Inhibition of Mycobacterium abscessus, M. chelonae, and M. fortuitum biofilms by Methylobacterium sp

García-Coca, Marta; Rodríguez-Sevilla, Graciela; Pérez-Domingo, Andrea; Aguilera-Correa, John Jairo; Esteban, Jaime; Muñoz-Egea, María-Carmen

doi:10.1038/s41429-019-0232-6

Cited by 12 publications

(16 citation statements)

References 31 publications

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“…Despite of its clinical importance for being directly related to this concrete outbreak, the antibacterial contact effect of the extract should be tested in other M. chimaera strains, a cocktail of strains or even other Mycobacterium species. Moreover, this Methylobacterium extract has demonstrated to have diverse effects in different rapidly growing non-tuberculous mycobacteria [33,62]. Therefore, this study should be taken as exploratory in nature.…”

Section: Discussionmentioning

confidence: 99%

“…Before each experiment, 0.5 mL of a 2.00 ± 0.02 McFarland suspension of M. chimaera ECMO made in 0.9% saline was inoculated in a Bact/Alert MP bottle (Biomérieux, Îlle de France, France) supplemented with 0.5 mL of antibiotic supplement following manufacturer instructions. The suspension was then incubated for at least 120 h at 37 • C and 5% CO 2 atmosphere [33].…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

“…Following the methodology previously described by García-Coca et al [33], a 4-McFarland suspension of Methylobacterium sp. CECT 7180 from Spanish Type Culture Collection (Colección Española de Cultivos Tipo, CECT) was made in phosphate buffer saline (PBS) (Biomérieux, Îlle de France, France).…”

Section: Methylobacterium Sp Cect 7180 Extract Elaborationmentioning

confidence: 99%

“…For the study of the adherence of M. chimaera ECMO, the protocol previously described by García-Coca et al [33], with some modifications, was employed as follow. Bact/Alert MP flasks inoculated for 120 h were centrifuged at 1160× g for 10 min (Thermo Scientific™ Sorvall™ ST 16 Centrifuge) and washed three times with PBS.…”

Section: Chimaera Ecmo Adherence Studymentioning

confidence: 99%

“…For the study of the formation of M. chimaera ECMO biofilm, the protocol described by García-Coca et al [33], with some modifications, was employed as follow. After performing the adherence protocol described above on each condition, the supernatant was removed, and the wells were washed once with PBS before adding 5 mL of Middlebrook 7H9 broth (BD, New Jersey, USA) in each well.…”

Section: Inhibition Of M Chimaera Ecmo Biofilm Formationmentioning

confidence: 99%

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Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System

et al. 2020

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Mycobacterium chimaera is an opportunistic slowly growing non-tuberculous mycobacteriumof increasing importance due to the outbreak of cases associated with contaminated 3T heater-cooler device (HCD) extracorporeal membrane oxygenator (ECMO). The aim of this study was to evaluate the effect of pre-treating a surface with a Methylobacterium sp. CECT 7180 extract to inhibit the M. chimaera ECMO biofilm as well as of the treatment after different dehydration times. Surface adherence, biofilm formation and treatment effect were evaluated by estimating colony-forming units (CFU) per square centimeter and characterizing the amount of covered surface area, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. We found that exposing a surface to the Methylobacterium sp. CECT 7180 extract inhibited M. chimaera ECMO biofilm development. This effect could be result of the effect of Methylobacterium proteins, such as DNaK, trigger factor, and xanthine oxidase. In conclusion, exposing a surface to the Methylobacteriumsp. extract inhibits M. chimaera ECMO biofilm development. Furthermore, this extract could be used as a pre-treatment prior to disinfection protocols for equipment contaminated with mycobacteria after dehydration for at least 96 h.

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Section: Discussionmentioning

confidence: 99%

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

Section: Methylobacterium Sp Cect 7180 Extract Elaborationmentioning

confidence: 99%

Section: Chimaera Ecmo Adherence Studymentioning

confidence: 99%

Section: Inhibition Of M Chimaera Ecmo Biofilm Formationmentioning

confidence: 99%

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