The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond

Zhu, Hanliang; Zhang, Haoqing; Ni, Sheng Huoo; Korabečná, Marie; Yobaş, Levent; Neužil, Pavel

doi:10.1016/j.trac.2020.115984

Cited by 86 publications

(71 citation statements)

References 101 publications

(127 reference statements)

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“…On the other hand, the use of pseudovirus expressing the S protein of SARS-CoV-2 instead of the virus itself is also a great opportunity to simplify and accelerate the development of assays [38] for viral proteins. The integration of technologies to smartphone mobile devices [30] , [31] , [40] , [117] , [118] , [193] provides new opportunities in different areas. In the field of medical diagnostics, they represent a paradigm shift [193] that allows the provision of mobile health care and personalized medicine.…”

Section: Others Approachesmentioning

confidence: 99%

“…within the beginning of the pandemic to August 2020. Table S1 (supplementary material) presents a classification of the published reviews based on their main purposes: (i) reviews and minireviews about the main advances carried out in the diagnostic tests in the last months, together with a critical discussion about their utility [2] , [3] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] ; (ii) information about the biosensors and the nanotechnology is presented as a useful tool to develop new strategies for the detection of the infection and also for its treatment [8] , [11] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] , [66] , [67] ; (iii) different point of view about how the artificial intelligence (AI) can be presented as a versatile tool to control the pandemic and, even more interesting, in containing the spread of the virus [20] , [68] , [69] ; (iv) current and update information about the structure and genomic of SARS-CoV-2 [70] , [71] , [72] , [73] ...…”

Section: Introductionmentioning

confidence: 99%

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Current state of diagnostic, screening and surveillance testing methods for COVID-19 from an analytical chemistry point of view

Martín

Tena

Asuero

2021

Microchemical Journal

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Since December 2019, we have been in the battlefield with a new threat to the humanity known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this review, we describe the four main methods used for diagnosis, screening and/or surveillance of SARS-CoV-2: Real-time reverse transcription polymerase chain reaction (RT-PCR); chest computed tomography (CT); and different complementary alternatives developed in order to obtain rapid results, antigen and antibody detection. All of them compare the highlighting advantages and disadvantages from an analytical point of view. The gold standard method in terms of sensitivity and specificity is the RT-PCR. The different modifications propose to make it more rapid and applicable at point of care (POC) are also presented and discussed. CT images are limited to central hospitals. However, being combined with RT-PCR is the most robust and accurate way to confirm COVID-19 infection. Antibody tests, although unable to provide reliable results on the status of the infection, are suitable for carrying out maximum screening of the population in order to know the immune capacity. More recently, antigen tests, less sensitive than RT-PCR, have been authorized to determine in a quicker way whether the patient is infected at the time of analysis and without the need of specific instruments.

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Section: Others Approachesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Current state of diagnostic, screening and surveillance testing methods for COVID-19 from an analytical chemistry point of view

Martín

Tena

Asuero

2021

Microchemical Journal

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“…For example, the COVID-19 pandemic has highlighted the importance of rapid molecular testing for pathogen detection ( 2 ). Toward that aim, recent studies have demonstrated that common molecular diagnostic tools, such as RT-PCR and DNA melting analysis, can be performed in minutes-to-seconds ( 3 , 4 ).…”

Section: Introductionmentioning

confidence: 99%

Nearest-neighbour transition-state analysis for nucleic acid kinetics

Rejali

Zuiter

et al. 2021

Nucleic Acids Research

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We used stopped-flow to monitor hypochromicity for 43 oligonucleotide duplexes to study nucleic acid kinetics and extract transition-state parameters for association and dissociation. Reactions were performed in 1.0 M NaCl (for literature comparisons) and 2.2 mM MgCl2 (PCR conditions). Dissociation kinetics depended on sequence, increased exponentially with temperature, and transition-state parameters inversely correlated to thermodynamic parameters (r = −0.99). Association had no consistent enthalpic component, varied little with temperature or sequence, and poorly correlated to thermodynamic parameters (r = 0.28). Average association rates decreased 78% in MgCl2 compared to NaCl while dissociation was relatively insensitive to ionic conditions. A nearest-neighbour kinetic model for dissociation predicted rate constants within 3-fold of literature values (n = 11). However, a nearest-neighbour model for association appeared overparameterized and inadequate for predictions. Kinetic predictions were used to simulate published high-speed (<1 min) melting analysis and extreme (<2 min) PCR experiments. Melting simulations predicted apparent melting temperatures increase on average 2.4°C when temperature ramp rates increased from 0.1 to 32°C/s, compared to 2.8°C reported in the literature. PCR simulations revealed that denaturation kinetics are dependent on the thermocycling profile. Simulations overestimated annealing efficiencies at shorter annealing times and suggested that polymerase interactions contribute to primer-template complex stability at extension temperatures.

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“…Several low cost immunoassays and molecular diagnostic kits, including Abbott's ID NOWTM, Lumex Instruments' Microchip RT-PCR and Roche's Cobas, etc., have been developed[ [65] , [66] , [67] , [68] ]. However, serological tests are used as a surveillance technique to understand better the epidemiology of the population-wide immunity environment of COVID-19[ 69 ].…”

Section: Covid-19 Virus-host Interaction Mechanismmentioning

confidence: 99%