Inhibition of NOX4 by Cissus quadrangularis extract protects from Type 2 diabetes induced-steatohepatitis

Syed, Anees A.; Reza, Mohammad Irshad; Husain, Athar; Singh, Punj Lata; Gayen, Jiaur R.

doi:10.1016/j.phyplu.2021.100021

Cited by 15 publications

(6 citation statements)

References 25 publications

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“…Findings have shown that ROS scavenging is also essential for treating HTN and that ACEI, such as antioxidant phenols, is a preferred antihypertensive agent. Medicinal plants as well as phytopharmaceuticals have been used for the treatment of severe diseases and have least adverse effects [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Lagerstroemia speciosa Ameliorated Blood Pressure in LNAME Induced Hypertension in Experimental Rats through NO/cGMP and Oxidative Stress Modulation

Aleissa

Al-Zharani

Alneghery

et al. 2022

BioMed Research International

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Cardiovascular disease is the primary reason for chronic heart diseases and mortality worldwide. Hypertension (HTN) is the utmost dominant risk factor for the evolution of several diseases. Herbal medicines, traditional medicinal herbs, and their extracts are widely utilized to treat and monitor HTN. Herbal components have been shown to help relax arteries and lower oxidative stress. The current study assesses the probable role of herbal plant extract Lagerstroemia speciosa (LS) in the LNAME induced HTN in rats. LNAME (50 mg/100 mL) in drinkable water was given to rats for five weeks. There was a significant upsurge in LNAME-treated hypertensive rats’ blood pressure (BP). On treatment with LS, it ameliorates blood pressure. Further, LS also improved body weight, reduced heart weight, and heart hypertrophy. The NO/cGMP concentration was lowered along with a substantial upsurge in the level of glutathione and a decline in MDA level. The LS extract also reduced the inflammatory cytokine markers in the systemic circulation. In conclusion, thus, the extract of LS treatment can efficiently alleviate the BP, oxidative stress markers, and inflammation and improve NO/cGMP concentration in LNAME induced HTN in rats.

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Section: Introductionmentioning

confidence: 99%

Lagerstroemia speciosa Ameliorated Blood Pressure in LNAME Induced Hypertension in Experimental Rats through NO/cGMP and Oxidative Stress Modulation

Aleissa

Al-Zharani

Alneghery

et al. 2022

BioMed Research International

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“…Using 3T3-L1 cells, C. quadrangularis demonstrated that proliferation is reduced as the concentration rises. This demonstrates that the extract of C. quadrangularis may contain strong bioactive substances that inhibit hyperglycaemic activity [ 26 ]. The WSSP extract's bioactive components, including anthocyanins and alkaloids, are thought to be responsible for the substance's antidiabetic effects [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Analyzing the Antihyperglycemic Effect of Cissus quadrangularis and Bacopa monnieri on 3T3-L1 Cell Lines

IGK

et al. 2024

Cureus

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Background Cissus quadrangularis is a perennial shrub of the grape family. Other names for it include devil's backbone, veld grape, and pirandai (Tamil). Bacopa monnieri , a perennial plant, is native to wetlands in eastern and southern India. The 3T3-L1 cell line, which was created from 3T3 cells, was used in the scientific study. The current study's purpose is to evaluate the antihyperglycemic benefits of B. monnieri and C. quadrangularis , which will be added to the current arsenal of efficient herbal hypoglycemic medications. Aim To analyze and compare the anti-hyperglycaemic effects of the two plant extracts, C. quadrangularis and B. monnieri using a 3T3-L1 cell line. Materials and methods C. quadrangularis seeds were gathered, and extraction was conducted. The B. monnieri plant was harvested, and a rotary evaporator was used to extract the flower. Adipocyte cells were obtained from NCCS, Pune. A CO 2 incubator was used to incubate the cells. The MTT assay and gene expression analysis were done on the cell line samples. Results The antihyperglycemic effects of C. quadrangularis IRS mRNA levels of 0.7 and AKT mRNA levels of 0.7 are compared to B. monnieri IRS1 mRNA levels of 0.6 and AKT mRNA levels of 0.6 to build better diabetic treatments. The antihyperglycemic benefits of C. quadrangularis levels of IRS mRNA and AKT mRNA are compared to the influence of B. monnieri IRS1 mRNA and AKT mRNA on the development of better diabetic drugs. Conclusion Comparing the effects of C. quadrangularis and B. monnieri on the 3T3 cell line by gene expression of IRS mRNA and AKT mRNA suggests that the particular AKT downregulation shows that insulin suppresses gluconeogenesis and C. quadrangularis inhibits hyperglycemia in 3T3-L1 cells, while research on in vitro rats suggests that B. monnieri may minimize the signs and symptoms of diabetes via enhancing IRS1/AKT signaling.

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“…The stem of Cissus quadrangularis was dried and powdered, then extracted with 95% ethanol by the percolation method. The resulting extract was filtered, concentrated by rotavapor at 40 °C, and yielded 200.0 g. The stem bark extract was then characterized, and quantified using LC-MS/MS analysis as described in a previously published paper [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

Simultaneous Estimation of Quercetin and trans-Resveratrol in Cissus quadrangularis Extract in Rat Serum Using Validated LC-MS/MS Method: Application to Pharmacokinetic and Stability Studies

et al. 2023

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Cissus quadrangularis is a nutrient-rich plant with a history of use in traditional medicine. It boasts a diverse range of polyphenols, including quercetin, resveratrol, β-sitosterol, myricetin, and other compounds. We developed and validated a sensitive LC-MS/MS method to quantify quercetin and t-res biomarkers in rat serum and applied this method to pharmacokinetic and stability studies. The mass spectrometer was set to negative ionization mode for the quantification of quercetin and t-res. Phenomenex Luna (C18(2), 100 A, 75 × 4.6 mm, 3 µ) column was utilized to separate the analytes using an isocratic mobile phase consisting of methanol and 0.1% formic acid in water (82:18). Validation of the method was performed using various parameters, including linearity, specificity, accuracy, stability, intra-day, inter-day precision, and the matrix effect. There was no observed significant endogenous interference from the blank serum. The analysis was completed within 5.0 min for each run, and the lower limit of quantification was 5 ng/mL. The calibration curves showed a linear range with a high correlation coefficient (r2 > 0.99). The precision for intra- and inter-day assays showed relative standard deviations from 3.32% to 8.86% and 4.35% to 9.61%, respectively. The analytes in rat serum were stable during bench-top, freeze-thaw, and autosampler (−4 °C) stability studies. After oral administration, the analytes showed rapid absorption but underwent metabolism in rat liver microsomes despite being stable in simulated gastric and intestinal fluids. Intragastric administration resulted in higher absorption of quercetin and t-res, with greater Cmax, shorter half-life, and improved elimination. No prior research has been conducted on the oral pharmacokinetics and stability of anti-diabetic compounds in the Ethanolic extract of Cissus quadrangularis EECQ, making this the first report. Our findings can provide the knowledge of EECQ’s bioanalysis and pharmacokinetic properties which is useful for future clinical trials.

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Inhibition of NOX4 by Cissus quadrangularis extract protects from Type 2 diabetes induced-steatohepatitis

Cited by 15 publications

References 25 publications

Lagerstroemia speciosa Ameliorated Blood Pressure in LNAME Induced Hypertension in Experimental Rats through NO/cGMP and Oxidative Stress Modulation

Lagerstroemia speciosa Ameliorated Blood Pressure in LNAME Induced Hypertension in Experimental Rats through NO/cGMP and Oxidative Stress Modulation

Analyzing the Antihyperglycemic Effect of Cissus quadrangularis and Bacopa monnieri on 3T3-L1 Cell Lines

Simultaneous Estimation of Quercetin and trans-Resveratrol in Cissus quadrangularis Extract in Rat Serum Using Validated LC-MS/MS Method: Application to Pharmacokinetic and Stability Studies

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