Inhibition of Mitogen-Induced Lymphocyte Proliferative Responses by Quinine

Thong, Y.H.; Fèrrante, Antonio

doi:10.4269/ajtmh.1978.27.354

Cited by 58 publications

(65 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, lipid-soluble tetracyclines have been reported to accumulate in leucocytes [9,41,42]. In agreement with the latter are the in vitro sludies that demonstrated that lipidsoluble tetracyclines are more potent inhibitors of mitogenindueed prohferation than non-lipid-soluble ones [13][14][15][16].…”

Section: Discussionsupporting

confidence: 69%

See 1 more Smart Citation

The influence of tetracyclines on T cell activation

Kloppenburg

Verweij

Miltenburg

et al. 1995

Clinical and Experimental Immunology

106

View full text Add to dashboard Cite

SUMMARYMinocycHne has been shown to have an anti-inflammatory effect in patients with rhetimatoid arthritis (RA). Since there is evidence thai RA is a T cell-mediated disease, we investigated Ihe efl'ect of minocycline on human T cell clones derived from the synovium of an RA patient. The T cells, when activated via the T cell receptor {TCR)/CD3 complex, were suppressed functioniiUy by minocyciine, resulting in a dose-dependent inhibition of T eell proliferation and reduction in production of lL-2. inlerferon-gamma (IFN-7) and tumour necrosis faetor-alpha (TNF-Q). Besides an inhibition of IL-2 production, mitiocycline exerted its effect on T cell proliferation by induction of a decreased IL-2 responsiveness. We showed that the ehelating capacity of minocycline plays a crucial role in the inhibitory effect on T eell function, since the inhibitory elTecl on T cell proliferation could be annulled hy addition of exogenous Ca^^. However, minocycline did nol markedly influence the typical TCR/CD3-induced intracellular Ca^* mobilization. Taken together. Ihe results elearly indicate that minocycline has immunomodulating effects on human T cells.

show abstract

Section: Discussionsupporting

confidence: 69%

“…the rejection of transplants [8][9][10][11] and levels of serum immunoglobulin [9,12]. Furthermore, in vitro tetracyclines inhibited the proliferative response of human peripheral blood mononuclear cells (PBMC) to mitogens [13][14][15][16].…”

Section: Introdljctionmentioning

confidence: 99%

The influence of tetracyclines on T cell activation

Kloppenburg

Verweij

Miltenburg

et al. 1995

Clinical and Experimental Immunology

106

View full text Add to dashboard Cite

show abstract

“…Since the patients' lymphocytes consistently showed little transformation by the drugs at concentrations higher than 39 ,uM, the contributioni of reported inhibitory effects of the drugs on the lymphocytes (18,19) When platelets were fractionated and either the 100,000 g membrane pellet or supernate were used instead of platelets in the presence of drugs in cultured lymphocytes from patients, transforming activity was seen to reside in the membrane fraction (Table III). On the basis of protein content the membranes were more effective than intact platelets.…”

Section: Resultsmentioning

confidence: 99%

Study of the factors that cause specific transformation in cultures of lymphocytes from patients with quinine- and quinidine-induced immune thrombocytopenia.

Hosseinzadeh¹,

Firkin²,

Pfueller³

1980

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Quinine-or quinidine-induced thrombocytopenic purpura is caused by synthesis of an immunoglobulin (Ig)G antibody, which causes platelet damage in the presence of the offending drug. The nature of the antigenic stimulus has been examined by measuring incorporation of [3H]thymidine into DNA during lymphocyte transformation to blast cells in the presence of the drug. Although patients' lymphocytes responded normally to the nonspecific mitogen, phytohemagglutinin P, they did not respond to either drug or platelets alone. However, significant transformation occurred when patients' lymphocytes were cultured for 7 d with homologous or autologous platelets in the presence of therapeutic concentrations of the drugs (0.39-39 ,M). Platelet membranes were more active than intact platelets on the basis of protein content, whereas platelets from a patient with Bernard-Soulier syndrome were inactive. Washed platelets pretreated with the drugs were inactive when cultured with lymphocytes in the absence of the drugs, whereas platelets pretreated similarly in plasma caused transformation. Control lymphocytes from 20 normal patients and 6 patients with nondrug-induced thrombocytopenia were not transformed by drugs and platelets in the presence of normal serum or serum containing drug-dependent antibody, showing that the observed response was specific for presensitized lymphocytes. Thus lymphocytes of patients with drug-induced thrombocytopenia are transformed by an antigen that forms after interaction ofplasma, specific platelet membrane components and the drug.A preliminary report of this work appeared as an abstract in 1979. Thromb. Haemostasis. 42: 1.

show abstract

“…Certain tetracycline drugs (such as doxycycine and minocycine), but not others (such as tetracycline and oxytetracycline), suppress the growth of mitogenstimulated human lymphocytes when present in the culture fluid at concentrations comparable to peak blood levels sometimes achieved during treatment of infections (Banck & Forsgren, 1979;Thong & Ferranti, 1979). Finch (1980) has argued that it is unlikely that any of the tetracycline drugs will have an appreciable adverse effect on the immune response during the treatment of an acute infection in a previously healthy person since the duration of treatment with the antibiotic is short relative to the timecourse of the immunological reaction, but he has emphasized that the immunosuppressive effects of antimicrobial agents may be clinically relevant in long-term chemotherapy for chronic infections.…”

Section: Methodsmentioning

confidence: 99%

Some tetracycline drugs suppress mitogen‐stimulated lymphocyte growth but others do not.

Potts¹,

MacConnachie²,

Brown³

et al. 1983

Brit J Clinical Pharma

View full text Add to dashboard Cite

1 Eight tetracycline drugs were tested for inhibitory effect on phytohaemagglutinin (PHA) stimulated growth (measured by [3H]-TdR uptake) of peripheral blood mononuclear cells: at least 26 normal subjects were tested with each drug. 2 Doxycycline reduced DNA synthesis appreciably at concentrations within and just above the therapeutic range of blood levels: it was the most potent inhibitor, but demeclocycine, methacycline and minocycline had similar, if less potent, effects. Tetracycline, oxytetracycine, chlortetracycline and clomocycline did not inhibit DNA synthesis of 3-day cultures even at concentrations five to ten times greater than the therapeutic blood level. 3 Volume spectroscopy measurements showed that none of the eight tetracycline drugs interfered with the recruitment of cells into GI-phase growth of the first cell cycle after PHA stimulation.

show abstract

Inhibition of Mitogen-Induced Lymphocyte Proliferative Responses by Quinine

Cited by 58 publications

References 0 publications

The influence of tetracyclines on T cell activation

The influence of tetracyclines on T cell activation

Study of the factors that cause specific transformation in cultures of lymphocytes from patients with quinine- and quinidine-induced immune thrombocytopenia.

Some tetracycline drugs suppress mitogen‐stimulated lymphocyte growth but others do not.

Contact Info

Product

Resources

About