuations.

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Section: Microfluidicsmentioning

confidence: 99%

3D printed microfluidic devices with integrated valves

2015

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“…Silanization is accomplished by filling the channel with a silanizing agent and heating the filled chip for a period of time, then removing the agent and washing the chip. Alternatively, solutions such as bovine serum albumin (BSA) [ 58 ], polyvinylpyrrolidone [ 59 ], glycerol [ 60 ], polyethylene glycol 8000 [ 59 ], and Tween 20 [ 61 ] are commonly added into the PCR solution to reduce the non-specific adsorption, thereby improving the PCR efficiency.…”

Section: Surface Treatmentmentioning

confidence: 99%

The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond

Zhu

Zhang

et al. 2020

TrAC Trends in Analytical Chemistry

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Infectious diseases, such as the most recent case of coronavirus disease 2019, have brought the prospect of point-of-care (POC) diagnostic tests into the spotlight. A rapid, accurate, low-cost, and easy-to-use test in the field could stop epidemics before they develop into full-blown pandemics. Unfortunately, despite all the advances, it still does not exist. Here, we critically review the limited number of prototypes demonstrated to date that is based on a polymerase chain reaction (PCR) and has come close to fulfill this vision. We summarize the requirements for the POC-PCR tests and then go on to discuss the PCR product-detection methods, the integration of their functional components, the potential applications, and other practical issues related to the implementation of lab-on-a-chip technologies. We conclude our review with a discussion of the latest findings on nucleic acid-based diagnosis.

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“…Due to its permeable nature, uncoated PDMS might show inhibition of the amplification reaction by adsorption or absorption of components in the amplification mix, especially the enzyme. This problem can be solved by coating of the PDMS with BSA [ 173 ]. To prevent evaporation, also parylene C can be used to coat the chip [ 174 , 175 ].…”

Section: Chip Materials For Dna Analysismentioning

confidence: 99%

Microfluidic Devices for Forensic DNA Analysis: A Review

et al. 2016

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Microfluidic devices may offer various advantages for forensic DNA analysis, such as reduced risk of contamination, shorter analysis time and direct application at the crime scene. Microfluidic chip technology has already proven to be functional and effective within medical applications, such as for point-of-care use. In the forensic field, one may expect microfluidic technology to become particularly relevant for the analysis of biological traces containing human DNA. This would require a number of consecutive steps, including sample work up, DNA amplification and detection, as well as secure storage of the sample. This article provides an extensive overview of microfluidic devices for cell lysis, DNA extraction and purification, DNA amplification and detection and analysis techniques for DNA. Topics to be discussed are polymerase chain reaction (PCR) on-chip, digital PCR (dPCR), isothermal amplification on-chip, chip materials, integrated devices and commercially available techniques. A critical overview of the opportunities and challenges of the use of chips is discussed, and developments made in forensic DNA analysis over the past 10–20 years with microfluidic systems are described. Areas in which further research is needed are indicated in a future outlook.

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Inhibition of on-chip PCR using PDMS–glass hybrid microfluidic chips

Cited by 20 publications

References 78 publications

3D printed microfluidic devices with integrated valves

3D printed microfluidic devices with integrated valves

The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond

Microfluidic Devices for Forensic DNA Analysis: A Review

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