Inhibition of Listeria monocytogenes in Cooked Ham by Virulent Bacteriophages and Protective Cultures

Abstract

Protective cultures can be used successfully as an additional hurdle together with phages to reduce growth of Listeria monocytogenes on sliced cooked ham. Addition of phages resulted in a rapid 10-fold reduction of L. monocytogenes. After 14 to 28 days of storage, a 100-fold reduction was observed in samples with phages and protective culture compared to results for samples with phages alone.

Use of epifluorescence microscopy to assess the effectiveness of phage P100 in controlling Listeria monocytogenes biofilms on stainless steel surfaces

“…However, a possible disadvantage is that increasing the rounds of replication could result in the development of host resistance (El-Shibiny et al, 2009). However, some other previous studies found no evidence of phage P100 resistance in bacteria treated survivors (Carlton et al, 2005;Holck & Berg, 2009).…”

Resistance of foodborne pathogen coliphages to thermal and physicochemical treatments applied in food manufacture

“…Bacteriophages are the natural enemies of bacteria and have proved to be useful tools against pathogenic E. coli strains (Abuladze et al, 2008;Moradpour et al, 2009;O'Flynn, Ross, Fitzgerald, & Coffey, 2004) and several other foodborne pathogens, such as Listeria monocytogenes (Carlton, Noordman, Biswas, de Meester, & Loessner, 2005;Dykes & Moorhead, 2002;Guenther, Huwyler, Richard, & Loessner, 2009;Holck & Berg, 2009), Campylobacter jejuni (Bigwood, Hudson, Billington, Carey-Smith, & Heinemann, 2008), Salmonella enterica (Bigwood et al, 2008) and Staphylococcus aureus (Bueno, Garcia, Martínez, & Rodríguez, 2012;Garcia, Madera, Martinez, & Rodriguez, 2007;Obeso et al, 2010). However, viability and activity of these phages must be assessed under typical physicochemical conditions found in each food matrix in order to evaluate real effectiveness of phages on these complex environments.…”

Silage Collected from Dairy Farms Harbors an Abundance of Listeriaphages with Considerable Host Range and Genome Size Diversity

“…Similarly, Kim et al (35) reported that 9/12 listeriaphages isolated from two turkey processing plants were characterized as broad-host-range phages, exhibiting the ability to lyse the majority of L. monocytogenes serotype 1/2a strains (16/26) and 4b strains (38/39). A number of listeriaphages from these and other studies have been well characterized, including by genome sequencing (10,36,64), and have been developed for biocontrol and other applications, such as phage A511 (27, 28) and P100 (10,28,51).Recent studies suggest potential uses of listeriaphage as a biocontrol agent for L. monocytogenes in a variety of ready-to-eat (RTE) foods (10,28,31,37,38,51). Some studies have also suggested the suitability of phage applications in controlling foodborne pathogens at the preharvest level and reducing shedding in animals (8, 9, 52).…”

“…Recent studies suggest potential uses of listeriaphage as a biocontrol agent for L. monocytogenes in a variety of ready-to-eat (RTE) foods (10,28,31,37,38,51). Some studies have also suggested the suitability of phage applications in controlling foodborne pathogens at the preharvest level and reducing shedding in animals (8,9,52).…”