Inhibition of IL-6 and IL-8 production in LPS-stimulated human gingival fibroblasts by glycyrrhizin via activating LXRα

Zhang, Na; Lv, Hui; Shi, Bo-Hong; Hou, Xin; Xu, Xin

doi:10.1016/j.micpath.2017.06.021

Cited by 11 publications

(9 citation statements)

References 25 publications

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“…In a few cases, this final WB analysis has been moved to following figures because additional graphs have been added in preceding positions. It is the case with Zhang et al, where the WB for LXRa is in Figure 5 because a WB for iNOS and COX-2 occupies Figure 3 [16], Liu et al, where the WB for p-PI3K and p-AKT is in Figure 5 because PGE 2 and nitrites levels were reported in Figure 2 [18] or Hao et al [17], where we find a WB for PPAR-g is shown in Figure 7.3.1.…”

Section: Figuresupporting

confidence: 65%

“…In one instance [15], Figure 2 includes the mRNA levels for IL-8 and IL-6 beside the data for these same ILs from the ELISA assay, side by side. Interestingly, this figure is most commonly composed of two graphs vertically ordered, with IL-8 on top and IL-6 at the bottom [10,12,16,19]. In one instance [15], Figure 2 includes the mRNA levels for IL-8 and IL-6 beside the data for these same ILs from the ELISA assay, side by side.…”

Section: Figurementioning

confidence: 99%

“…Figure 2 of the template focuses on the effectiveness of the X compound to reduce the production of pro-inflammatory mediators, mostly Interleukins (ILs) [34]. Prostaglandins have long been recognized as important molecular players in periodontal inflammation [35,36], and it is, therefore, not surprising that PGE 2 or cyclooxygenase-2, the enzyme that synthesizes it, are included in the array of relevant endpoints in several papers that follow this format [8,[16][17][18]. As LPS has been shown to increase the expression and release of a vast array of ILs; it is important to pick those that are likely to have more profound repercussions on the development of periodontal diseases.…”

Section: Establishing a Traditionmentioning

confidence: 99%

“…P. Gingivalis is one of the most extensively studied microorganisms in the pathogenesis of periodontitis and has been shown to elicit both a potent 3.1. Figure 1 The first figure of the template (Figure 1) is usually a viability assay, e.g., MTT [8,10,12,[15][16][17][18] or, in a few cases, CCK-8 [19,20] assay that compares the effects of the X compound, alone or in combination with LPS, on HGF viability at different concentrations, plotted as a bar chart. This figure is possibly the most consistent of the whole format and it is found in virtually all the studies that follow this template.…”

Section: Introductionmentioning

confidence: 99%

“…In one case, this legend is reproduced so precisely that it includes the term MTT, when the assay used in the study was actually CCK-8, according to the Materials and Methods Section [20] Interestingly, standard deviation was chosen as an indicator of dispersion in only two cases [15,16].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Between Innovation and Standardization, Is There Still a Room for Scientific Reports? The Rise of a Formatting Tradition in Periodontal Research

et al. 2019

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Everybody, regardless of their role, is aware that biomedical research is rapidly evolving, and the demand for reproducibility is increasing together with the amount of novel information. “Before reproducibility must come pre-producibility” “Checklists work to improve science”, just to quote some of the articles querying how to find a new bridge between ethics in science and the urgency for publishing. Looking for papers on anti-inflammatory compounds in periodontics, we came across a significant number of articles that could be considered a prototype of a consistent study format. The literature on the testing of active compounds on lipopolysaccharides- (LPS)-induced inflammation in gingival fibroblasts was searched to identify studies that followed a consistent format, to better understand their similarities and assess the appropriateness of their methods. Several studies were identified with a degree of similarity in their methods and formatting that was so high that it was possible to rule out that it was due to chance, and a format template common to these studies was outlined. Although this was most likely beyond the intentions of their authors, these studies may pose the basis for an in-vitro testing standard for anti-inflammatory compounds; however, the dangers of acritical uniformity are also apparent.

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Section: Figuresupporting

confidence: 65%

Section: Figurementioning

confidence: 99%

Section: Establishing a Traditionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Between Innovation and Standardization, Is There Still a Room for Scientific Reports? The Rise of a Formatting Tradition in Periodontal Research

et al. 2019

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Peroxisome proliferator activated receptor γ promotes mineralization and differentiation in cementoblasts via inhibiting Wnt/β‐catenin signaling pathway

Wang

et al. 2019

J of Cellular Biochemistry

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Peroxisome proliferator activated receptor γ (PPARγ) is a member of the nuclear receptor family of transcription factors, which involved in inflammation regulating and bone remodeling. Rare studies explored the effects of PPARγ on mineralization and differentiation in cementoblasts. To explore the potential approaches to repair the damaged periodontal tissues especially for cementum, the present study aims to investigate the effects and the regulating mechanism of PPARγ on mineralization and differentiation in cementoblasts. Murine cementoblast cell lines (OCCM‐30) were cultured in basic medium for 24 hours/48 hours or in mineralization medium for 3/7/10 days, respectively at addition of dimethyl sulphoxide, rosiglitazone (PPARγ agonist), GW9662 (PPARγ antagonist), lithium chloride (LiCl), tumor necrosis factor‐α (TNF‐α), or respective combination. Expression of mineralization genes alkaline phosphatase (ALP), runt related transcription factors 2 (RUNX2), and osteocalcin (OCN) were detected by quantitative real‐time polymerase chain reaction or/and Western blot. ALP staining and alizarin red staining were used to evaluate the mineralization in OCCM‐30 cells. The change of β‐catenin expression and translocation in cytoplasm/nucleus was analyzed by Western blot and immunofluorescence. The results showed that PPARγ agonist rosiglitazone improved the expression of ALP, RUNX2, and OCN, deepened ALP staining, increased mineralized nodules formation, and decreased β‐catenin expression in the nucleus. LiCl, an activator of the Wnt signaling pathway, inhibited the expression of mineralization genes and reversed the upregulated expression of mineralization genes resulted from rosiglitazone. Under inflammatory microenvironment, rosiglitazone not only suppressed the expression of interleukin‐1β caused by TNF‐α, but improved the expression of mineralization genes in OCCM‐30 cells. In conclusion, PPARγ could promote mineralization and differentiation in cementoblasts via inhibiting the Wnt/β‐catenin signaling pathway, which would shed new light on the treatment of periodontitis and periodontal tissue regeneration.

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Anti-inflammatory effect of glycyrrhizin with Equisetum arvense extract

Shiba¹,

Miyauchi

Chea

et al. 2020

Odontology

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Inhibition of IL-6 and IL-8 production in LPS-stimulated human gingival fibroblasts by glycyrrhizin via activating LXRα

Cited by 11 publications

References 25 publications

Between Innovation and Standardization, Is There Still a Room for Scientific Reports? The Rise of a Formatting Tradition in Periodontal Research

Between Innovation and Standardization, Is There Still a Room for Scientific Reports? The Rise of a Formatting Tradition in Periodontal Research

Peroxisome proliferator activated receptor γ promotes mineralization and differentiation in cementoblasts via inhibiting Wnt/β‐catenin signaling pathway

Anti-inflammatory effect of glycyrrhizin with Equisetum arvense extract

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