Inhibition of in vitro neutrophil responses to chemotactic factors by piroxicam.

Tánaka, Keiichi; Kanaoka, Keiko; EGAWA, Mihoko; Abe, Norio; Watanabe, Isao; Hirai, Shiro

doi:10.1254/jjp.35.181

Cited by 8 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TANAKA et al [23] described an inhibition of [3H]-fMLP binding to PMN but our observations obtained with two different chemoattractants led us to conclude to an interaction with at least two types of specific receptors and perhaps to a non specific modification of PMN membrane.…”

Section: Discussioncontrasting

confidence: 56%

“…An inhibition by piroxicam of rabbit PMN chemotaxis and O; generation induced by various stimuli (fMLP, concanavalin A, ionophore A 23187, activated serum) has already been described by TANAKA et al [23]. These authors suggest that the effect observed with 0 2 generation cannot be due to the interference of receptor-ligand binding but is likely to be due to the inhibition of an activation step of the 0 2 generating system of PMN common to all the stimuli used.…”

Section: Discussionmentioning

confidence: 72%

See 1 more Smart Citation

In vitro andin vivo effects of piroxicam on rat polymorphonuclear responsiveness after induction of two acute non-specific inflammatory reactions

et al. 1986

View full text Add to dashboard Cite

The effect of piroxicam on rat polymorphonuclear leucocytes (PMN) has been studied in vitro and in vivo after the induction of two acute, non specific inflammatory reactions (pleurisies induced by calcium pyrophosphate crystals (CaPP) or isologous serum). An inhibition of chemotaxis by piroxicam has been demonstrated by two techniques, the filter and agarose assays in vivo and in vitro. An inhibition of random cell migration has been observed only at the higher drug concentration using agarose assay with CaPP-elicited cells. Piroxicam also inhibited superoxide anion generation and O2 consumption of CaPP- and serum-elicited cells. These findings suggest that piroxicam may have a direct effect on PMN responses and that this activity could, at least in part, contribute to its anti-inflammatory properties.

show abstract

Section: Discussioncontrasting

confidence: 56%

Section: Discussionmentioning

confidence: 72%

In vitro andin vivo effects of piroxicam on rat polymorphonuclear responsiveness after induction of two acute non-specific inflammatory reactions

et al. 1986

View full text Add to dashboard Cite

show abstract

“…However, Tanaka et al (1984), using Boyden's chamber technique, showed that with 10 pmol L-I indomethacin (about 4 p g mL-l), chemotaxis in response to FMLP is inhibited by 19.6%. We found a 10.2% inhibition at this concentration, using the technique of migration under agarose gel.…”

Section: Discussionmentioning

confidence: 98%

Effects of indomethacin and diclofenac on some functions of polymorphonuclear neutrophils

Berradia¹,

Marchand-Arvier²,

Humbert³

et al. 1988

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

The effects in-vitro of two non-steroidal anti-inflammatory drugs, diclofenac and indomethacin, have been studied on human polymorphonuclear functions. Adhesivity and chemotaxis were decreased by the two drugs, but in a manner that varied with the attractant used. As shown by the nitro blue tetrazolium slide test, all cells remained active but the production of oxygen metabolites by opsonized zymosan was decreased in the presence of diclofenac.

show abstract

“…We demonstrated in vivo that indomethacin inhibited the IL-8-induced infiltration of leukocytes. Indomethacin was also reported to inhibit adhesivity and chemotaxis of neutrophils in response to zymosan-activated serum or N-formyl-methionyl-leucyl-phenylalanine (FMLP) [26] and the release of lysosomal enzymes and the production of superoxide anions by neutrophils [27].…”

Section: Discussionmentioning

confidence: 99%

Involvement of interleukin-8 in dialysis-related arthritis

Takayama

Miyazaki

Aoyama

et al. 1998

Kidney International

View full text Add to dashboard Cite

To elucidate the role of interleukin (IL)-8, a chemotactic factor for neutrophils, in dialysis-related arthritis (DRA) of patients on long-term hemodialysis, the concentration of IL-8 was measured in the synovial fluids of DRA patients with acute arthralgia and joint swelling, and was compared with those in patients with rheumatoid arthritis (RA) and patients with osteoarthritis (OA). We noted a marked elevation of IL-8 in the joint fluids of patients with DRA and RA as compared with OA. Furthermore, to determine the role of IL-8 in synovitis, we examined the in vivo effect of intra-articular injection of human recombinant IL-8 on leukocyte infiltration into the joint space of rabbits. A single injection of IL-8 to the joints of rabbits induced rapid infiltration of neutrophils into the joint space and synovial tissues, which reached a maximum in four hours. The oral administration of indometacin farnesil (a prodrug that is converted to indomethacin after intestinal absorption) before the injection of IL-8 alleviated the infiltration of neutrophils. When human synovial cells were incubated with tumor necrosis factor (TNF)-alpha, the expression of IL-8 mRNA and IL-8 production in the cultured synovial cells were increased. The TNF-alpha-stimulated expression of IL-8 mRNA and IL-8 production in the cultured synovial cells were markedly inhibited by dexamethasone. In conclusion, IL-8 levels were markedly elevated in the joint fluids of patients with DRA. Interleukin-8 released from synovial cells may be an important factor to induce acute inflammation in DRA. Dexamethasone and indomethacin may be effective for DRA by inhibiting the production and chemotactic actions of IL-8, respectively.

show abstract

Inhibition of in vitro neutrophil responses to chemotactic factors by piroxicam.

Cited by 8 publications

References 34 publications

In vitro andin vivo effects of piroxicam on rat polymorphonuclear responsiveness after induction of two acute non-specific inflammatory reactions

In vitro andin vivo effects of piroxicam on rat polymorphonuclear responsiveness after induction of two acute non-specific inflammatory reactions

Effects of indomethacin and diclofenac on some functions of polymorphonuclear neutrophils

Involvement of interleukin-8 in dialysis-related arthritis

Contact Info

Product

Resources

About