Inhibition of nitric oxide and lipid accumulation by Sargassum sp. seaweeds and their antioxidant properties

Abstract

Recently, the use of seaweed has attracted significant attention and has resulted in several seaweed-based studies for developing health-promoting foods. Sargassum sp. has been consumed as a part of the diet for centuries, especially in Asian countries, and has been reported to exhibit diverse biological effects, such as antioxidant, anti-obesity, and anti-inflammation effects. The present study involved the evaluation of the total phenolics content and in vitro antioxidant activities of seaweed using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assays. Moreover, the inhibitory effect of lipid accumulation during the production of 3T3-L1 adipocytes and nitric oxide (NO) in RAW264.7 macrophages were investigated. The results revealed that Sargassum siliquastrum contained the highest total phenolics content (36.67 mg GAE/g of extract) and exhibited the highest antioxidant activity among the 19 Sargassum sp. seaweeds. In terms of the anti-adipogenic activity, the Sargassum nipponium and Sargassum siliquastrum extracts significantly inhibited lipid accumulation (up to 63%) compared to that of the control in 3T3-L1 cells. Additionally, the Sargassum sagamianum extract inhibited NO production (up to 85%) in lipopolysaccharide-induced RAW264.7 cells. Our results provide evidence that Sargassum sp. could be useful for the development of healthy functional foods.

Comparative Analysis of Biological Activities and Phenolic Content between Fresh and Steamed Sargassum fusiforme in Different Extraction Solvents

“…The results of this study collectively suggest that the phenolic contents in SF extracts are mod- A positive correlation (r = 0.586-0.898) was found between the total polyphenol content and the DPPH and ABTS radical scavenging activities and antioxidant enzyme (SOD, CAT, and APX) activities for SF and SSF, with a significantly high positive correlation (r = 0.898, p = 0.015) between the total polyphenol content and the APX activity (Figure 4b). You et al [87] reported a high positive correlation between the phenolic content and the DPPH and ABTS radical scavenging activities based on the increase in these activities with an increase in the phenolic compound contents in the extracts of 19 Sargassum species. Similarly, the SF and SSF extracts in the present study showed a positive correlation between the total polyphenol content and the ABTS radical scavenging activity and antioxidant enzyme activities, suggesting a close association between the total polyphenol content and the antioxidant activity in SF and SSF.…”

Antioxidant and Anti-Inflammatory Activities of Sargassum macrocarpum Extracts

“…Fucoidan is a representative active ingredient of brown algae that has anticancer and anti-inflammatory activities [ 13 , 14 ]. Nineteen Sargassum species can inhibit nitric oxide and lipid accumulation, and they have an antioxidant capacity [ 15 ]. S. serratifolium extract reduces neuroinflammation in mice [ 16 ].…”