Effect of lipoic acid combined with paclitaxel on breast cancer cells

Abstract

ABSTRACT. Breast cancer is the most common gynecologic tumor globally that threatens women's health. Lipoic acid is a type of antioxidant that can alleviate oxidative stress damage. Studies showed that lipoic acid could inhibit the proliferation of tumor cells in cervical cancer and colon cancer. This paper intends to explore the combined effect of lipoic acid and paclitaxel on breast cancer cells. Breast cancer MCF-7 cells were divided into four groups: control group, lipoic acid group, paclitaxel group, and a combination group. MTT was applied to detect the drugs' influence on breast cancer cell proliferation. A colony formation test was used to determine the effects on breast cancer cell clone formation rate. Western blot was performed to detect the effects on nuclear factor (NF)-κB. Lipoic acid alone can inhibit tumor cell proliferation and clone formation with time dependence. Compared with the control, paclitaxel alone can significantly suppress tumor cell proliferation and clone formation (P < 0.05). Lipoic acid and paclitaxel in combination obviously strengthened their individual inhibitory effects on tumor cells (P < 0.05). Compared with 17935 Lipoic acid combined with paclitaxel on breast cancer ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 17934-17940 (2015) the paclitaxel alone group, the combination group exhibited more remarkable inhibitory effect (P < 0.05). Lipoic acid alone or combined with paclitaxel can inhibit NF-κB expression and inhibit breast cancer cell proliferation.

Alphalipoic Acid Prevents Oxidative Stress and Peripheral Neuropathy in Nab-Paclitaxel-Treated Rats through the Nrf2 Signalling Pathway

“…Therefore, we established a subcutaneous xenograft model of pancreatic cancer and found that α -LA had a potential antitumor effect, although the antitumor effect was not significant when combined with nab-PTX. Several studies also indicated that α -LA could sensitize lung cancer cells to chemotherapeutic agents [54], promote synergistic antitumor effects [55], and inhibit breast cancer cell proliferation [56].…”

Inhibitory Activity of Iron Chelators ATA and DFO on MCF-7 Breast Cancer Cells and Phosphatases PTP1B and SHP2

“…Previous studies showed that compounds with iron chelating properties may have significant impact in cancer metabolism and can be considered in chemotherapy (16,19). Recently we have shown, among other groups, that lipoic acid (LA) has inhibitory effect on MCF-7 cell viability and proliferation (24)(25)(26)(27), as well as on activity of pro-oncogenic phosphatases PTP1B and SHP2 (27). Lipoic acid is also known to has metal-chelating properties, which can be possible anticancer mechanism of LA action (28).…”

Alter between gut bacteria and blood metabolites and the anti-tumor effects of Faecalibacterium prausnitzii in breast cancer

“…Once the cancer cells fall off, the free cancer cells can spread with the blood or lymph, forming a metastasis and endangering life. Breast cancer has become a common tumor that threatens women's physical and mental health [4]. Early detection and early diagnosis of breast cancer is the key to improve the efficacy [5].…”