Inhibition of NF-&amp;kappa;B activation improves insulin resistance of L6 cells

Xie, Fang; Zhu, Jian; Hou, Baolian; Wang, Yuan; Meng, Fanzhu; Ren, Zhixing; Ren, Shengxiang

doi:10.1507/endocrj.ej17-0012

Cited by 12 publications

(10 citation statements)

References 17 publications

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“…IR is often associated with the activation of MAPKs signaling (Talbot, Wheeler‐Jones, & Cleasby, 2014; Xie et al, 2017). Activated MAPKs signaling, in turn, promotes the pathological variations in diabetes.…”

Section: Discussionmentioning

confidence: 99%

Retracted : Salidroside from Rhodiola wallichiana var. cholaensis reverses insulin resistance and stimulates the GLP‐1 secretion by alleviating ROS‐mediated activation of MAPKs signaling pathway and mitigating apoptosis

Yan

Nian

et al. 2020

J. Food Biochem.

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The present study was aimed to investigate the mechanisms of salidroside (SAL) from Rhodiola wallichiana var. cholaensis on hypoglycemic and oxidative stress responses. The palmitate (PA)-induced GLUTag cells model and the glucosamine-induced insulin resistance model in HepG2 cells were built. SAL led to the up-regulation of the serum glucagon-like peptide 1 (GLP-1) level by facilitating the SCFAs production, the promotion of GLP-1 synthesis by improving p38 MAPK phosphorylation and regulating insulin resistance. Moreover, the production of reactive oxygen species (ROS) and the expression of MAPKs were down-regulated. Furthermore, SAL was found to be able to inhibit PA-induced apoptosis that down-regulates cleaved caspase-3 and Bax expressions, while up-regulating Bcl-2 expression and up-regulates the Bcl-2/ Bax ratio in glucosamine induced insulin resistance model. Besides, SAL can also upregulate the mTOR/p70S6k signaling pathway in the PA-induced GLUTag cells model. Our data demonstrated that SAL could reverse insulin resistance and stimulates the GLP-1 secretion by alleviating ROS-mediated activation of MAPKs signaling pathway and mitigating apoptosis. Practical applications Our data showed that SAL could increase the GLP-1 level by stimulating the SCFAs production and p38 phosphorylation and facilitate the IR and GLP-1 synthesis by alleviating ROS-mediated activation of MAPKs signaling pathway and mitigating apoptosis. Furthermore, the SAL has also stimulated the mTOR/p70S6k signaling pathway in PA-induced GLUTag cells model. The results provided a possibility to employ SAL for diabetes treatment.

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Section: Discussionmentioning

confidence: 99%

Retracted : Salidroside from Rhodiola wallichiana var. cholaensis reverses insulin resistance and stimulates the GLP‐1 secretion by alleviating ROS‐mediated activation of MAPKs signaling pathway and mitigating apoptosis

Yan

Nian

et al. 2020

J. Food Biochem.

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“…Oxidative stress is associated with instigating insulin resistance by up-regulating the production of pro-inflammatory cytokines, such as CRP and TNF-α, which induce damage to proteins in the insulin cascade (Styskal et al, 2012; Xie et al, 2017). Previous evidence has shown that DRT in rats with diabetes (Talebi-Garakani and Safarzade, 2013), middle-aged (Jung et al, 2015) and obesity induced by a hyperlipidic diet, reduced pro-inflammatory markers, and was associated with improved insulin sensitivity in skeletal muscle (Panveloski-Costa et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, the observed data from the DRT group corroborates with the studies mentioned above, since it induced a decrease in the expression of pro-inflammatory cytokines (CRP and TNF-α), increased anti-inflammatory (IL-4 and IL-10), and improved the inflammatory balance (e.g., increased the IL-4/TNF-α and IL-10/TNF-α ratios). We demonstrated that the DRT benefits the metabolism of glucose in the myocytes by mechanisms involving the reduction of inflammation, as pro-inflammatory cytokines such as CRP and TNF-α promote the worsening of glucose uptake through peripheral resistance in insulin action (D’Alessandris et al, 2007; Xie et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Oxidative stress, inflammation, and nitric oxide (NO) bioavailability are involved in glucose uptake by the muscle (Etgen et al, 1997; Xie et al, 2017). NO has an important role in glucose uptake signaling, wherein the decrease in NO bioavailability is associated with insulin resistance in myocytes (Etgen et al, 1997; Carvalho-Filho et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Dynamic, Not Isometric Resistance Training Improves Muscle Inflammation, Oxidative Stress and Hypertrophy in Rats

et al. 2019

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This study aimed to compare the effects of dynamic (DRT) and isometric (IRT) resistance training on blood glucose, muscle redox capacity, inflammatory state, and muscle strength and hypertrophy. Fifteen 12-week-old male Wistar rats were randomly allocated into three groups: control group (CTL), DRT, and IRT, n = 5 animals per group. The animals were submitted to a maximal weight carried (MWC; every 15 days) and maximum isometric resistance (MIR; pre- and post-training) tests. Both training protocols were performed five times a week during 12 weeks, consisting of one set of eight uninterrupted climbs for 1 min with a 30% overload of MWC. The animals in the IRT group remained under isometry for 1 min. The DRT group experienced greater MWC from pre- to post-training compared to the CTL and IRT groups (p < 0.0001). The DRT and IRT groups displayed similar gains in MIR (p = 0.3658). The DRT group exhibited improved glycemic homeostasis (p = 0.0111), redox (p < 0.0001), and inflammatory (p < 0.0001) balance as compared with CTL and IRT groups. In addition, the improved glycemic profile was associated with an increase in muscle strength and hypertrophy, improvement in redox balance and inflammation status. We conclude that DRT was more effective than IRT on increasing cross-sectional area, but not muscle strength, in parallel to improved blood glucose, inflammatory status, and redox balance.

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“…AMP-activated protein kinase (AMPK) is a cellular nutrient sensor that affects cellular energy homeostasis. 4,5 Once activated, AMPK plays a different role in cells by turning off energy-utilizing pathways and turning on energy-generating pathways. 6 Studies have shown that AMPK increases insulin sensitivity in certain cells and improves metabolic milieu that may lead to reducing the risk of T2DM.…”

Section: Introductionmentioning

confidence: 99%

Exendin-4 improves glucose metabolism in insulin-resistant cells by upregulating the phosphorylative AMPK

Li¹,

Gong²,

Qu³

et al. 2019

JAH

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Glucagon-like peptide-1 (GLP-1) is a peptide with multiple functions in regulating blood glucose with the mechnism still not throughly understand. AMP-activated protein kinase (AMPK) plays an important role in glucose and energy homeostasis, especially in type 2 diabetes mellitus (T2DM) pathophysiology. This study explores GLP-1 analogue exendin-4 (Ex-4) regulating glucose balance by activating AMPK and regulation gluconeogenesis in insulin-resistant cell model (IR/ HepG2). IR/HepG2 cells were treated with Ex-4, AMPK activator or Ex-4 pretreated with AMPK inhibitor Compound C. The change of cell morphology, glucose consumption and lipid content in IR/HepG2 cells were examined. The expression of AMPK and p-AMPK in IR/HepG2 treated by Ex-4 were determined by Western blot and immunochemisthistology (IHC) assay. Key enzymes of glucose metabolism, phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G-6-Pase) in IR/HepG2 cells treated by Ex-4 were detected by Western blot, immunohistochemistry and qRT-PCR. The results showed that Ex-4 treatment increased the expression of p-AMPK and reduced the expression of G-6-Pase and PEPCK in IR/HepG2 cells. Pretreatment with AMPK inhibitor Compound C counteracts the effect of Ex-4. These findings suggest that Ex-4 fulfilled it glucose regulation effect by phosphorylation of AMPK and decreased the expression of enzymes involving gluconeogenesis.

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Inhibition of NF-κB activation improves insulin resistance of L6 cells

Cited by 12 publications

References 17 publications

Retracted : Salidroside from Rhodiola wallichiana var. cholaensis reverses insulin resistance and stimulates the GLP‐1 secretion by alleviating ROS‐mediated activation of MAPKs signaling pathway and mitigating apoptosis

Retracted : Salidroside from Rhodiola wallichiana var. cholaensis reverses insulin resistance and stimulates the GLP‐1 secretion by alleviating ROS‐mediated activation of MAPKs signaling pathway and mitigating apoptosis

Dynamic, Not Isometric Resistance Training Improves Muscle Inflammation, Oxidative Stress and Hypertrophy in Rats

Exendin-4 improves glucose metabolism in insulin-resistant cells by upregulating the phosphorylative AMPK

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