Inhibition of metastasis and growth of breast cancer by pH-sensitive poly (β-amino ester) nanoparticles co-delivering two siRNA and paclitaxel

Tang, Shan; Yin, Qi; Su, Jinghan; Sun, Hongwei; Meng, Qingshu; Chen, Yi; Chen, Lingli; Huang, Yongzhuo; Gu, Wanyi; Xu, Ming‐Hua; Yu, Haijun; Zhang, Zhiwen; Li, Yaping

doi:10.1016/j.biomaterials.2015.01.049

Cited by 133 publications

(63 citation statements)

References 53 publications

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“…This result suggests that H6R6-NP had a significant inhibitory effect on the tumor growth of 4T1 cells, and this finding was consistent with that found in previous literature. 41,42 This phenomenon ascribes that H6R6-CS copolymer as a non-viral vector can be used to encapsulate siRNA effectively to form a stable NP, which protects siRNA from being degraded by nuclease in tumor tissues. Moreover, H6R6-NP possessed higher membranepenetrating capacity and better endosomal escape effect.…”

Section: Results Of Animal Experimentsmentioning

confidence: 99%

siRNA-loaded poly(histidine-arginine)<sub>6</sub>-modified chitosan nanoparticle with enhanced cell-penetrating and endosomal escape capacities for suppressing breast tumor metastasis

Park¹,

Huang²,

Kang³

et al. 2017

IJN

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An ideal carrier that delivers small interfering RNA (siRNA) should be designed based on two criteria: cellular-mediated internalization and endosomal escape. Poly(histidine-arginine) 6 (H6R6) peptide was introduced into chitosan (CS) to create a new CS derivative for siRNA delivery, 6-polyarginine (R6) as cell-penetrating peptides facilitated nanoparticle cellular internalization has been proved in our previous research, and 6-polyhistidine (H6) mediated the nanoparticle endosome escape resulted in the siRNA rapid releasing into tumor cytoplasm. H6R6-modified CS nanoparticles showed higher transfection efficiency and better endosomal escape capacity compared to ungroomed CS nanoparticle in vitro. Noticeably, H6R6-modified CS nanoparticles effectively inhibited tumor cell growth and metastases in vivo and significantly improved survival ratio. Therefore, we concluded that H6R6-modified CS copolymer can act as an ideal carrier for siRNA delivery and as a promising candidate in breast cancer therapy.

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Section: Results Of Animal Experimentsmentioning

confidence: 99%

siRNA-loaded poly(histidine-arginine)<sub>6</sub>-modified chitosan nanoparticle with enhanced cell-penetrating and endosomal escape capacities for suppressing breast tumor metastasis

Park¹,

Huang²,

Kang³

et al. 2017

IJN

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“…As a first line drug for breast cancer, paclitaxel presents a good effect (Carbognin et al, 2015;Tang et al, 2015) (Carbognin et al, 2015;Tang et al, 2015). However, in clinical use, a single paclitaxel treatment often cannot restrain the breast cancer progress.…”

Section: Introductionmentioning

confidence: 99%

Effect of lipoic acid combined with paclitaxel on breast cancer cells

Ren

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Breast cancer is the most common gynecologic tumor globally that threatens women's health. Lipoic acid is a type of antioxidant that can alleviate oxidative stress damage. Studies showed that lipoic acid could inhibit the proliferation of tumor cells in cervical cancer and colon cancer. This paper intends to explore the combined effect of lipoic acid and paclitaxel on breast cancer cells. Breast cancer MCF-7 cells were divided into four groups: control group, lipoic acid group, paclitaxel group, and a combination group. MTT was applied to detect the drugs' influence on breast cancer cell proliferation. A colony formation test was used to determine the effects on breast cancer cell clone formation rate. Western blot was performed to detect the effects on nuclear factor (NF)-κB. Lipoic acid alone can inhibit tumor cell proliferation and clone formation with time dependence. Compared with the control, paclitaxel alone can significantly suppress tumor cell proliferation and clone formation (P < 0.05). Lipoic acid and paclitaxel in combination obviously strengthened their individual inhibitory effects on tumor cells (P < 0.05). Compared with 17935 Lipoic acid combined with paclitaxel on breast cancer ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 17934-17940 (2015) the paclitaxel alone group, the combination group exhibited more remarkable inhibitory effect (P < 0.05). Lipoic acid alone or combined with paclitaxel can inhibit NF-κB expression and inhibit breast cancer cell proliferation.

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“…25 Briefly, F68-VES/MIT micellar solutions containing 20%, 30%, 40%, and 50% FBS were prepared and incubated at 37°C for different time intervals.…”

Section: 23mentioning

confidence: 99%

Vitamin E succinate-conjugated F68 micelles for mitoxantrone delivery in enhancing anticancer activity

Liu¹,

Xu²,

Wu³

et al. 2016

IJN

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Mitoxantrone (MIT) is a chemotherapeutic agent with promising anticancer efficacy. In this study, Pluronic F68-vitamine E succinate (F68-VES) amphiphilic polymer micelles were developed for delivering MIT and enhancing its anticancer activity. MIT-loaded F68–VES (F68–VES/MIT) micelles were prepared via the solvent evaporation method with self-assembly under aqueous conditions. F68–VES/MIT micelles were found to be of optimal particle size with the narrow size distribution. Transmission electron microscopy images of F68–VES/MIT micelles showed homogeneous spherical shapes and smooth surfaces. F68–VES micelles had a low critical micelle concentration value of 3.311 mg/L, as well as high encapsulation efficiency and drug loading. Moreover, F68–VES/MIT micelles were stable in the presence of fetal bovine serum for 24 hours and maintained sustained drug release in vitro. Remarkably, the half maximal inhibitory concentration (IC 50 ) value of F68–VES/MIT micelles was lower than that of free MIT in both MDA-MB-231 and MCF-7 cells (two human breast cancer cell lines). In addition, compared with free MIT, there was an increased trend of apoptosis and cellular uptake of F68–VES/MIT micelles in MDA-MB-231 cells. Taken together, these results indicated that F68–VES polymer micelles were able to effectively deliver MIT and largely improve its potency in cancer therapy.

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Inhibition of metastasis and growth of breast cancer by pH-sensitive poly (β-amino ester) nanoparticles co-delivering two siRNA and paclitaxel

Cited by 133 publications

References 53 publications

siRNA-loaded poly(histidine-arginine)<sub>6</sub>-modified chitosan nanoparticle with enhanced cell-penetrating and endosomal escape capacities for suppressing breast tumor metastasis

siRNA-loaded poly(histidine-arginine)<sub>6</sub>-modified chitosan nanoparticle with enhanced cell-penetrating and endosomal escape capacities for suppressing breast tumor metastasis

Effect of lipoic acid combined with paclitaxel on breast cancer cells

Vitamin E succinate-conjugated F68 micelles for mitoxantrone delivery in enhancing anticancer activity

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