Anti-inflammatory effect of glycyrrhizin with Equisetum arvense extract

Shiba, Fumie; Miyauchi, Mutsumi; Chea, Chanbora; Furusho, Hisako; Iwasaki, Saori; Shimizu, Rika; Ohta, Kouji; Nishihara, Tatsuji; Takata, Takashi

doi:10.1007/s10266-020-00563-3

Cited by 9 publications

(4 citation statements)

References 31 publications

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“…Previously, by using a rat model of LPS-induced periodontitis, we confirmed that administration of EA into the gingival sulcus markedly reduced immunoexpression of TNF- α at the junctional epithelium [ 29 ]. TNF- α promotes the increase of osteoclast progenitor cells in vivo [ 30 – 33 ], and inhibition of human osteoclastogenesis in vitro by EA has also been reported [ 10 , 34 ]; therefore, we suggested that EA could inhibit alveolar bone destruction associated with periodontitis.…”

Section: Introductionmentioning

confidence: 56%

“…Furthermore, TNF-α and IL-1β have been reported to act on osteoblasts to increase Fas expression and induce apoptosis [49]. Previously, by using the rat model of LPSinduced periodontitis, we confrmed that EA treatment markedly reduced immunoexpression of TNF-α at the junctional epithelium [29]. In the present study, evaluation using osteoblast lineage cells demonstrated the strong inhibitory efect of EA on E. coli-LPS-induced production of infammatory cytokines (TNF-α, IL-1β, and IL-6).…”

Section: Discussionmentioning

confidence: 91%

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Equisetum arvense Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis

Shiba

Furusho

Takata

et al. 2022

International Journal of Dentistry

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Background and Aims. Equisetum arvense extract (EA) exerts various biological effects, including anti-inflammatory activity. The effect of EA on alveolar bone destruction has not been reported; therefore, we aimed to determine whether EA could inhibit alveolar bone destruction associated with periodontitis in a rat model in which periodontitis was induced using lipopolysaccharide from Escherichia coli (E. coli-LPS). Methods. Physiological saline or E. coli-LPS or E. coli-LPS/EA mixture was topically administered into the gingival sulcus of the upper molar region of the rats. After 3 days, periodontal tissues of the molar region were collected. Immunohistochemistry was performed for cathepsin K, receptor activator of NF-κB ligand (RANKL), and osteoprotegerin (OPG). The cathepsin K-positive osteoclasts along the alveolar bone margin were counted. EA effects on the expression of the factors regulating osteoclastogenesis in osteoblasts with E. coli-LPS-stimulation were also examined in vitro. Results. Treatment with EA significantly reduced the number of osteoclasts by decreasing the RANKL-expression and increasing OPG-expression in the periodontal ligament in the treatment group compared to the E. coli-LPS group. The in vitro study showed that the upregulation of p-IκB kinase α and β (p-IKKα/β), p-NF-κB p65, TNF-α, interleukin-6, and RANKL and downregulation of semaphorin 3A (Sema3A), β-catenin, and OPG in the osteoblasts with E. coli-LPS-stimulation improved with EA-treatment. Conclusion. These findings demonstrated that topical EA suppressed alveolar bone resorption in the rat model with E. coli-LPS-induced periodontitis by maintaining a balance in RANKL/OPG ratio via the pathways of NF-κB, Wnt/β-catenin, and Sema3A/Neuropilin-1. Therefore, EA possesses the potential to prevent bone destruction through inhibiting osteoclastogenesis attributed to cytokine burst under plaque accumulation.

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Section: Introductionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 91%

Equisetum arvense Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis

Shiba

Furusho

Takata

et al. 2022

International Journal of Dentistry

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“… 2019 ; Bailly and Vergoten 2020 ; Shiba et al. 2021 ). The mechanism of GL protecting cells from influenza A virus (IAV) infection was studied, and the antiviral effect of GL on a variety of IAV infected cells was demonstrated.…”

Section: Antiviral Mechanisms Of Traditional Chinese Medicinesmentioning

confidence: 99%

Research progress on antiviral constituents in traditional Chinese medicines and their mechanisms of action

Chen

2022

Pharmaceutical Biology

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Context Viruses have the characteristics of rapid transmission and high mortality. At present, western medicines still lack an ideal antiviral. As natural products, many traditional Chinese medicines (TCM) have certain inhibitory effects on viruses, which has become the hotspot of medical research in recent years. Objective The antiviral active ingredients and mechanisms of TCM against viral diseases was studied in combination with the pathogenesis of viral diseases and antiviral effects. Materials and methods English and Chinese literature from 1999 to 2021 was collected from databases including Web of Science, PubMed, Elsevier, Chinese Pharmacopoeia 2020 (CP), and CNKI (Chinese). Traditional Chinese medicines (TCM), active ingredients, antiviral, mechanism of action, and anti-inflammatory effect were used as the key words. Results The antiviral activity of TCM is clarified to put forward a strategy for discovering active compounds against viruses, and provide reference for screening antivirus drugs from TCM. TCM can not only directly kill viruses and inhibit the proliferation of viruses in cells, but also prevent viruses from infecting cells and causing cytophilia. It can also regulate the human immune system, enhance human immunity, and play an indirect antiviral role. Discussion and conclusion Based on the experimental study and antiviral mechanism of TCM, this paper can provide analytical evidence that supports the effectiveness of TCM in treating virus infections, as well as their mechanisms against viruses. It could be helpful to provide reference for the research and development of innovative TCMs with multiple components, multiple targets and low toxicity.

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“…This extract also leads to a reduction in JNK phosphorylation, a factor involved in immune responses. Quercetin 3-O-glucoside in E. arvense has been suggested to inhibit JNK phosphorylation, resulting in an anti-inflammatory effect [20]. Furthermore, when the extract of E. arvense was topically applied in a rat periodontitis model, it effectively inhibited bone loss by suppressing osteoclast formation triggered by several inflammatory cytokines, including TNFα, IL-1β, and IL-6.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Investigation of Equisetum arvense and Evaluation of Their Anti-Inflammatory Potential in TNFα/INFγ-Stimulated Keratinocytes

Jeong,

Yu,

et al. 2023

Pharmaceuticals

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Equisetum arvense L. (Equisetaceae), widely known as ‘horsetail’, is a perennial plant found extensively across Asia. Extracts of E. arvense have been used in traditional medicine, particularly for the treatment of inflammatory disorders. This study aimed to determine the phytochemical compounds in E. arvense ethanolic extract and their anti-inflammatory properties. Subsequently, we isolated and identified nine secondary metabolites, including kaempferol 3,7-di-O-β-D-glucopyranoside (1), icariside B2 (2), (Z)-3-hexenyl β-D-glucopyranoside (3), luteolin 5-O-β-D-glucopyranoside (4), 4-O-β-D-glucopyranosyl caffeic acid (5), clemastanin B (6), 4-O-caffeoylshikimic acid (7), (7S,8S)-threo-7,9,9′-trihydroxy-3,3′-dimethoxy-8-O-4′-neolignan-4-O-β-D-glucopyranoside (8), and 3-O-caffeoylshikimic acid (9). The chemical structures of the isolated compounds (1–9) were elucidated using HR-ESI-MS data, NMR spectra, and ECD data. Next, the anti-inflammatory effects of the isolates were evaluated in tumor necrosis factor (TNF)α/interferon (IFN)γ-induced HaCaT, a human keratinocyte cell line. Among the isolates, compound 3 showed the highest inhibitory effect on the expression of pro-inflammatory chemokines, followed by compounds 6 and 8. Correspondingly, the preceding isolates inhibited TNFα/IFNγ-induced activation of pro-inflammatory transcription factors, signal transducer and activator of transcription 1, and nuclear factor-κB. Collectively, E. arvense could be employed for the development of prophylactic or therapeutic agents for improving dermatitis.

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Anti-inflammatory effect of glycyrrhizin with Equisetum arvense extract

Cited by 9 publications

References 31 publications

Equisetum arvense Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis

Equisetum arvense Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis

Research progress on antiviral constituents in traditional Chinese medicines and their mechanisms of action

Phytochemical Investigation of Equisetum arvense and Evaluation of Their Anti-Inflammatory Potential in TNFα/INFγ-Stimulated Keratinocytes

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