Inhibition of Intercellular Communication by 2,3,7,8-Tetrachlorodibenzo-p-dioxin and Dioxin-Like PCBs in Mouse Hepatoma Cells (Hepa1c1c7): Involvement of the Ah Receptor

Integrated Biomonitoring of Dioxin-like Compounds for Waste Management and Environment.

“…In vitro micro-EROD assays have been simplified using culture cells such as H4IIE rat hepatoma cell line, mouse Hepa lclc7 cell line, the PLHC-1 fish hepatoma cell line, rainbow trout liver cell line RTL-W1 and human hepatoma HepG2 cells in 96-well microplates for the purpose more related to analysis of PHAHs [9][10][11][12][13][14][15][16][17][18][19] . For in vitro assay, induction of EROD activity caused by test samples can be compared with the induction curve of TCDD standard to determine biological TEQ (EROD-TEQ) values in samples.…”

Inhibition of Cell‐Cell Communication by Commercial Chlorinated Paraffins in Rat Liver Epithelial IAR 20 Cells

“…The induction of peroxisome proliferation has been discussed to be involved in the mechanism for hepatocarcinogenesis (Elcombe et al 1994), and the potential hazard for humans has been questioned. However, based on the data presented in this study, it appears that short and intermediate chain length chlorinated paraffins share properties with the organochlorine compounds, dioxins, PCBs and DDT, which are shown to be inhibitors of intercellular communication in vitro in rat liver epithelial cells (Flodstrom et al 1990;Hemming et al 1991;DeHaan et al 1994). The mechanism for inhibition caused by these compounds has not been clarified, although data suggest that alterations of the phosphorylation pattern of cx43 are involved (Ruch et al 1994; Y.…”

“…A number of chlorinated organic pollutants, such as dioxins, polychlorinated biphenyls (PCBs), 1,lf-(2,2,2-trichloroethylidene)bis(4-~hlorobenzene) (DDT), have been shown to be potent rat liver tumour promoters (Flodstrom et al 1990;Wzrn et al 1991;Hemming et al 1993). They also inhibit gap junctional intercellular communication in cultured rodent liver cells (Flodstrom et al 1990;Hemming et al 1991;DeHaan et al 1994;Baker et al 1995), which is a common property of many tumour promoters (Budunova 1994). Gap junctional intercellular communication is believed to be involved in the control of cell growth and differentiation (Loewenstein 1990;Warner 1992).…”

The effects of 3-methylcholanthrene on macrophage respiratory burst and biotransformation activities in the common carp (Cyprinus carpio L.)

“…Alpha-NF was used in this study to determinate whether the Ah-R was involved in 3-MC-mediated immunotoxicity [21,[52][53][54][55]. In contrast to 3-MC, alpha-NF did not stimulate the production of cytochrome P450, EROD or GST activities and macrophage respiratory burst.…”