Inhibition ofHelicobacter pyloriand Its Associate Urease by Labdane Diterpenoids Isolated fromAndrographis paniculata

Abstract

The present study was carried out to evaluate anti-Helicobacter pylori and its associated urease activity of labdane diterpenoids isolated from Andrographis paniculata. A molecular docking analysis was performed by using ArgusLab 4.0.1 software. The results obtained indicate that compound A possesses strong inhibition to H. pylori, 28 ± 2.98 (minimum inhibitory concentration, 9 µg/mL), and its urease, 85.54 ± 2.62% (IC50 , 20.2 µg/mL). Compounds B, C, and D also showed moderate inhibition to H. pylori and its urease. The obtained results were in agreement with the molecular docking analysis of compounds. The phytochemicals under investigation were found to be promising antibacterial agents. Moreover, the isolated compounds can be considered as a resource for searching novel anti-H. pylori agents possessing urease inhibition.

Antioxidant, anti‐inflammatory, and enzyme inhibitory activity of natural plant flavonoids and their synthesized derivatives

“…However, luteolin, apigenin, diosmetin, O 4Ꞌ , O 7 ‐dihexyl apigenin, and chrysin exhibited significant inhibition toward urease compared with all other flavonoids. Several bioactive phenolic compounds were studied which were exhibiting a significant inhibitory effect and which have been identified from plant materials, among them are flavonoids, tannins, terpenes, aromatic aldehydes, alcohols, tannins, and catechins that are studied widely . Thus, the results are in accordance with reported studies.…”

“…Several bioactive phenolic compounds were studied which were exhibiting a significant inhibitory effect and which have been identified from plant materials, among them are flavonoids, tannins, terpenes, aromatic aldehydes, alcohols, tannins, and catechins that are studied widely. [35] Thus, the results are in accordance with reported studies. The literature reported that flavonoids showed significant urease activity, and our results are in close agreement with the literature data.…”

Natural Products for the Prevention and Management of Helicobacter pylori Infection

“…In vitro studies found that several components from medicinal plants inhibited the activity of H. pylori urease, such as both methanol and acetone extracts from Acacia nilotica and Calotropis procera (Amin, Anwar, Naz, Mehmood, & Saari, 2013), betulinic acid and oleanolic acid from Forsythia suspense (Shin, Park, Baek, Chung, & Park, 2009), water extract from Terminalia chebula (inhibitory concentrations: 1 to 2.5 mg/mL; Malekzadeh, Ehsanifar, Shahamat, Levin, & Colwell, 2001). Labdane diterpenoids isolated from Andrographis paniculata inhibited urease activity in vitro with 50% inhibitory concentration (IC 50 ) of 20.2 μg/mL (Shaikh et al, 2016), and for dichloromethane extract from Parthenium hysterophorus roots, the IC 50 is 136.4 μg/mL (Espinosa-Rivero, Rendon-Huerta, & Romero, 2015). Pterocarpus santalinus could inhibit the activity of urease, and decrease the lipid peroxidation and lactate dehydrogenase when co-cultivated with gastric epithelial cells of rats (Narayan, Veeraraghavan, & Devi, 2007).…”

Advances in ameliorating inflammatory diseases and cancers by andrographolide: Pharmacokinetics, pharmacodynamics, and perspective

“…214 Andrographolide sodium bisulphite showed inhibitory potency on helicobacter pylori and helicobacter pylori urease. 215,216 Andrographolide was found to suppress the expression of E-selectin at the mRNA level, reducing the adherence of gastric cancer cells to human vascular endothelial cells. 200 Treatment with andrographolide decreased proliferation and increased apoptosis in three gastric cell lines: BGC-822, SGC7901, and C666-1 depending on time and concentration.…”