Alter between gut bacteria and blood metabolites and the anti-tumor effects of Faecalibacterium prausnitzii in breast cancer

Abstract

Background: The aim was to evaluate the changes of 16S rDNA sequencing and LC-MS metabolomics in breast cancer and explore the growth inhibition of breast cancer cells by Faecalibacterium prausnitzii. Results: Total 49 significantly different flora and 26 different metabolites were screened between two groups, and the correlation was calculated. Relative abudance of Firmicutes and Bacteroidetes were decreased, while relative abundance of verrucomicrobla, proteobacteria and actinobacteria was increased in breast cancer group. Differentially expressed metabolites were mainly enriched in pathways such as linoleic acid metabolism, retrograde endocannabinoid signaling, biosynthesis of unsaturated fatty acids, choline metabolism in cancer and arachidonic acid metabolism. Lipid upregulation was found in breast cancer patients, especially phosphorocholine. The abundance of Faecalibacterium was reduced in breast cancer patients, which was negatively correlated with various phosphorylcholines. Moreover, Faecalibacterium prausnitzii, the most well-known species in Faecalibacterium genus, could inhibit the secretion of interleukin-6 (IL-6) and the phosphorylation of Janus kinases 2 (JAK2)/signal transducers and activators of transcription 3 (STAT3) in breast cancer cells. Faecalibacterium prausnitzii also suppressed the proliferation and invasion and promoted the apoptosis of breast cancer cells, while these effects disappeared after adding recombinant human IL-6. Conclusions: Flora-metabolites combined with the flora-bacteria (such as Faecalibacterium combined with phosphorocholine) might a new detection method for breast cancer. Faecalibacterium may be helpful for prevention of breast cancer. Faecalibacterium prausnitzii suppresses the growth of breast cancer cells through inhibition of IL-6/STAT3 pathway.

CENPL, ISG20L2, LSM4 and MRPL3 Are Four Novel Hub Genes Involved in Breast Cancer Development and Progression

“…Among them, COL11A1, S100P, LEP and FGF2 are the most signi cant DEGs, which have been reported to be associated with the pathogenesis and development of breast cancer [38][39][40][41]. Functional annotation analysis revealed that the robust DEGs signi cantly enriched in many GO terms associated with proliferation and energy metabolism, such as extracellular matrix organization, extracellular structure organization, mitotic nuclear division, regulation of lipid metabolic process, and glycosaminoglycan binding, which were consistent with published data [42][43][44]. Moreover, the KEGG pathway enrichment analysis showed that those robust DEGs were most associated with PI3K−Akt signaling pathway, which serves as a pivotal intracellular signaling path that plays a crucial role in cell apoptosis and thus in breast cancer development [45,46].…”

Association of Bacteroides acidifaciens relative abundance with high-fibre diet-associated radiosensitisation

“…Moreover, B. acidifaciens in vitro has a greater effect on cell kill than F. prausnitzii (butyrate-producer; p < 0.001), implying that metabolites other than butyrate may be involved in its effect. Studies suggest that a broad range of gut microbiota-derived metabolites can enhance anti-tumoural effects or tumour response to anti-cancer treatments [ 35 – 37 ], so future work should include undertaking a global metabolomic analysis of B. acidifaciens -produced metabolites to identify radiosensitisers other than SCFAs with similar profiles.…”

Integrative Analysis of Vaginal Microorganisms and Serum Metabolomics in Rats With Estrous Cycle Disorder Induced by Long-Term Heat Exposure Based on 16S rDNA Gene Sequencing and LC/MS-Based Metabolomics

“…The entire genomic DNA was separated by the previously described hexadecyl trimethyl ammonium bromide (CTAB)/sodium dodecyl sulfate (SDS) method ( Ma et al, 2020 ). The consolidation and purity of DNA were monitored on 1% agarose gels.…”